All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #411 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
When modifiying histones with either activating or repressing marks, such as with acetyl and methyl groups, histone acetyltransferases and histone methyltransferases normally modify which amino acid?
Guanine
Alanine
Aspartic acid
Lysine
Phenylalanine
Lysine
Histones are almost always modified on lysines. Acetylation normally is an activating mark, and methylation is normally a repressing mark of chromatin structure.
Note that guanine is not an amino acid, but a nucleotide.
Example Question #411 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
Which of the following is indicative of enhancers in the chromatin landscape of a given cell?
All of the other answers are correct
H3K4me1 and H3K27Ac histone marks
H3K4me1 histone marks
H3K4me1 and H3K27me3 histone marks
DNase1 hypersensitive sites
All of the other answers are correct
The correct answer is all of the other answers are correct. H3K4me1 (Histone 3 Lysine 4 methyl 1) marks where enhancers are in the chromatin landscape. By further identifying H3K27Ac or H3K27Me3 marks on the same histone, we can determine whether the enhancer is active or inactive, respectively. A DNase 1 hypersensitivity assay will preferentially cut open chromatin, which often is indicative of enhancer regions in the chromatin landscape.
Example Question #411 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
If the recombination frequency between two genes is __________ then the genes are most likely __________.
high . . . far apart
zero . . . far apart
low . . . far apart
high . . . linked
high . . . far apart
Recombination frequencies are used to map genes on chromosomes by determining their relative distances from other genes. If a distance is large, there is a higher chance that a recombination event can occur between these two genes. Linkage occurs when genes are so close to one another that the genes always segregate together (recombination never occurs between them). The only answer that makes sense is that high recombination frequencies lead to the conclusion that two genes are far apart.
A low recombination frequency would indicate that the gene loci are close together, and a recombination frequency of zero would indicate linked genes.
Example Question #1 : Genomics
A scientist performs a series of experiments to determine the recombination frequencies between the following genes. He acquires the following data:
W-X: 3%
X-Y: 2%
Y-Z: 13%
Z-W: 8%
Which of the following choices places the genes in the correct order relative to one another?
W, Y, Z, X
X, W, Z, Y
W, X, Y, Z
Z, W, X, Y
Z, W, X, Y
The larger the recombination frequency, the larger the distance between two genes. By looking at the data, we know that genes W and X are close to one another. Also, genes X and Y are close to one another. Gene Z, however, seems to be far away from both W and Y (but closer to W). We can represent these distances relatively in a picture:
W - - - X (3)
X - - Y (2)
Y - - - - - - - - - - - - - Z (13)
Z - - - - - - - - W (8)
The most likely explanation is that W, X, and Y are close to one another and Z is located slightly farther away on whichever side W is closest. A spatial map would look something like this:
Z - - - - - - - - - - W - - - X - - Y
Example Question #2 : Genomics
If two genes are found to have a recombination frequency of 25%, what does this mean about the location of the two genes?
The genes are located on the same chromosome and neither extremely close, nor extremely far apart
The genes are located on the same chromosome and are on opposite ends of the chromosome
The genes are on different chromosomes
The genes are linked
The genes are located on the same chromosome and neither extremely close, nor extremely far apart
If the genes were linked, there would be an incredibly small recombination frequency. If the genes were on opposite ends of the chromosome or on separate chromosomes, the recombination frequency would approach the maximum of 50%.
Because the recombination frequency is relatively intermediate, we can conclude that the distance between the genes does not fall at either extreme. The genes are neither very close, nor very far apart.
Example Question #412 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
What does it mean to say that two genes are linked?
The genes share a promoter
The genes code for the same mRNA
The genes almost always segregate together during meiosis
The genes overlap one another
The genes almost always segregate together during meiosis
Genetic linkages are determined by frequencies of recombination. These are measures of how often chromosomal crossovers will take place between two genes. The closer the loci of the two genes are on a chromosome, the less likely a crossover event will separate the two genes. If the recombination frequencies are sufficiently low, the genes are considered to be linked.
Genetic linkage has nothing to do with genes coding for the same mRNA, sharing a promoter, or overlapping one another. Linked genes still code for distinctly separate traits/proteins and have different loci (don't overlap).
Example Question #413 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
Which of the following is true of gene families?
I. They likely arose from gene duplications
II. They are only seen in eukaryotic genomes
III. Members of the same family have identical DNA and amino acid sequences
I and II
II and III
I only
I, II, and III
I only
Gene families consist of several copies of genes that encode very similar proteins. These likely arose due to gene duplications, and were altered by mutation over time to generate separate similar proteins. It is not a requirement that members of gene families have identical DNA or amino acid sequences. Both prokaryotes and eukaryotes have gene families.
A common example is the homeobox, or Hox, gene family, which codes for several proteins that are essential for developmental timing and orientation.
Example Question #1 : Genomics
Which of the following is not true about transposable elements?
Transposable elements can cause disease
Transposable elements are primarily considered non-coding DNA
Transposable elements often move around the genome
Transposable elements are only found in eukaryotes
Transposable elements are only found in eukaryotes
Transposable elements are portions of the DNA that are free to move around the genome and are generally considered non-coding DNA. This can be potentially dangerous, however. Transposable elements can insert themselves in the coding regions of genes, thus making them non-functional. This can lead to disease. Both eukaryotic and prokaryotic genomes contain transposable elements.
Example Question #1 : Help With Transposable And Repeated Elements
Transposable elements, or transposons, are separated into two classes. Which of these categories of life have class I transposons in their genomes?
I. Bacteria
II. Yeast
III. Eukaryotes
None of these
III only
II and III
I and II
I, II, and III
II and III
Class I transposable elements are RNA-mediated elements of a single evolutionary origin, and are found in yeast, which only have class I elements, and in eukaryotes, which have both class I and class II elements. Bacteria only have class II elements, and hence are not included in the correct answer to this question.
Example Question #2 : Help With Transposable And Repeated Elements
What differentiates a LTR retrotransposon and a retrovirus?
None of these are correct
Retroviruses do not insert DNA into their host
LTR retrotransposons cannot move between organisms
Retroviruses are the only ones present in eukaryotes
Retroviruses encode an envelope protein
Retroviruses encode an envelope protein
The only difference between most LTR retrotransposons and retroviruses are that retroviruses can encode an envelope protein. Phylogenetic analyses have shown that retrotransposons and retroviruses are extremely closely related, and may be direct ancestors of one another.