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Example Questions
Example Question #32 : Plasma Membrane And Transport
An erythrocyte is placed in a solution of 0.9% sodium ions. Erythrocytes contain a concentration of approximately 0.9% sodium chloride. This is a __________ solution, relative to the cell, and the cell will __________.
hypotonic . . . lyse
isotonic . . . swell
hypertonic . . . crenate
isotonic . . . remain the same
hypotonic . . . lyse
The trick to understanding this question is to remember that the sodium chloride actually consists of equal parts sodium ions and chlorine ions; therefore, the internal concentration of solute inside the red blood cell is actually twice the concentration of solute outside the cell. Since the cell contains a higher solute concentration compared to the extracellular environment, the solution is considered hypotonic. Water will flow toward the more concentrated region via osmosis, entering the cell and causing it to swell. Eventually, the swelling can cause the cell to lyse.
Example Question #33 : Plasma Membrane And Transport
What is the function of cholestrol in the cell plasma membrane?
Allow movement of charged particles through the membrane
Promote cell-to-cell recognition
Promote membrane fluidity
Transport molecules across the membrane
Provide ATP
Promote membrane fluidity
The major purpose of cholestrol in the plasma membrane is to maintain membrane fluidity. Carbohydrates and glycoproteins function in cell-to-cell recognition, and proteins function in the transport of particles through the membrane. Charged particles can't freely pass through the membrane unless it is through a carrier protein.
Example Question #34 : Plasma Membrane And Transport
Cells can acquire substances from the extracellular environment through a process called endocytosis, of which there are multiple subtypes.
Which of the following describes the process by which extracellular fluid is taken up by small invaginations of the cell membrane?
Receptor-mediated endocytosis
Pinocytosis
Phagocytosis
Clathrin-mediated endocytosis
Pinocytosis
Pinocytosis, or "cell drinking", refers to the process by which extracellular fluids are engulfed by invaginations of the cellular membrane to create vesicles of the fluid. Phagocytosis is a similar mechanism that acquires extracellular solids into the cell. Receptor-mediated endocytosis occurs when hormones and nutrients bind to a ligand-specific receptor on the cellular membrane and clathrin-mediated endocytosis involves entrance to the cell via clathrin-coated pits.
Example Question #39 : Cellular Structures And Organelles
What is the name for cellular intake of fluid and small solutes?
Pinocytosis
Phagocytosis
Receptor-mediated endocytosis
Endocytosis
Pinocytosis
The best answer would be pinocytosis, or "cell-drinking". Pinocytosis is a specific type of endocytosis; while pinocytosis refers to the uptake of fluids, endocytosis refers to any process involving cellular uptake via vesicles.
Phagocytosis is cellular uptake of large molecules or pathogens, and receptor-mediated endocytosis requires a particular ligand to initiate endocytosis.
Example Question #1261 : Mcat Biological Sciences
Engulfment of a foreign pathogen is an example of ___________ and engulfment of extracellular fluid is an example of ___________; both are forms of ___________.
pinocytosis . . . phagocytosis . . . exocytosis
phagocytosis . . . phagocytosis . . . exocytosis
pinocytosis . . . pinocytosis . . . endocytosis
phagocytosis . . . pinocytosis . . . endocytosis
phagocytosis . . . pinocytosis . . . endocytosis
Recall that endocytosis is the process by which solid particles and fluid and transported from the outside to the inside of the cell by the use of vesicles.
When a cell engulfs a foreign pathogen, it creates a vesicle known as a phagosome. The phagosome carries the pathogen within the cell and fuses with a lysosome, which allows hydrolytic enzymes to digest the pathogen. The process of engulfing the pathogen in a vesicle is known as phagocytosis.
A cell can also form a vesicle around fluids in the extracellular space, via the process of pinocytosis. This vesicle can also fuse with lysosomes, allowing the breakdown of small particulates in the vesicle, which can then be used for energy.
Both phagocytosis and pinocytosis are forms of endocytosis.
Example Question #42 : Cellular Structures And Organelles
ATP synthase is an enzyme that synthesizes ATP in the mitochondrion. Based on this information, which of the following is true regarding cellular transport and ATP synthase?
ATP synthase is irrelevant for both endocytosis and exocytosis
ATP synthase is important for endocytosis only
ATP synthase is important for exocytosis only
ATP synthase is important for both endocytosis and exocytosis
ATP synthase is important for both endocytosis and exocytosis
ATP synthase is an important enzyme that is required for oxidative phosphorylation, a process that produces majority of the ATP in a cell. Both exocytosis and endocytosis are active processes. This means that they require energy (ATP) input; therefore, ATP synthase is essential for both endocytosis and exocytosis.
Example Question #1262 : Mcat Biological Sciences
A protein synthesized inside the cell does not undergo exocytosis. Which of the following is most likely true of this protein?
The protein was synthesized by a free-floating ribosome
The protein was synthesized by the ribosomes on rough endoplasmic reticulum
The protein was synthesized by the ribosomes on smooth endoplasmic reticulum
The protein was synthesized by the ribosomes on the nucleus
The protein was synthesized by a free-floating ribosome
All proteins are synthesized by ribosomes, but the location of the ribosome can vary depending on the function and destination of the protein.
Ribosomes can be found as either as free-floating organelles in the cytosol or as part of the rough endoplasmic reticulum. The proteins synthesized on the rough endoplasmic reticulum will undergo modification and vesicular packaging, and are often destined for regions outside of the cell. These proteins can be expelled from the cell via exocytosis.
On the other hand, free-floating ribosomes in the cytosol produce proteins that are destined to remain in the cytosol. These proteins, once synthesized, are transported within the cytosol and never make contact with the cell exterior. The proteins from free-floating ribosomes do not undergo exocytosis.
Example Question #1263 : Mcat Biological Sciences
Proteins synthesized by ribosomes are processed and packaged in endoplasmic reticulum and Golgi apparatus, respectively. Packaged vesicles are then transported to their target location. Which of the following processes will these proteins most likely experience?
Exocytosis, because these proteins are destined for the cytosol
Exocytosis, because these proteins are destined for the extracellular space
Endocytosis, because these proteins are destined for the extracellular space
Endocytosis, because these proteins are destined for the cytosol
Exocytosis, because these proteins are destined for the extracellular space
Exocytosis is the process by which materials and fluid are transported from the inside of the cell to the outside of the cell by the use of vesicles. Endocytosis is the process by which materials and fluid are transported from the outside to the inside of the cell by the use of vesicles. The question suggests that the proteins are synthesized inside the cell and then packaged into vesicles.
This process is common for a few different types of proteins. Membrane proteins will be integrated into these vesicles and introduced to the cell membrane by fusion of the vesicle. Some proteins destined for the lumens of cell organelles will also be packaged into vesicles, which will fuse with organelle membranes to deposit their contents. Most commonly, however, vesicles will fuse with the cell membrane and release protein contents into the extracellular space via exocytosis.
Golgi apparatus vesicles will never be involved in endocytosis. Of the two options involving exocytosis, only one offers the correct explanation (deposition of proteins into the extracellular space).
Example Question #41 : Cellular Structures And Organelles
Prions are the suspected cause of a wide variety of neurodegenerative diseases in mammals. According to prevailing theory, prions are infectious particles made only of protein and found in high concentrations in the brains of infected animals. All mammals produce normal prion protein, PrPC, a transmembrane protein whose function remains unclear.
Infectious prions, PrPRes, induce conformational changes in the existing PrPC proteins according to the following reaction:
PrPC + PrPRes → PrPRes + PrPRes
The PrPRes is then suspected to accumulate in the nervous tissue of infected patients and cause disease. This model of transmission generates replicated proteins, but does so bypassing the standard model of the central dogma of molecular biology. Transcription and translation apparently do not play a role in this replication process.
This theory is a major departure from previously established biological dogma. A scientist decides to test the protein-only theory of prion propagation. He establishes his experiment as follows:
Homogenized brain matter of infected rabbits is injected into the brains of healthy rabbits, as per the following table:
Rabbit 1 and 2: injected with normal saline on days 1 and 2
The above trials serve as controls.
Rabbit 3 and 4: injected with homogenized brain matter on days 1 and 2
The above trials use unmodified brain matter.
Rabbit 5 and 6: injected with irradiated homogenized brain matter on days 1 and 2
The above trials use brain matter that has been irradiated to destroy nucleic acids in the homogenate.
Rabbit 7 and 8: injected with protein-free centrifuged homogenized brain matter on days 1 and 2
The above trials use brain matter that has been centrifuged to generate a protein-free homogenate and a protein-rich homogenate based on molecular weight.
Rabbit 9 and 10: injected with boiled homogenized brain matter on days 1 and 2
The above trials use brain matter that have been boiled to destroy any bacterial contaminants in the homogenate.
Another experiment shows that PrPC reacts with hormones that circulate among nervous tissue. As a transmembrane protein, what kinds of hormones are most likely to interact with PrPC?
I. Peptide hormones
II. Catecholamines
III. Steroid Hormones
I and III
I and II, only
I, II, and III
II, only
III, only
I and II, only
Students should know that peptide hormones (and catecholamines, but this is not required to answer the question correctly as written here) interact with surface receptors and do not freely go through a membrane. They must interact with the transmembrane surface receptors to initiate a signal transduction cascade. In contrast, steroid hormones can bypass the transmembrance protein receptors by freely diffusing across the memberane, due to their small, nonpolar nature. In this case, only peptide hormones and catecholamines will require the facilitated diffusion mechanism provided by a transmembrane protein.
Example Question #45 : Cellular Structures And Organelles
There are two models for the operation of the Golgi apparatus in eukaryotic cells. As it is difficult to visualize the operation of cells at the molecular level in real time, scientists typically rely on static electron micrographs to see the morphology of organelles. As a result, the dynamic operation of these organelles can sometimes be unclear.
Cisternal Maturation Hypothesis
In the cisternal maturation hypothesis, the cisternae of the Golgi apparatus evolve. Proteins leave the endoplasmic reticulum, and enter the cis-Golgi. The cisterna of the cis-Golgi then matures, with its enzymatic contents and internal environment changing as it becomes the medial-Golgi, and, eventually, the trans-Golgi.
In this model, the proteins never physically leave their membrane-bound cisternae during their transit across the Golgi. Instead, the entire unit of contents remains within the evolving cisternae.
Vesicular Transport Hypothesis
In contrast to the cisternal maturation hypothesis, the vesicular transport hypothesis posits that the cis-, medial-, and trans-Golgi cisternae are more static structures. Instead of evolving around their contents, the contents are physically shuttled via vesicular intermediates from each cisterna to the next.
In the case of vesicular transport, vesicles are shuttled along microtubules. Motor proteins facilitate this movement, with unique proteins being used for each direction of movement along a microtubule.
The Golgi is involved in the packaging of many neuronal transmembrane proteins, including membrane ion channels. Which of the following is true of a normal resting membrane potential?
(Note: membrane potential is typically measured relative to the cytosolic face.)
It is somewhat negative, due to a relatively high permeability via sodium channels
It is somewhat positive, due to a relatively high permeability via sodium channels
It is somewhat negative, due to a relatively high permeability via potassium carriers
It is somewhat negative, due to a relatively high permeability via potassium channels
It is somewhat positive, due to a relatively high permeability via potassium channels
It is somewhat negative, due to a relatively high permeability via potassium channels
Compared to other compounds, the resting permeability of the cell membrane to potassium ions is quite high in most cells. During normal cell metabolism, the sodium-potassium pump moves three sodium ions out of the cytosol, and two potassium ions into the cytosol. The result is a high cytosolic potassium concentration, and an efflux of potassium through the permeable membrane.
It is important to note that the resting membrane potential is set by ion channels, and not ion carriers. The latter will change conformation during translocation of ions, while channels are simply conduits.
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