All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #1 : Cell Biology
Gram positive bacteria have __________ cell wall(s) and Gram negative bacteria have __________ cell wall(s).
two . . . one
two . . . two
one . . . two
one . . . one
one . . . one
Gram stain is a laboratory technique used to distinguish bacteria. Gram positive and Gram negative bacteria are distinguished based on their cell walls. Gram positive bacteria have a single thick cell wall (outside its cell membrane). Upon Gram staining, these bacteria will hold the Gram stain because of their thick cell wall. The Gram negative bacteria also have only one cell wall; however, it is a lot thinner and is sandwiched between two cell membranes. The thin nature of the cell wall makes it easier for the Gram stain to leak out of the bacterial cell.
Example Question #1 : Cellular Structures And Functions
Which of the following is true regarding cell walls?
I. They are only found in bacteria that can be Gram stained
II. Some organisms that have cell walls can undergo photosynthesis
III. They expand when a cell is placed in a hypoosmolar environment
II and III
I, II, and III
I and III
II only
II only
Cell walls are robust structures found in most bacteria, fungi, and plants. Gram stain depends on the properties of the cell wall. Some bacteria don’t stain well with Gram stain (such as Mycobacterium that causes tuberculosis) because of unique cell walls (not lack thereof). Fungal and plant cell walls are also made up of polysaccharides. Fungal cell walls contain a special molecule called chitin whereas plant cell walls contain cellulose. Recall that plants obtain energy from sunlight via photosynthesis; therefore, photosynthetic organisms do contain cell walls.
One of the biggest function of a cell wall is to prevent cellular swelling. Recall that a cell placed in a hypoosmolar solution will swell due to the influx of water; however, this cellular swelling will not occur in a cell containing cell wall because the robust cell wall prevents contents of the cell from expanding, thereby preventing cellular lysis.
Example Question #11 : Cell Biology
How are glucose monomers linked in the cellulose polymers present in a plant cell wall?
Beta (1-6) linkages
Beta (1-4) linkages
Alpha (1-4) linkages
Alpha (1-4) linkages
Beta (1-3) linkages
Beta (1-4) linkages
Cellulose is a polymer of glucose linked by beta (1-4) linkages. Humans lack the enzyme that catalyzes the cleavage of this bond; thus cellulose (dietary fiber) is indigestible and passes through the digestive system unchanged.
Example Question #1 : Help With Membrane Transport
Which of the following can freely diffuse through the cell membrane?
I. Oxygen
II. Potassium ions
III. Glucose
IV. Hydrogen ions
I only
I, II, III, and IV
I, II, and IV
I and III
I only
Only small, nonpolar molecules can freely diffuse through the plasma membrane. From this group, only oxygen fits this description. Hydrogen and potassium ions are charged and, therefore, carry a polar element that cannot diffuse through the membrane. Glucose is too large, and also contains a polar aldehyde group. Oxygen atoms generally contribute to molecular polarity due to their abundance of valence electrons. When arranged symmetrically, however, such as in the oxygen molecule, the oxygen atoms result in zero net polarity.
Transmembrane proteins and channels are used for facilitated diffusion of glucose and small ions.
Example Question #2 : Help With Membrane Transport
Which of the following is false with regard to the LDL-receptor?
Its cargo is low density lipoprotein
Once bound to cargo, it enters the cell via COPII-mediated endocytosis
It releases its cargo in the low pH environment of the endosome
It is shuttled back to the cell surface after it delivers its cargo
Once bound to cargo, it enters the cell via COPII-mediated endocytosis
The LDL-receptor enters the cell via clathrin-mediated endocytosis, not COPII. COPII is involved in budding off of the endoplasmic reticulum.
All of the other given answer options are true.
Example Question #3 : Help With Membrane Transport
Which of the following is false concerning facilitated diffusion?
It requires the use of proteins in the membrane
It moves substances down their electrochemical gradients
It requires energy in order to move substances
It is typically used to move charged or polar substances
It requires energy in order to move substances
Facilitated diffusion is used to move polar or charged substances down their electrochemical gradients. Although this does require the use of integral membrane proteins, no energy is used during this process, as it is still a form of diffusion.
Example Question #4 : Help With Membrane Transport
When will water have a net flow into a cell?
When the cell is in a hypotonic solution
When there is more solute outside of the cell compared to inside the cell
When the cell is in an isotonic solution
When the cell is hypotonic to its surrounding environment
When the cell is in a hypotonic solution
The direction for the diffusion of water, or osmosis, can be predicted by comparing the tonicities of the cell and its surroundings. When the cell has more solute in it than the external environment, water will flow into the cell in order to help even out the concentrations.
As a result, water will flow into the cell when it is found in a hypotonic solution.
Example Question #5 : Help With Membrane Transport
A person is found to have a defect in his ATP synthase gene. Which of the following will be halted in his cells?
More than one of these are true
Sodium-potassium pump because it involves active transport
Potassium channels because it involves active transport
Sodium-potassium pump because it involves passive transport
Sodium-potassium pump because it involves active transport
ATP synthase is a molecule used to generate energy for the cell (in the form of ATP). Lack of ATP synthase will halt many active processes that require energy. Recall that sodium-potassium pump is found on multitude of cells in the body and functions to maintain the intracellular concentration of ions. This pump transports sodium and potassium ions against its concentration gradient (sodium out of and potassium into the cell). Since it is transporting against a concentration gradient, the sodium-potassium pump carries out active transport and, therefore, requires energy. Decreased ATP synthase activity will lead to decreased ATP levels and, subsequently, affect the sodium-potassium pump.
Potassium channels are selective molecules (selective for potassium) that transport potassium ions along its concentration gradient (from inside of cell to outside); therefore, this is a type of passive transport and does not require energy.
Example Question #1 : Help With Membrane Transport
A researcher observes that a molecule is brought inside the cell via membrane transporters. What can the researcher conclude about this type of transport?
Cell expends energy to transport this molecule
The molecule is found in higher concentration outside the cell
None of these can be concluded
Cell does not expend energy to transport this molecule
None of these can be concluded
Membrane transporters are molecules found on plasma membranes that act as gateway for several molecules that enter and exit cells. These transporters are usually specific for one or two molecules and transport them across the cell membranes. The membrane transporters can transport ions/molecules along or against the concentration gradients; therefore, membrane transporters perform both passive and active transport. Transporters that move molecules along concentration gradient carry out a special type of passive transport called facilitated diffusion whereas transporters that move against concentration gradient carry out active transport (requires energy).
The question doesn’t state whether the molecule is brought along or against its concentration gradient; therefore, the membrane transporter could be a facilitated transporter or an active transporter.
Example Question #1 : Help With Membrane Transport
In a normal individual, which of the following molecule(s) always enter(s) the cell via passive transport?
I. Calcium
II. Potassium
III. Sodium
II only
I only
I and II
I and III
I and III
There are two types of membrane transport: passive and active transport. Passive transport involves movement of molecules along its concentration gradient. This is a spontaneous process and does not require energy. Examples of passive transport include simple diffusion of lipophilic molecules and facilitated transport of molecules involving a membrane transporter (such as an ion channel). Active transport, on the other hand, moves molecules against their concentration gradient. This is not a spontaneous process and, therefore, requires energy.
To answer this question we need to recall the relative concentrations of the three ions presented in the question. Potassium ions are found in higher concentrations inside the cell whereas calcium and sodium are found in higher concentrations outside the cell. This means that moving calcium and sodium ions inside the cell will involve moving along their respective concentration gradients (from high to low concentration) whereas moving potassium ions inside the cell will involve moving against the concentration gradient; therefore, sodium and calcium come into the cell via passive transport whereas potassium enters via active transport.