Biochemistry : Catabolic Pathways and Metabolism

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #3 : Other Electron Transport Chain Concepts

Oxygen is known as the "terminal electron receptor" in the electron transport chain. Suppose an organism lacks the ability to breathe in oxygen.

What is the most likely effect an oxygen deficit would have on the electron transport chain in mitochondria?

Possible Answers:

The oxygen would not be able to interact with ATP synthase and thus ATP production would halt, however, the electron transport chain would continue to function normally otherwise.

Without a terminal electron acceptor, the electrons of  and  would have nowhere to be released, and all of the enzyme complexes involved with the electron transport chain would be "saturated" with electrons and the entire electron transport chain would cease to function, halting production of ATP.

The mitochondria would synthesize new complexes for the electron transport chain and ATP production would continue.

A mitochondria would use an alternative terminal electron receptor such as nitrogen or carbon dioxide, the electron transport chain would continue to function.

Nothing would happen, the electron transport chain would function normally.

Correct answer:

Without a terminal electron acceptor, the electrons of  and  would have nowhere to be released, and all of the enzyme complexes involved with the electron transport chain would be "saturated" with electrons and the entire electron transport chain would cease to function, halting production of ATP.

Explanation:

Without a terminal electron acceptor, the electrons of  and  would have nowhere to be released, all of the complexes would be "backed up" as each complex would not be able to pass off its electrons to the next complex. ATP production would come to a standstill.

Without oxygen to receive the electrons, the entire flow of the electron transportation chain halts, as well as ATP production. It is the continuous flow of electrons through the ETC complexes that allows a mitochondria to harness the energy of the electrons that  and  donate. This energy is used to pump protons across the intermembrane space of a mitochondria. The re-entry of these protons through ATP synthase is what drives the production of ATP.

In short, no electron flow means no proton pumps and no re-entry of those protons through ATP synthase. A cell could potentially resort to glycolysis to produce ATP, and can regenerate  or  using anaerobic fermentation such as alcohol fermentation of lactic acid fermentation.

Example Question #1 : Other Electron Transport Chain Concepts

To which component of the electron transport chain does cyanide bind?

Possible Answers:

Cyanide binds the electron transport chain at the level of coenzyme Q

Cyanide binds the electron transport chain at the level of complex IV

Cyanide binds ATP synthase

Cyanide binds the electron transport chain at the level of complex I

Cyanide binds the electron transport chain at the level of complex III

Correct answer:

Cyanide binds the electron transport chain at the level of complex IV

Explanation:

The electron transport chain passes electrons thru its main components: complex I (NADH dehydrogenase), coenzyme Q, complex III, cytochrome C, and complex IV. Complex IV is the cytochrome oxidase complex and it is inhibited by cyanide, carbon monoxide and azide. Cyanide binds irreversibly to complex IV preventing electron transfer.

Example Question #41 : Electron Transport And Oxidative Phosphorylation

Reactive oxygen species are by-products of the electron transport chain. Which of the following are considered reactive oxygen species?

Possible Answers:

All of these

None of these

Hydroxyl radical 

Hydrogen peroxide 

Superoxide 

Correct answer:

All of these

Explanation:

Reactive oxygen species are superoxide, hydrogen peroxide, and hydrogen radicals. They are degraded by catalase, superoxide dismutase, and glutathione peroxidase. Neutrophils use reactive oxygen species to kill bacteria during the phagocytic oxidative burst. 

Example Question #42 : Electron Transport And Oxidative Phosphorylation

Which of the following are uncouplers of the electron transport chain?

I. Carbon monoxide

II. 2,4-Dinitrophenol

III. Nitric oxide 

IV. Aspirin

Possible Answers:

I, III, and IV

I and II

I and III

I and IV

II and IV

Correct answer:

II and IV

Explanation:

Uncouplers of the electron transport chain decrease the proton gradient and thus decrease ATP synthesis. Most energy from the electron transport chain is released as heat. The most common uncouplers are 2,4-dinitrophenol and aspirin, as well as thermogenin. Carbon monoxide is an inhibitor of the electron transport chain, not an uncoupler. Nitric oxide does not affect directly the electron transport chain.

Example Question #181 : Catabolic Pathways And Metabolism

What is an electron acceptor in oxidative phosporylation?

Possible Answers:

ATP

Oxygen

Carbon dioxide

Carbon monoxide

NADH

Correct answer:

Oxygen

Explanation:

Oxygen is an electron acceptor. In the absence of oxygen (hypoxia) cells cannot generate ATP in the mitochondria. Instead, they will utilize glycolysis. Oxygen is required to carry out the electron transport chain and produce ATP via oxidative phosphorylation.

Example Question #911 : Biochemistry

Which of the following metabolic processes directly requires oxygen?

Possible Answers:

Pyruvate dehydrogenase complex

Glycolysis

Citric acid cycle

Tricarboxylic acid cycle

Electron transport system

Correct answer:

Electron transport system

Explanation:

The electron transport system is the only metabolic process listed that directly requires molecular oxygen. Oxygen is the final electron acceptor (it is one of the most electronegative atoms in our bodies) in the electron transport chain. This is the same as saying that oxygen has the highest reduction potential, and is capable of receiving electons. If oxygen is not present to accept the electron from the final enzyme complex in the inner mitochondrial membrane, then electron transport will be inhibited and thus no ATP will be produced via chemiosmosis.

Note that the Krebs cycle, citric acid cycle, and tricarboxylic acid cycle (TCA cycle) all refer to the same process, and do not directly require oxygen (oxygen is neither a reactant nor a product in any of the steps). However, oxygen is indirectly required, as there is no point to this cycle without subsequent oxidative phosphorylation. Thus in the absence of oxygen, of the choices shown, only glycolysis will proceed uninhibited.

Example Question #45 : Electron Transport And Oxidative Phosphorylation

Which phase of cellular respiration accounts for the highest production of energy?

Possible Answers:

Krebs Cycle

Glycolysis

Electron transport chain

Fermentation

Correct answer:

Electron transport chain

Explanation:

The electron transport chain generates the most ATP out of all three major phases of cellular respiration. Glycolysis produces a net of 2 ATP per molecule of glucose. In the Krebs cycle, there is one GTP (which is an ATP equivalent) generate in the conversion of succinyl-CoA to succinate. However, the majority of the ATP produced during cellular respiration occurs at the electron transport chain by the reduction of coenzymes NADH and . This subsequently results in the generation of the proton motive force which ATP synthase uses to generate ATP from one unit of ADP and one unit of inorganic phosphate.

Example Question #1 : Other Oxidatative Phosphorylation Concepts

Where does oxidative phosphorylation take place in a prokaryote?

Possible Answers:

Endoplasmic reticulum

Cell membrane

Nucleus

Mitochondria

Cell wall

Correct answer:

Cell membrane

Explanation:

In a eukaryote, oxidative phosphorylation occurs in the mitochondria because this is where the cell is able to set up a proton gradient.  However, prokaryotes do not have mitochondria - they have no membrane-bound organelles at all.  Therefore, the proton gradient that drives ATP synthesis in oxidative phosphorylation is created across the cell membrane.

Example Question #45 : Electron Transport And Oxidative Phosphorylation

If an uncoupler allows for excess buildup of protons inside of the mitochondrial matrix, which of the following processes will be inhibited?

Possible Answers:

Electron transport chain

Glycolysis

Krebs cycle

None of these will be inhibited

Pyruvate dehydrogenase complex

Correct answer:

None of these will be inhibited

Explanation:

With the excess buildup of protons in the matrix, the only thing that will be inhibited is the generation of ATP by ATP synthase. The other processes in cellular respiration focus more on creation of high energy electron carriers, and therefore will continue as normal.

Example Question #46 : Electron Transport And Oxidative Phosphorylation

In oxidative phosphorylation, electrons are transferred from NADH and FADH2 to electron acceptors.  This is one example of an oxidative process.  Which of the following processes within another biochemical process could be considered oxidation?

Possible Answers:

Removing hydrogens

Removing oxygen

Adding electrons

Adding hydrogens

None of these choices are oxidation

Correct answer:

Removing hydrogens

Explanation:

Predictably, a gain in oxygen is known as oxidation, while a loss of oxygen is reduction.  Hydrogen follows the opposite pattern as oxidation: removing hydrogen is oxidation, while gaining hydrogen is reduction.  Therefore, the correct answer is that removing hydrogens is considered oxidation.

In order to differentiate between oxidation and reduction in terms of electron transfer, it is helpful to remember the phrase "LEO the tiger says GER".  A loss of electrons is oxidation, while a gain of electrons is reduction. 

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