All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #1 : Rna, Transcription, And Translation
Which polymerase is involved in transcribing ribosomal RNA (except 5S rRNA)?
DNA polymerase IV
DNA polymerase V
RNA polymerase I
RNA polymerase II
RNA polymerase III
RNA polymerase I
The correct answer is RNA polymerase I. The sole purpose of RNA polymerase I in eukaryotes is to transcribe ribosomal RNA, with the exception of 5S rRNA, which is transcribed by RNA polymerase III. RNA polymerase III also transcribes tRNAs and other small RNAs. Transcripts of RNA polymerase II are 5' capped, polyadenylated, and spliced to ultimately be translated into functional protein. DNA polymerase IV/V are polymerases involved in DNA replication and repair.
Example Question #1 : Rna, Transcription, And Translation
What is the role of the promoter region in the regulation of gene expression?
Allow for alternative splicing and recombination of genetic components
Recruit RNA polymerase and transcription factors to DNA
Alter gene expression by binding transcription factors
Bind negative regulators to inhibit gene expression
Recruit RNA polymerase and transcription factors to DNA
The promoter region is the site of a gene where RNA polymerase and other transcription factors bind to DNA, upstream from the gene locus. A mutation in this region commonly results in a decrease in the amount of gene transcribed.
An enhancer region is a stretch of DNA that alters gene expression by binding transcription factors, while a silencer region is a site on the gene where repressor proteins bind. Introns are intervening non-coding segments of DNA that are not expressed in the final protein. Alternative splicing patterns of introns and exons allows for multiple proteins to be generated from a single gene.
Example Question #2 : Rna, Transcription, And Translation
Which of the following are methods for termination of transcription in prokaryotes?
I. Release factor binding
II. Rho-mediated termination
III. Hairpin loop (stem loop) formation
III only
I, II, and III
I only
II and III
II and III
The binding of release factors is a common way to terminate translation, not transcription.
Rho-mediated termination and hairpin loop formation are both common ways to terminate prokaryotic transcription. The formation of the hairpin loop disrupts the transcription machinery and the DNA-RNA interactions, which allows termination of transcription. Rho is a protein that is capable of binding single-stranded RNA and terminating transcription.
Example Question #11 : Rna, Transcription, And Translation
What event is indicative of transcription initiation?
RNA polymerase binds to the promoter
The RNA strand begins to be synthesized
Telomerase lengthens the telomeres of the DNA
RNA polymerase is removed from the DNA
RNA polymerase binds to the promoter
During the initiation of transcription, RNA polymerase and a group of transcription factors bind to the promoter for a given gene. This DNA segment signals the RNA polymerase where to begin creating the RNA strand.
Example Question #1 : Help With Transcription Processes
Which of the following best describes distant regulatory sequences that influence gene expression?
Transcriptional start sites
Introns
Exons
Promoters
Enhancers
Enhancers
The correct answer is enhancer. Transcription factors and mediators bind enhancer regions of DNA and influence the transcription of distant genes by chromatin looping to the proximal promoter. Promoters are regulatory sequences, however, they are typically 2 kilobase pairs upstream of the gene for which they influence transcription. Introns and exons make up a gene and are the non-coding and coding regions of the gene, respectively. The transcriptional start site consists of the first few nucleotides that are transcribed into an mRNA sequence from a gene, usually containing the 5' untranslated region (UTR).
Example Question #12 : Rna, Transcription, And Translation
When does alternative splicing occur?
Following mRNA translocation to the cytosol
Following translation
Following mRNA polyadenylation
Following pre-mRNA transcription in the nucleus
Following mRNA loading into ribosome
Following pre-mRNA transcription in the nucleus
The correct answer is following pre-mRNA transcription in the nucleus. Pre-mRNA contains introns and exons. Following transcription, splicing and alternative splicing occurs to remove introns and select exons, respectively, by the spliceosome. Following splicing, 3' poly adenylation and 5' capping occur to generate a mature mRNA transcript that will translocate to the cytosol and be translated by ribosomes.
Example Question #1 : Help With Transcription Processes
What element(s) do all eukaryotic promoter regions share?
Enhancers that accept binding agents
Transcription factors
All of these
None of these
A basal promoter (TATA box)
All of these
Eukaryotic promoters share basic, highly conserved structure. This area does not evolve quickly because it is extremely important in DNA transcription. These promoters (in most cases) include a basic basal promoter like a TATA box, and enhancers that bind to transcription factors.
Example Question #2 : Help With Transcription Processes
During transcription of a eukaryotic cell, what determines the template strand?
The base sequence of the operator
The base sequence of the enhancer
The base sequence of the promoter
The location of the MET start codon
The location of the AUG start codon
The base sequence of the promoter
The TATA box is found in the promoter region of the template strand. This TATA box serves as a signal for the initiation of translation of DNA into mRNA. The location of the promoter region and it's unique base sequence signals the start of the translation process.
Example Question #1 : Help With Rna Structure And Modification
Which of the following processes is not a type of RNA processing carried out by eukaryotes?
Polyadenylation of the 3' end
Splicing and removal of introns
Capping of the 5' end
Nucleotide excision repair
Nucleotide excision repair
Processing of pre-mRNA occurs in the nucleus. After transcription, three crucial modification take place. A 7-methylguanosine molecule is added to the 5' end to form a cap. Polyadenylation is added to the 3' end to create a poly-A tail. Introns are spliced out by spliceosomes, removing the non-coding regions of the RNA. The final product after modifications is considered a mature mRNA; prior to this, the transcript is known as heteronuclear RNA (htRNA).
Nucleotide excision repair is a method of proofreading after DNA replication to reduce the frequency of mutation.
Example Question #441 : Gre Subject Test: Biochemistry, Cell, And Molecular Biology
What are exons?
Genetic information coding for an amino acid sequence that will form a functional protein
Intervening non-coding segments of DNA
Site where repressor proteins bind
Set of genes that are adjacent to one another in the genome and are coordinately controlled
Genetic information coding for an amino acid sequence that will form a functional protein
Exons contain the actual genetic information coding for protein. In contrast, introns are intervening non-coding segments of DNA. During the splicing process of pre-mRNA modification, introns are removed from the sequence. Alternative splicing patterns allow multiple exon sequences to be created from a single gene, resulting in multiple proteins.
An operon is a set of genes that are adjacent to one another in the genome and are coordinately controlled. The silencer region is a site of a gene where repressor proteins bind to regulate gene expression.