AP Biology : AP Biology

Study concepts, example questions & explanations for AP Biology

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Example Questions

Example Question #2 : Understanding Dna Repair

Which enzyme is not involved in DNA replication?

Possible Answers:

Gyrase

Ligase

DNA polymerase

Lipase

Helicase

Correct answer:

Lipase

Explanation:

Lipase is the general name for an enzyme that breaks down lipids. Ligase joins the Okazaki fragments on the lagging strand of the DNA during replication. DNA polymerase is the enzyme that catalyzes the polymerization of nucleotides in the 5' to 3' direction. Helicase separates the two strands of the double helix to facilitate formation of the replication bubble. Gyrase relieves strain on the DNA while it is being unwound by helicase. 

Example Question #2 : Dna, Rna, And Proteins

Which enzyme has a proofreading ability during DNA replication?

Possible Answers:

DNA helicase

DNA gyrase

Primase

DNA polymerase

Correct answer:

DNA polymerase

Explanation:

Proofreading is an important part of the DNA replication process to ensure that if mismatched base pairs are incorporated into the newly synthesized DNA strands, they get replaced with correct base paired nucleotides. Mismatched base pairs have the potential to cause disease. DNA polymerases have proofreading abilities. They are able to remove mismatched nucleotides from the end of a newly synthesized strand. Post-replication repair mechanisms also exist to prevent damage and error.

Example Question #1 : Dna Repair And Replication

You are trying to perform in vitro DNA replication on a small circular piece of DNA. You have DNA polymerase, Primase, Helicase, DNA ligase and all of their accessory proteins. You can get DNA replication to initiate but it never goes for very long without stopping. You visualize your small piece of DNA under an electron microscope and notice that after the initiation of replication, it looks all knotted up. What enzyme can you add to remedy this problem?

Possible Answers:

Telomerase

Knottase

Reverse Transcipase

RNA polymerase

Topoisomerase

Correct answer:

Topoisomerase

Explanation:

As the replication fork of DNA proceeds and continues to unwind the double helix, the DNA upstream of the fork gets over wound and knotted up which will eventually arrest replication as the fork will not be able to proceed any further. The enzyme topoisomerase corrects for this overwinding ahead of replication forks by swiveling and rejoining DNA strands

Example Question #651 : Ap Biology

Which of the following characteristics is NOT seen in both DNA and RNA?

Possible Answers:

A pentose sugar

Read in the 5'-to-3' direction

A double helix

Adenine

Correct answer:

A double helix

Explanation:

DNA and RNA share many characteristics. They are both composed of nucleotide monomers and are read in the 5'-to-3' direction. They also share the same complementary base pairs, except RNA uses uracil in place of thymine; both contain adenine.

RNA does not present in a double helix structure, and is typically single stranded.

Example Question #1 : Dna, Rna, And Proteins

A section of mRNA is composed of 28% guanine bases. What percentage of the RNA strand is cytosine bases?

Possible Answers:

More information is needed in order to answer this question.

Correct answer:

More information is needed in order to answer this question.

Explanation:

Remember that mRNA is not a double helix like DNA; RNA is only one single strand of nucleotides. This means that we are unable to say that there are just as many cytosine bases as guanine bases, even though they would be able to form nucleotide pairs in DNA. For all we know, there could be zero cytosine bases! More information is needed before we can make a conclusion as to how many cytosine bases are in the RNA section.

Example Question #1 : Dna, Rna, And Proteins

Which of the following statements is correct about the differences between DNA and RNA?

Possible Answers:

RNA contains the same bases as DNA, except uracil is present instead of guanine

The sugar molecule in RNA has one more hydroxyl group than the sugar molecule in DNA

DNA is present as a single-stranded molecule while RNA is double-stranded

DNA is not present in prokaryotes, while RNA is

RNA is synthesized from DNA during transcription, but DNA can never be synthesized from RNA

Correct answer:

The sugar molecule in RNA has one more hydroxyl group than the sugar molecule in DNA

Explanation:

The only correct statement here is the one regarding the types of sugar in the two molecules. RNA stands for "ribonucleic acid," which is a simple way to remember that it contains the sugar ribose. DNA, on the other hand, stands for "deoxyribonucleic acid." Its sugar is deoxyribose, which is identical to ribose except it is missing a hydroxyl (-OH) group on its second carbon. In total, RNA contains three hydroxyl groups, while DNA contains only two.

In RNA, uracil replaces thymine, not guanine. DNA is generally double-stranded and RNA is generally single-stranded (though both can exist in either form). Prokaryotes contain both DNA and RNA. Finally, DNA is transcribed to RNA in most biological organisms, but RNA can be reverse transcribed to DNA by the protein reverse transcriptase, which is found in some viruses.

Example Question #11 : Dna, Rna, And Proteins

Which of the following statements is false?

Possible Answers:

DNA and RNA utilize different sugars in their sugar-phosphate backbones

DNA is a polyanion, while RNA is a not

DNA uses thymine, while RNA uses uracil

DNA is typically double-stranded, while RNA is typically single-stranded

Correct answer:

DNA is a polyanion, while RNA is a not

Explanation:

Both DNA and RNA are polyanions. This is just a fancy way of saying that they are polymers of negatively charged molecules. The phosphate groups in the sugar-phosphate backbone account for this, as phosphate groups generally carry a charge of negative three.

DNA uses thymine and deoxyribose sugar, while RNA uses uracil and ribose sugar. While DNA is usually molded into a double-stranded helix, RNA is usually single-stranded, which allows for the binding of anticodons during translation.

Example Question #2 : Dna And Rna Structure

Which of the following is characteristic of RNA?

Possible Answers:

RNA is typically double-stranded

RNA has a hydroxyl group on the 2’ carbon of ribose

RNA is a permanent molecule in most cells

RNA is less stable than DNA

Correct answer:

RNA has a hydroxyl group on the 2’ carbon of ribose

Explanation:

RNA and DNA are very similar in composition, but differ in structure and function. DNA is used to code for genetic material, while RNA is used to generate protein products. Since DNA has a long-term goal of storing information and RNA has a short-term goal of increasing production, it makes sense that DNA is a permanent molecule and RNA is transient. Soon after translation, mRNA is degraded by ribonuclease (RNase). The transient nature of RNA is also linked to its stability. DNA must be very stable to avoid problems with gene storage. DNA is double-stranded to help enhance stability. In contrast, RNA can afford to be less stable and is easily degraded, partially due to its single-stranded structure.

Another key difference between DNA and RNA is the sugar component of the nucleic acid backbone. DNA uses deoxyribose, which lacks a hydroxyl (-OH) group on the 2' carbon. RNA uses ribose, in which this hydroxyl group is present on the 2' carbon.

Example Question #1 : Understanding Differences Between Dna And Rna

Which of the following is not a true characteristic of an RNA molecule?

Possible Answers:

It can be double stranded

It contains deoxyribose sugar

It can be single stranded

It contains the nucleotide uracil

Correct answer:

It contains deoxyribose sugar

Explanation:

RNA molecules are most commonly found in a single stranded form (e.g. mRNA), but they can also be found in a double stranded form (e.g. viruses). It has four base pairs—uracil, adenine, guanine, and cytosine—and a ribose sugar-phosphate backbone. DNA molecules contin a deoxyribose sugar in their sugar-phosphate backbone; thus, this answer is incorrect.

Example Question #3 : Dna And Rna Structure

Which of the following types of RNA is double stranded?

Possible Answers:

tRNA

rRNA

mRNA

Viral RNA

Correct answer:

Viral RNA

Explanation:

Many types of RNA exist within cells, some are single stranded and others are double stranded. The following are among the types of single stranded RNA: mRNA (messenger RNA), tRNA (transfer RNA), and rRNA (ribosomal RNA). Messenger or mRNA carries genetic information from DNA to ribosomes for protein synthesis. Transfer or tRNA carries amino acids to ribosomes that match with mRNA codons. Last, ribosomal or rRNA codes for ribosomes, which are necessary for protein synthesis. An example of a double stranded RNA molecule is viral RNA. Viral RNA is the genetic material of many viruses and has a structure of two complementary strands.

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