All Genetics Resources
Example Questions
Example Question #7 : Laboratory Procedures And Important Historical Experiments
Choose the correct answer:
The idea that protein could be hereditary material was proved false in 1952 by which two scientists?
Oswald Avery and Frederick Griffith
Alfred Hershey and Martha Chase
James Watson and Francis Crick
Matthew Meselson and Franklin Stahl
Alfred Hershey and Martha Chase
Hershey and Chase used radioisotopes to trace the DNA of a T2 phage (a virus that infects E. coli). Proteins contain sulfur; DNA does not. DNA contains phosphate; proteins do not. Using radioactive forms of sulfur and phosphate, they were able to selectively incorporate these isotopes into either the DNA or protein of a T2 phage and then physically separated the infected and uninfected bacteria. They found that the sulfur was not incorporated, while a portion of the phosphate entered the cells and could be recovered in the next generation. From these results, they were able to conclude that protein was not a hereditary material.
Example Question #8 : Laboratory Procedures And Important Historical Experiments
Choose the correct answer:
Nucleic acid (at the time referred to as "nuclein") was first discovered by whom in 1869?
James Watson
Rosalind Franklin
Charles Darwin
Friedrich Miescher
Friedrich Miescher
Miescher, a Swiss chemist, first identified "nuclein" in the nuclei of white blood cells. He noted that the substance contained higher levels of phosphorus than other proteins and was resistant to proteolysis. This discovery was not widely appreciated for over 50 years.
Example Question #9 : Laboratory Procedures And Important Historical Experiments
Choose the correct answer:
Which of the following is a technique used to measure expression levels of large numbers of genes at the same time by taking advantage of hybridization between two DNA strands?
western blot
northern blot
DNA microarray
PCR
DNA microarray
DNA microarray allows investigators to analyze gene expression on a large scale. A Northern blot only permits analysis of the expression of one (or several) genes at a time. PCR is a method of amplifying DNA and a Western blot allows for analysis of proteins.
Example Question #10 : Laboratory Procedures And Important Historical Experiments
Choose the correct answer:
In the mid-1950s, there were three proposed models for DNA replication: semiconservative, conservative, and dispersive. Which of the following best describes the dispersive replication model?
Two DNA strands separate and each serves as a template for one new DNA strand. Each cell division results in a cell with DNA that is half original, half newly-synthesized.
The whole original DNA double helix is a template for a new double helix. Each cell division results in two cells: one with a new double helix and one with the original double helix.
The original DNA helix breaks into individual nucleotides which reassemble in daughter cells after cell division.
The original DNA double helix is fragmented, with each fragment becoming a template for a new DNA fragment. Each cell division results in two cells with various amounts of old and new DNA.
The original DNA double helix is fragmented, with each fragment becoming a template for a new DNA fragment. Each cell division results in two cells with various amounts of old and new DNA.
The dispersive model (now recognized to be incorrect), proposed that every occurrence of DNA replication would create DNA hybrids (from fragments of the the original double helix) that are one part original and one part new DNA. Each additional round would produce double helices with greater and greater quantities of DNA.
Example Question #121 : Genetics
Choose the correct answer:
Russian biochemist Phoebus Levene is credited with being the first to __________.
discover the carbohydrate component of DNA (deoxyribose)
discover the carbohydrate part of RNA (ribose)
discover the order of the three major parts of a single nucleotide (phosphate, sugar, base)
all of these are correct
all of these are correct
Levene accomplished all of these. He published more than 700 papers during his career. He used hydrolysis to break down and analyze yeast nucleic acids, proposing the composition of nucleic acids (phosphate, sugar, base) in 1919.
Example Question #122 : Genetics
Frederick Griffith’s 1928 experiment involved two different strains of Pneumococcus bacteria. What method of DNA transfer did the experiment demonstrate?
Conjugation
Transformation
Homologous recombination
Transduction
The knocking out of a gene
Transformation
One strain of Pneumococcus was called smooth due to a protective capsule, and the other strain that lacked the capsule was called rough. Mice that were injected with the smooth strain died, and mice injected with the rough strain lived because their immune system killed the bacteria. Griffith also killed some of the smooth strain and found that when mice were injected with the dead smooth strain lived, but mice injected with both live rough strain and dead smooth strain died. He determined that the living smooth strain cells were being transformed by some material from the dead smooth strain. Later experiments proved this material to be DNA.
Example Question #123 : Genetics
Which of the following can be used to separate fragments resulting from transcription of double-stranded DNA?
None of the other answers is correct.
Northern Blot
Southern Blot
Eastern Blot
Western Blot
Northern Blot
The question is asking about separating mRNA fragments, not DNA fragments. Hence, the only procedure applicable is Northern blot. Southern blot is used for DNA, and Western blot is used for protein. In addition, there is no such thing as an Eastern blot.
Example Question #124 : Genetics
The experiment performed by Hershey and Chase demonstrated which important concept?
Law of independent assortment
Protein is the genetic material
Law of segregation
RNA splicing
DNA is the genetic material
DNA is the genetic material
The experiment performed by Hershey and Chase showed that bacteriophages insert their DNA into bacteria, and not their protein. This demonstrated that DNA is the genetic material, and not proteins. Law of independent assortment and segregation are both Mendelian concepts.
Example Question #125 : Genetics
Which of the following individuals is typically considered the "father of genetics" for his important discovers about the rules of genetic inheritance using pea plants?
Frederick Miescher
James Watson
Oswald Avery
Gregor Mendel
Albert Einstein
Gregor Mendel
Gregor Mendel was a German friar who lived in the mid- to late-nineteenth century. He conducted a years-long series of experiments using pea plants in which he carefully tracked each plant's physical traits and determined how cross-fertilization (breeding the pea plant to another pea plant, rather than to itself) affected the plants' offspring. Seed color, flower shape, height, and pod shape were among the traits he studied. In the end, he summarized his work into three laws of heredity: the laws of segregation, independent assortment, and dominance. He also coined the terms "recessive" and "dominant," in reference to genetic traits.
Unfortunately, Mendel's work remained largely unacknowledged through his life. The importance of his studies was only recognized by other scientists decades after his death.
Example Question #1 : Understanding Reproductive Barriers
Two populations of the same species over time grow distant from one another. At what point will these two populations be considered different species?
When there is a physical barrier, such as a river, between the two populations
When the populations begin to eat different foods
When the two populations have not been in contact with one another for two hundred years
When the distance between them exceeds the carrying capacity
When they are no longer able to interbreed
When they are no longer able to interbreed
Speciation is the event that occurs when two populations of a particular species can no longer interbreed. Speciation is not defined by physical barriers or by the time that two populations are separate from one another. In fact, two populations of the same species can be apart any distance or time, and if they can still interbreed they are considered the same species even if they look completely different.
Note that carrying capacity refers to the maximum number of individuals in a population that the natural resources of the surrounding environment can support. It is, essentially, the maximum healthy population size, and is not a measure of distance as implied in the answer choice.