SAT II Biology M : DNA, RNA, and Proteins

Study concepts, example questions & explanations for SAT II Biology M

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Example Questions

Example Question #21 : Molecular Biology

Which of the following most accurately describes the primary structure of a protein?

Possible Answers:

The linear amino acid sequence of the protein

The alpha helicies of the protein

The non-covalent interactions between multiple protein subunits which come together to form a larger protein

The hydrogen bonds between amino acids in the protein

The final three-dimensional structure of the protein

Correct answer:

The linear amino acid sequence of the protein

Explanation:

The primary structure of a protein is simply the linear amino acid sequence from which the protein is made. "Secondary structure" refers to the folding and coiling of this single strand as it interacts with itself, forming hydrogen bonds between amino acids of that strand. Alpha helices and beta-pleated sheets are two different types of secondary structures that can be formed by hydrogen-bonding between amino acids of a protein sequence that has folded over onto itself. "Tertiary structure" refers to the final three-dimensional structure of a single protein subunit. "Quaternary structure" refers to the non-covalent interactions between multiple protein subunits which come together to form a larger protein.

Example Question #1 : Enzymes

Which is an example of a biological catalyst that is not a protein?

Possible Answers:

Lyases

Transferases

Hydrolases

Ribozymes

Spliceosomes

Correct answer:

Ribozymes

Explanation:

This question ultimately hinges on knowing the difference between ribozymes and spliceosomes because transferase, hydrolase, and lyase should all be recognized as proteins that function as enzymes. Transferase catalyzes reactions that facilitate the transfer of functional groups. Hydrolase works to catalyze hydrolysis reactions. Lyase works to catalyze reactions that break down double bonds. Spliceosomes are a unit of proteins and RNA that work to catalyze reactions that splice out introns in RNA to form mature mRNA ready for translation. Ribozymes are important because they also splice RNA into mRNA, but they do not have a protein component to them. The discovery of Ribozymes was a breakthrough in that it was the first evidence that not all enzymes are proteins.

Example Question #1 : Enzymes

Which of the following statements about enzymes is true?

Possible Answers:

Enzymes are useful for slowing down reactions in living things.

Enzymes are lipids.

Enzymes will work at any pH value.

Enzymes work best only at certain temperatures.

Correct answer:

Enzymes work best only at certain temperatures.

Explanation:

Enzymes have an "optimal temperature," or best temperature that they work at. If that temperature is below or above its optimal temperature, the enzyme will decrease in activity; if the temperature change is great enough, the enzyme could even denature (no longer work).

Example Question #2 : Enzymes

Cellular respiration involves a series of chemical reactions. Which of the following is a primary way that enzymes affect these reactions?

Possible Answers:

They decrease the pH of the products

They change the location of the reactions in the cell

They increase the rate of reactions

They take the place of oxygen as a reactant

Correct answer:

They increase the rate of reactions

Explanation:

The questions is asking how enzymes affect reactions. The function of an enzyme is to speed up chemical reactions, which will increase the overall rate of the reaction, thus "increasing the rate of the reaction" is the correct answer. 

Example Question #1 : Enzymes

The role of an enzyme in a chemical reaction is to change which of the following?

Possible Answers:

The temperature at which the reaction occurs

The pH at which the reaction occurs

The type of reaction

The activation energy of the reaction

Correct answer:

The activation energy of the reaction

Explanation:

The function of an enzyme is to speed up chemical reactions. They do this by lowering the activation energy, which is the minimum energy that must be available for a chemical reaction to occur. If the energy required is lowered, the reaction can go faster. Thus the correct answer is an enzyme changes "the activation energy of the reaction."

Example Question #3 : Enzymes

The reactants in an enzyme-catalyzed reaction are known as __________.

Possible Answers:

pHs

active sites

products

substrates

Correct answer:

substrates

Explanation:

A reactant is a substance that undergoes change during a reaction. During an enzyme reaction specifically, the reactant is called the substrate, as a substrate is the substance in which an enzyme acts on and changes. 

Example Question #4 : Enzymes

What can affect the productivity of an enzyme?

Possible Answers:

pH

Temperature

None of these

Both pH and temperature

Correct answer:

Both pH and temperature

Explanation:

Both pH and temperature can affect how productive an enzyme is. If an enzyme is functioning in an environment that is not at its optimal pH or optimal temperature, the enzyme's activity will decrease. 

Example Question #5 : Enzymes

Why are enzymes necessary for most cellular reactions?

Possible Answers:

Enzymes make it so reactions that are nonspontaneous occur spontaneously.

Enzymes supply the water necessary for biochemical reactions.

They help reactions occur at a rate compatible with that necessary for sustenance of life.

Enzymes increase the temperature of the reaction.

Correct answer:

They help reactions occur at a rate compatible with that necessary for sustenance of life.

Explanation:

An enzymes function is to speed up chemical reactions by lowering the activation energy. If our bodies did not have enzymes, the reactions would take place, but too slowly for our cells to adequately function.

Example Question #2 : Enzymes

What is the role of DNA helicase in DNA replication and DNA transcription?

Possible Answers:

DNA helicase disrupts the hydrogen bonding between the two strands of DNA and exposes the insides of the DNA helix, allowing for other enzymes to replicate or transcribe the single-stranded DNA.

None of these

DNA helicase replaces the RNA primers with DNA nucleotides in DNA replication. Helicase has no use in DNA transcription.

DNA helicase binds Okazaki fragments to one another on the lagging strand, and helps create a unified strand of DNA. It has no use in DNA transcription.

DNA helicase relieves the torsional strain and "supercoiling" that DNA undergoes during replication/transcription, and in doing so helps maintain the DNA double helix.

Correct answer:

DNA helicase disrupts the hydrogen bonding between the two strands of DNA and exposes the insides of the DNA helix, allowing for other enzymes to replicate or transcribe the single-stranded DNA.

Explanation:

DNA helicase is an enzyme that is able to slip between the two strands of DNA and disrupt the hydrogen bonds that keep the DNA in the double helix structure. This disruption opens up the DNA helix, and exposes sections of DNA that can then be transcribed or replicated. As helicase moves down the double helix, the DNA reforms into a double helix since the enzyme is no longer blocking the hydrogen bonds.

Example Question #3 : Enzymes

Which of these is a key characteristic of all enzymes?

Possible Answers:

An enzyme reduces the amount of activation energy needed in a system for a certain chemical reaction to occur.

An enzyme is not depleted in a reaction.

An enzyme catalyzes a reaction, allowing it to happen faster than it would without the enzyme.

All of these

An enzyme is a protein product, usually created from 2 or more polypeptide chains. The structure of the enzyme determines the function of the enzyme.

Correct answer:

All of these

Explanation:

These are all definitive traits of an enzyme. Enzymes are proteins which are extremely helpful in speeding up certain reactions without being depleted by the reactions themselves (as such, they are catalysts for these reactions). Enzymes reduce the amount of energy needed for a reaction to occur, generally because they facilitate reactions by recognizing reactants and bringing them into contact with each other. This occurs when the reactants bind to certain parts of the enzyme (active sites), which causes the enzyme to change shape and bring the reactants into contact with each other (and then the reactants can bind to form the product). 

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