All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #1 : Differentiation
Homeobox (Hox) genes are essential regulators of development of an organism, as they define region specific development of an embryo along its anterior-posterioir axis. A mutation in the gene Antennapedia, for example, causes Drosophila melanogaster to grow legs from its head instead of antennae.
Given that hox genes regulate segmental identity of an organism, which of the following phenotypes would possibly be present in a fruit fly with a mutation in a Hox gene required for formation of very anterior structures?
Extra wing appendages
Head missing or malformed
Extra legs extending from thorax
Missing wing appendages
Genital structures missing
Head missing or malformed
The only 'very anterior' structure listed among the answers is the head. If this particular hox gene is required to create the proper anterior appendage, we can predict that of the structures listed the head is most likely to be affected.
Example Question #1 : Differentiation
HOX genes are a group of genes that have been well characterized to control body plan development along the anterior to posterior axis in developing embryos. What do HOX genes encode?
Structural proteins
Transmembrane receptors
Golgi-endoplasmic reticulum trafficking proteins
Transcription factors
Mitochondrial proteins
Transcription factors
The correct answer is transcription factors. HOX transcription factors turn on genes during embryonic development to determine the type of segment structure (examples are legs or antennae) at different spatial regions of the embryo. Absence or mis-expression of HOX genes in early development renders misinformed or non-viable organisms.
Example Question #141 : Cell Biology
Which of the following is an example of a multipotent stem cell differentiation into a terminal cell type?
Endoderm cells giving rise to enterocytes
A zygote giving rise to germ layers
Bone marrow cells giving rise to lymphosites
iPS cells giving rise to cardiomyocytes
Bone marrow cells giving rise to lymphosites
Multipotent cell types can give rise to a small number of cell types but have a restricted fate. This is in contrast to totipotent (zygote) or pluripotent cells (germ layers, iPS (induced pluripotent cells)) which can give rise to many different cell types, some that may make up very different parts of the body.
Example Question #141 : Cell Biology
During asymmetric cell division, cells divide such that the mitotic spindles are oriented perpendicular to the plane on which the cells reside. What types of cells are the original parent cells and how does this affect cell lineage?
Asymmetric cell division begins the differentiation process from "parent" stem cells
Asymmetric cell division is proliferation process "parent" stem cells
Asymmetric cell division begins the de-differentiation process from "parent" differentiated cells
Asymmetric cell division is proliferation from "parent" differentiated cells
Asymmetric cell division begins the differentiation process from "parent" immune cells
Asymmetric cell division begins the differentiation process from "parent" stem cells
The correct answer is that asymmetric cell division begins the differentiation process from "parent" stem cells. Symmetric cell division generates two identical daughter cells that have mitotic spindles oriented parallel to the plane on which the cells reside. However, cells that begin a differentiation lineage undergo asymmetric cell division. The perpendicularity of the mitotic spindle in cells that eventually differentiate contributes to this process.
Example Question #1 : Help With Stem Cells
Which is the following answers best describes the key difference between totipotent and pluripotent stem cells?
Pluripotent stem cells can only form a very limited subset of tissue types depending on their origin; totipotent stem cells have the potential to become any of the three embryonic germ layers.
Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism.
Totipotent and pluripotent stem cells are different names for the same cell type, and thus there is no difference in their potential.
Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells only have the potential to become blood and bone cell types.
Pluripotent stem cells have the potential to become an entire organism; totipotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism.
Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism.
Totipotent stem cells are the stem cells with the greatest potential of all stem cell types. A totipotent stem cell can give rise to any embryonic cell type, and can ultimately form an entire organism. Pluripotent stem cells can become any of the three embryonic germ layers, however, they do not have the capacity to form an entire organism because they cannot give rise to the extraembryonic tissue required for development, such as the placenta.
Example Question #1 : Help With Growth Factors
Which of the following mutations in the EGF pathway could lead to increased proliferation of cells?
Mutation abolishing kinase activity of RAF
Mutation causing constitutively phosphorylated Erk
Mutation blocking EGF binding to EGFR
Mutation introducing hydrophillic domains in the hydrophobic region of EGFR
Mutation causing constitutively phosphorylated Erk
The phosphorylation of Erk is the final step of the protein cascade of EGF pathway, and phosphorylated Erk enters the nucleus to increase transcription of genes that induce proliferation. If Erk is constitutively active, it will likely lead to higher proliferation rate.
Preventing EGF from binding to EGFR or disrupting EGFR's ability to enter the membrane would abolish EGF pathway activity and reduce proliferation. Likewise, abolishing kinase activity of RAF would terminate the signal transduction and lead to reduced proliferation.
Example Question #2 : Help With Growth Factors
Production of which of the following cytokines is stimulated when the concentration decreases in the circulatory system?
Interleukin 2
Tumor necrosis factor
Interferon type II
Erythropoietin
Transforming growth factor beta 1
Erythropoietin
Erythropoietin, released by the kidney, stimulates the production of red blood cells, which becomes necessary if circulating has decreased. Tumor necrosis factor stimulates systemic inflammation and regulates the immune system. Transforming growth factor beta 1 controls cell growth, proliferation, differentiation and other processes. Interferon type II modulates immune functions. Interleukin 2 also modulates the immune cells.
Example Question #144 : Cell Biology
Which of the following growth factors is primarily produced by the kidney and is essential for the production of red blood cells?
Insulin-like growth factor
Angiopoietin
Erythropoietin
Fibroblast growth factor
Myostatin
Erythropoietin
Erythropoietin is a glycoprotein that is crucial for the production of red blood cells, a process also called "erythropoiesis." Each of the other answers contains a growth factor, but none of these have a primary function in red blood cell production.
Example Question #141 : Cell Biology
Which of the following cells is not correctly matched to the proper classification of cell (permanent, stable, labile)?
Bone marrow - labile
Cardiac muscle - permanent
Neuron - labile
Hepatocytes - stable
Neuron - labile
Permanent cells remain in G0 and do not divide. They regenerate from stem cells, but cannot undergo division themselves. Neurons, skeletal muscle, cardiac muscle, and red blood cells are permanent cells.
Stable (quiescent) cells enter G1 from G0 when stimulated by introduction of the protein cyclin. Hepatocytes and lymphocytes are examples of stable cells.
Labile cells never go into G0 and divide rapidly with a short G1. Bone marrow, gut epithelium, skin, and hair follicles are examples of labile cells.
Example Question #142 : Cell Biology
Activation of the notch receptor by its ligand, delta, in neighboring cell populations defines patterns of distinct differentiated cell types among cells that have the potential to adopt the same fate. What is this process known as?
Morphallaxis
Invagination
None of these
Lateral inhibition
Delamination
Lateral inhibition
The correct answer is lateral inhibition. Also known as lateral specification, is a process that defines distinct cell types. This process is particularly important in vertebrate embryo nervous system development. Notch signaling is important for many developmental processes. Typically, lateral inhibition involves signaling through the notch receptor, which is stimulated by neighbor cells expressing the notch ligand, delta. Given that only neighboring cells can activate this signaling pathway, defined neighborhoods of undergo differentiation to become a distinct cell type. Morphollaxis is a term that describes regeneration of limbs without cell proliferation. Delamination describes mitotically dividing cells that split one cellular layer into two parallel layers. Furthermore, in terms of development, invagination is the initial step of gastrulation that reorganizes the embryo into a multi-layered organism with a body cavity.