All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #2 : Help With Epigenetics
Histones are proteins that bind to and order the DNA into tight clusters, making it inaccessible to transcription machinery. How does the cells loosen the interaction of the histone and DNA.
By removing acyl groups from the histone
By methylating the DNA
By adding acetyl groups to the DNA
By adding methyl groups to the histone
By adding acetyl groups to the histone
By adding acetyl groups to the histone
By adding acetyl groups to the positively charged histone tails, the cell can loosen the binding of DNA to a histone since DNA is largely negatively charged. This DNA can now be accessed by transcription machinery such as RNA polymerase. In this way acetylation of histones promotes transcription of genes on that piece of DNA. Deacetylation does the opposite - replaces positive charges onto histones so that they can interact more tightly with the DNA, ultimately suppressing expression of the DNA.
Example Question #1 : Help With Epigenetics
Bees use enzymes called DNMT1 and DNMT3 (DNA methyltransferases) to create epigenetic changes that differentiate social castes at a common site pattern. What is this type of epigenetic change called?
Histone modification
None of these are correct
Lysine acetylation
ApT methylation
CpG methylation
CpG methylation
CpG (cytosine-phosphate-guanine) methylation is a common type of epigenetic change caused by this set of enzymes. In this case, the bees use this to create difference castes of bees out of the same shared genome.
Example Question #521 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
Which comparisons between eukaryotic and prokaryotic genomes are true?
Prokaryotic genomes frequently have many transposable elements
Both types of genomes have large spacer regions between genes
Both genomes are replicated in the nucleus
Eukaryotic genes are often single and not in operons like prokaryotic genes
All of these
Eukaryotic genes are often single and not in operons like prokaryotic genes
The only true comparison of those listed is that eukaryotic genes are not often present in operons, like prokaryotes often have (think the frequently studied lac operon). Eukaryotes, not prokaryotes, have many transposable elements (a contributing factor to why our genomes are so large). Prokaryotes do not have large spacer regions between their genes, their genomes are often extremely compact. Prokaryotic cells lack nuclei, thus, DNA replication occurs in the cytosol.
Example Question #521 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
The Frederick Griffith experiment showed that previously safe pneumococcus can become lethal when it incorporates DNA from the surroundings into its genome.
What type of genetic transfer was displayed in the Frederick Griffiith experiment?
Transformation
Transduction
Conjugation
Binary fission
Transformation
When a bacterium has incorporated DNA from the outside environment into its own genome, the process of transformation has occurred.
Example Question #2 : Prokaryotic Genome
Which type of bacterial genetic recombination involves the use of a viral vector?
Assimilation
Conjugation
Transduction
Transformation
Transduction
Sometimes a bacteriophage can encapsulate host bacterial DNA instead of viral DNA. When this virus infects another bacterium, it will inject the previous bacterium's DNA into the new bacterium. This process is referred to as transduction.
Example Question #522 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
What plasmid, usually in multiple copies in a prokaryote, is responsible for production of proteins to attack susceptible organisms?
F plasmids
Virulence plasmids
Col plasmids
R plasmids
Degradative plasmids
Col plasmids
The correct answer is the Col plasmid, which produce attack proteins called colicins. These are generally small and in many copies in a prokaryote for efficiency. F plasmids are involved in conjugation, R plasmids in resistance, degradative plasmids in digestion of unusual substances, and virulence plasmids in the conversion of certain bacteria into pathogens.
Example Question #523 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
What type of phages facilitate specialized transduction?
Lysogenic phages
Animal viruses
Lytic phages
None of these
Retroviruses
Lysogenic phages
Specialized transduction occurs when a prophages excises from the host bacterial genome incorrectly and brings some of the bacterial chromosome with it. This DNA then gets packaged into viral particles along with the viral genome and gets inserted into the next bacterium that virus infects. Since only lysogenic phage can become prophage, specialized transduction can only be mediated by lysogenic phage. Retroviruses are enveloped and thus infect animal cells, not bacterial cells.
Example Question #1 : Help With Transduction, Conjugation, And Transformation
Bacteria are capable of "sharing" genetic material that can be advantageous in certain environments. This type of gene transmission is an example of which of the following?
Fission
Transformation
Transduction
Conjugation
Conjugation
In this example, note that the new genes are being received from a donor bacterium. This is only seen in conjugation events.
Example Question #1 : Mutation And Variation
Which of the following is NOT a type of mutation that can occur in DNA?
Silent
Countersense
Nonsense
Missense
Countersense
There are four principle types of mutation that can affect DNA. Most of these mutations result from point mutations affecting a single nucleotide residue, though nonsense mutations can be caused by insertions or deletions. Frame shift mutations are solely caused by insertions or deletions.
A silent mutation results from the degeneracy of the genetic code. In a silent mutation a single nucleotide is changed, but the overall translation product is unaffected. This can occur because multiple codons are capable of coding for the same amino acid.
A missense mutation results in the swapping of a single amino acid for another in the final translation product. If the amino acids are similar in character, missense mutations can still result in fully functional proteins. When the changed amino acid lacks characteristics of the original, it can result in protein misfolding and loss of function.
Nonsense mutations result in a premature stop codon, and early termination of the translation process. The final product is a shortened version of the protein, often lacking function.
Frame shift mutations cause a shift in the ribosomal reading frame. Every codon downstream of the mutation will be affected, and the protein will be completely altered. Frame shift mutations often result in premature stop codons.
Countersense is not a form of DNA mutation.
Example Question #1 : Mutation And Variation
Which of the following could cause a frameshift mutation?
Insertions or deletions
Mismatches
Deletions
Insertions
Insertions or deletions
Both insertions and deletions are capable of creating frameshift mutations. A frameshift mutation results in a shift in the reading frame of the gene, significantly altering translation. The cause of such a mutation is the insertion or deletion of any sequence of nucleotides within the gene that is not a multiple of three. The following examples detail different types of frameshift mutations.
Normal gene: ATT-CGT-AGG-TAC
Frameshift deletion examples: ATC-GTA-GGT-AC or ATT-CTA-C
Frameshift insertion examples: ATT-TCG-TAG-GTA-C or ACC-CGA-TAG-GTA-C
Mismatches would not cause a shift in the reading frame, like insertions or deletions.