MCAT Biology : MCAT Biological Sciences

Study concepts, example questions & explanations for MCAT Biology

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Example Questions

Example Question #1221 : Mcat Biological Sciences

Type 1 diabetes is a well-understood autoimmune disease. Autoimmune diseases result from an immune system-mediated attack on one’s own body tissues. In normal development, an organ called the thymus introduces immune cells to the body’s normal proteins. This process is called negative selection, as those immune cells that recognize normal proteins are deleted. If cells evade this process, those that recognize normal proteins enter into circulation, where they can attack body tissues. The thymus is also important for activating T-cells that recognize foreign proteins.

As the figure below shows, immune cells typically originate in the bone marrow.  Some immune cells, called T-cells, then go to the thymus for negative selection. Those that survive negative selection, enter into general circulation to fight infection. Other cells, called B-cells, directly enter general circulation from the bone marrow. It is a breakdown in this carefully orchestrated process that leads to autoimmune disease, such as type 1 diabetes.

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When activated, T-cells use a number of proteins to kill cells that they recognize as foreign. A scientist develops an experimental drug to treat autoimmune disease by disrupting one of these proteins. The drug degrades the cytosolic mRNA for this protein in a T-cell. Which of the following is true if this drug is used successfully?

I. The protein is synthesized, but in an inactive form

II. The protein gene is transcribed

III. The total complement tRNA used for synthesis of the protein is not mobilized to active ribosomes

Possible Answers:

II, only

I and III

II and III

I, II, and III

III, only

Correct answer:

II and III

Explanation:

The successful use of the drug implies that the mRNA is degraded before it can be used in translation. As a result, the tRNAs used for the translation would not be mobilized for use on translating ribosomes, but transcription of mRNA would be unimpeded. The protein gene would be transcribed, the mRNA would be modified and leaves the nucleus, and would then be degraded before any synthesis could occur.

Example Question #152 : Cell Biology, Molecular Biology, And Genetics

Temperature sensitive (Ts) mutations are a powerful genetic tool in yeast and fruit flies. Ts mutations allow researchers to examine biological functions of specific genes at permissive (phenotypically normal) and restrictive (phenotypically abnormal) temperatures. What is the likely result of the Ts mutation at the restrictive temperature?

Possible Answers:

Biological processes, such as transcription and translation, are globally impaired

The protein arising from the mutated gene does not fold properly

The gene that is mutated is only required at the restrictive temperature

Cells undergo apoptosis

Correct answer:

The protein arising from the mutated gene does not fold properly

Explanation:

The temperature sensitive (Ts) mutation to a given gene results in a less stable protein product. At higher temperatures the protein does not fold properly or "melts," resulting in an improper structure of the protein. In turn, this improper structure will inhibit its function.

The Ts mutation will only affect a single gene, and is unlikely to affect global functions or cause apoptosis. Very few genes are only required at specific temperatures (genes required for stress response is an example). It is possible that the gene in question is required only at the restricted temperature, however, this is not the likely cause.

Example Question #1193 : Biology

In 2013, scientists linked a cellular response called the unfolded protein response (UPR) to a series of neurodegenerative diseases, including such major health issues as Parkinson’s and Alzheimer’s Disease. According to their work, the unfolded protein response is a reduction in translation as a result of a series of enzymes that modify a translation initiation factor, eIF2, as below:

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In the above sequence, the unfolded protein sensor binds to unfolded protein, such as the pathogenic amyloid-beta found in the brains of Alzheimer’s Disease patients. This sensor then phosphorylates PERK, or protein kinase RNA-like endoplasmic reticulum kinase. This leads to downstream effects on eIF2, inhibition of which represses translation. It is thought that symptoms of neurodegenerative disease may be a result of this reduced translation.

Which of the following is true of the process of translation discussed in the passage?

Possible Answers:

rRNA is an important component of the translation machinery

Translation takes place in the nucleus

Translation relies on membrane-bound ribosomes

Translation converts rRNA into protein

Translation relies on anticodons in mRNA to bind to codons on tRNA

Correct answer:

rRNA is an important component of the translation machinery

Explanation:

rRNA is an important building block of ribosomes, which synthesize proteins.

The anticodons of tRNA (not mRNA) bind to codons of mRNA (not rRNA), allowing ribosomes to tie together amino acids shuttled in on tRNA molecules. Many ribosomes are bound to the rough endoplasmic reticulum, but are not commonly bound to the membrane of the cell.

Example Question #153 : Cell Biology, Molecular Biology, And Genetics

A surface protein would most likely be translated from mRNA by the __________.

Possible Answers:

Golgi apparatus

cytoplasmic ribosomes

nucleolus

smooth endoplasmic reticulum

rough endoplasmic reticulum

Correct answer:

rough endoplasmic reticulum

Explanation:

Proteins are translated from mRNA by ribosomes. Ribosomes are located on the surface of the rough endoplasmic reticulum, as well as in the cytoplasm. Inter-membrane proteins are constructed by the rough endoplasmic reticulum and sent off in a vesicle, which later becomes part of the cell surface. Generally, proteins created by cytoplasmic ribosomes are destined to serve as cytoplasmic proteins.

Example Question #1221 : Mcat Biological Sciences

Each of the following membrane transport processes requires the use of specific proteins that allow for movement across the plasma membrane EXCEPT __________.

Possible Answers:

facilitated transport via channels

facilitated transport via permeases

simple diffusion

secondary active transport

primary active transport

Correct answer:

simple diffusion

Explanation:

Plasma membranes of the cell are permeable to molecules that pass through the phospholipid bilayer easily, namely small nonpolar molecules. Due to this specificity in permeability, membrane proteins are often required to transport molecules across the bilayer. Simple diffusion occurs when a substance passes through a membrane without the aid of an intermediary. All forms of facilitated transport, along with active transport, require the aid of specific membrane proteins. Thus, simple diffusion is the correct answer.

Example Question #1222 : Mcat Biological Sciences

The cell is the most basic functional unit of life.  Everything that we consider to be living is made up of cells, and while there are different kinds of cells, they all have some essential features that link them all together under the category of "life."  One of the most important parts of a cell is the membrane that surrounds it, seperating it from the rest of the environment.

While organisms from the three main domains live in incredibly different environments, they all possess similar cell membranes.  This phospholipid bilayer protects the cell, giving it a way to allow certain things in while keeping other things out.  Though organisms from different domains have different kinds of fatty linkages in their membranes, they all serve this essential purpose.

Membranes contain all kinds of essential proteins and signal molecules that allow the inside of the cell to respond to the outside of the cell.  In a multicellular eukaryote, this ability can be used to allow cells to communicate.  In a bacterial colony, an extracellular signal could be used to signal other bacteria.  Signals cascade through a series of molecular pathways that go from the outside of the cell all the way to the nucleus and back out again, giving the cell control on a genetic level.  This allows cellular responses to be quick and effective, and it also allows the cell to control how long it stays in that state.

One of the most important membrane proteins is the sodium-potassium pump. What would happen to a cell if this pump suddenly stopped working?

Possible Answers:

The environment would become hypertonic and the cell would shrivel.

Nothing, the cell would be fine.

The environment would become hypotonic and the cell would lyse.

The environment would become hypotonic and the cell would shrivel.

The environment would become hypertonic and the cell would lyse.

Correct answer:

The environment would become hypotonic and the cell would lyse.

Explanation:

The sodium-potassium pump serves to move three sodium ions out of the cell and two potassium ion into the cell.  These ions both have a plus one charge, so when the pump functions, it creates an environment where there are more solutes on the outside of the cell.  if it stopped working, the cell would stop moving sodium out, and since it is a polar molecule, it can't cross the cell membrane on its own.  There would be more solutes inside the cell than on the outside, and water would flow into the cell towards the higher solute concentration, causing the cell to swell and lyse.

Example Question #1223 : Mcat Biological Sciences

The sodium-potassium pump helps to maintain electrolyte gradients through use of ATP. Which of the following best describes this type of transport?

Possible Answers:

Active transport

Filtration

Osmosis

Facilitated diffusion

Diffusion

Correct answer:

Active transport

Explanation:

Active transport most correctly describes this type of movement, as it uses ATP as an energy source. In contrast, the other four choices are all different types of passive transport, constituting types of movement where no energy source is needed. Diffusion is simply the net movement of particles down their concentration gradient. Facilitated diffusion is a similar concept, but uses specialized transport proteins. Osmosis describes the movement of water, and lastly, filtration is the movement of both solute and water molecules.

Example Question #1224 : Mcat Biological Sciences

Assume that there are thirty sodium ions outside the cell and twenty potassium ions inside the cell. What will happen after one cycle of the sodium-potassium pump?

Possible Answers:

There will be 27 sodium ions outside the cell and 18 potassium ions inside the cell

There will be 28 sodium ions outside the cell and 17 potassium ions inside the cell

There will be 32 sodium ions outside the cell and 23 potassium ions inside the cell

There will be 33 sodium ions outside the cell and 22 potassium ions inside the cell

Correct answer:

There will be 33 sodium ions outside the cell and 22 potassium ions inside the cell

Explanation:

To answer this question you need to know the directionality of the sodium-potassium pump and the number of ions pumped each cycle. Remember that each cycle of the sodium-potassium pump moves three sodium ions to the outside of the cell and two potassium ions to the inside of the cell. The amount of sodium ions outside the cell will increase by three and the amount of potassium ions inside the cell will increase by two.

The final result after one cycle of the sodium-potassium pump will be 33 sodium ions outside the cell and 22 potassium ions inside the cell.

Example Question #1225 : Mcat Biological Sciences

Why does the sodium-potassium pump require ATP to function properly?

Possible Answers:

The concentration of sodium ions is greater outside the cell and the concentration of potassium ions is greater inside the cell

The concentration of sodium and potassium ions is greater outside the cell

The concentration of sodium and potassium ions is greater inside the cell

The concentration of sodium ions is greater inside the cell and the concentration of potassium ions is greater outside the cell

Correct answer:

The concentration of sodium ions is greater outside the cell and the concentration of potassium ions is greater inside the cell

Explanation:

When a membrane channel, such as the sodium-potassium pump, requires energy (ATP) to transport molecules it means that the channel is moving molecules against their concentration gradient. This mode of transport is called active transport.

Recall that the sodium-potassium pump moves three sodium ions out of the cell and two potassium ions into the cell per cycle. Since it uses active transport, the sodium-potassium pump must move both sodium and potassium ions against their respective concentration gradients. This means that the concentration of sodium ions is greater outside the cell and the concentration of potassium ions is greater inside the cell.

Note that symporters exist in which facilitated diffusion of one ion is used to pull a second ion against its concentration gradient without the use of ATP. In this manner, ATP is not always necessary to transport an ion against its concentration gradient. When both ions are moving against their gradients, however, or when only one ion is being transported, ATP will be needed.

Example Question #1 : Cellular Structures And Organelles

Which of the following is true about the sodium-potassium pump?

Possible Answers:

It is an antiporter because it transports sodium ions to the outside of the cell and potassium ions to the inside of the cell

It is a symporter because it transports sodium ions to the inside of the cell and potassium ions to the outside of the cell

It is an antiporter because it transports sodium ions to the inside of the cell and potassium ions to the outside of the cell

It is a symporter because it transports sodium ions to the outside of the cell and potassium ions to the inside of the cell

Correct answer:

It is an antiporter because it transports sodium ions to the outside of the cell and potassium ions to the inside of the cell

Explanation:

Antiporters are proteins that carry molecules in opposite directions, whereas symporters are proteins that carry molecules in the same direction. The sodium-potassium pump transports sodium ions out of the cell and potassium ions into the cell. The movement of ions occurs in opposite directions; therefore, the sodium-potassium pump is classified as an antiporter.

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