All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources
Example Questions
Example Question #31 : Cellular Respiration And Photosynthesis
Cytochrome C is a small protein localized to the inner mitochondrial membrane that has an important role in the last steps of the electron transport chain. What is cytochrome C's primary role in the electron transport chain?
Cytochrome C accepts electrons from complex II and shuttles them to Complex III, which is required for further activation of complex IV
Cytochrome C catalyzes the conversion of 2 ADP to 2 ATP, thus contributing to the energetic gain in the electron transport chain
Cytochrome C removes electrons from complex IV and transfers them to molecular oxygen in the mitochondria
Cytochrome C accepts electrons from complex IV and directly transfers these electrons to molecular oxygen
Cytochrome C accepts electrons from complex III in the mitochondrial membrane and transfers them to complex IV
Cytochrome C accepts electrons from complex III in the mitochondrial membrane and transfers them to complex IV
Cytochrome C is responsible for accepting the electrons generated in the bc1 complex (complex III) and transferring them over to complex IV. In other words, cytochrome C oxidizes complex III and is oxidized by complex IV. This is required to then add those electrons to molecular oxygen, which forms water and contributes to the proton gradient required for ATP production.
Example Question #101 : Biochemistry
What is the final electron acceptor in the electron transport chain?
Cytochrome C
The final electron acceptor in the electron transport chain is oxygen gas. one oxygen gas molecule will accept 4 electrons and combine with 4 protons in order to create 2 water molecules. This reaction is the reason water is a byproduct of aerobic cellular respiration.
Example Question #2 : Help With The Electron Transport Chain
Krebs cycle occurs in the __________ and the electron transport chain occurs in the __________.
cytoplasm . . . mitochondrial matrix
mitochondrial inner membrane . . . mitochondrial matrix
mitochondrial matrix . . . cytoplasm
mitochondrial matrix . . . mitochondrial inner membrane
mitochondrial matrix . . . mitochondrial inner membrane
Glycolysis, the first step in metabolism of carbohydrates, occurs in the cytoplasm. The products of glycolysis (pyruvate and NADH) are transported to the mitochondrial matrix. The products undergo series of reactions called the Krebs cycle. The products of Krebs cycle and NADH from glycolysis enter the inner mitochondrial membrane and go through the electron transport chain (ETC). During ETC, oxidative phosphorylation generates most of the ATP used by the cells.
Example Question #31 : Cellular Respiration And Photosynthesis
Which of the following molecule(s) undergoes reduction during the electron transport chain?
Both of these are reduced
Oxygen
None of these are reduced
NADH
Oxygen
Reduction is the process of gaining electrons. In electron transport chain (ETC), electron carriers such as NADH and donate electrons to the electron carriers in the ETC. These electrons are transported to subsequent molecules. The final acceptor of electrons in ETC is oxygen, which accepts electrons and gets converted into water. Since electrons are being lost from them, NADH and are oxidized in the ETC. On the other hand, oxygen accepts electrons and is reduced in the electron transport chain.
Example Question #32 : Cellular Respiration And Photosynthesis
Oligomycin is an inhibitor of ATP synthase. Which of the following will you observe in the cells of a patient taking oligomycin?
I. There will be a higher concentration of protons in the intermembrane space
II. Proton pump will no longer be functional
III. ATP production will be decreased
I and II
III only
I, II, and III
I and III
I and III
The question states that oligomycin inhibits ATP synthase. Recall that ATP synthase (found on the inner mitochondrial membrane) generates ATP by transporting protons from the intermembrane space (space between inner and outer mitochondrial membrane) into the mitochondria. Inhibiting this will prevent the transport of protons and will, subsequently, lead to a buildup of protons in the intermembrane space.
Proton pumps are also found on the inner mitochondrial membrane. They function to pump out protons from the inside of mitochondria to the intermembrane space, thereby providing the proton gradient for ATP synthase to generate ATP. Halting ATP synthase will cause proton pump to stop pumping protons into the intermembrane space (due to the increase in protons in intermembrane space).
ATP synthase is the major generator of ATP; therefore, halting ATP synthase via oligomycin will decrease the amount of ATP generated.
Example Question #32 : Cellular Respiration And Photosynthesis
During ATP synthesis in mitochondria, protons move directly through __________.
F0 subunit of ATP synthase
Outer mitochondrial membrane
Ubiquinone
Cytoplasmic cellular membrane
F1 subunit of ATP synthase
F0 subunit of ATP synthase
The F0 subunit of ATP synthase is where protons flow through to create ATP. This mechanism involves a rotation of the subunit, producing ATP with each turn. ATP synthase is part of oxidative phosphorylation, the greatest ATP producing segment of cellular respiration.
Example Question #101 : Biochemistry
You are studying three different reduction-oxidation couples in the electron transport chain. Their energies are as follows:
"A" +.02 V
"B" - .31 V
"C" - .41 V
What correctly describes the flow of electrons through these redox couples?
A, B, C
A, C, B
C, B, A
B, C, A
C, A, B
C, B, A
Electrons flow throughout the electron transport chain via redox reactions. They flow from the most negative voltage to the most positive voltage within the chain. Thus the correct flow would be from C (most negative) to B (Less negative) to A (most positive).
Example Question #104 : Biochemistry
Which molecule transfers electrons from photosystem II to photosystem I?
Plastocyanin
Cytochrome c
NADP+
Ferredoxin
Plastocyanin
Photosystems I and II are each capable of conducting electrons, with photosystem II handing off electrons to photosystem I. This is accomplished by the electron carrier molecule plastocyanin.
Example Question #105 : Biochemistry
Which product is made in photosystem I?
ATP
NADPH
NADH
Glucose
NADPH
Photosystems I and II are responsible for the light-dependent reactions of photosynthesis. These two photosystems work in tandem to create ATP and NADPH products. ATP is created in photosystem II, while NADPH is created in photosystem I.
Example Question #3 : Help With Light Reactions
What fuels ATP synthase to make ATP from ADP + Pi in the light reaction of photosynthesis?
Light excites photosystem II to split water into hydrogen and oxygen. Oxygen accumulates in the thylakoid space. Oxygen then moves down its concentration gradient from the thylakoid space to the stroma by passing through ATP synthase, fueling the synthesis of ATP
NADPH is formed from NADP+ reductase in the thylakoid membrane. NADPH can then donate hydrogen to ATP synthase to fuel the synthesis of ATP
Light excites photosystem II to split water into hydrogen and oxygen. Hydrogen ions accumulate in the thylakoid space. Hydrogen moves down its concentration gradient from the thylakoid space to the stroma by passing through ATP synthase, fueling the synthesis of ATP
The mechanism by which ATP synthase is fueled is not entirely known
Light excites photosystem I to generate electrons that pass through the thylakoid membrane to excite ATP synthase to generate ATP
Light excites photosystem II to split water into hydrogen and oxygen. Hydrogen ions accumulate in the thylakoid space. Hydrogen moves down its concentration gradient from the thylakoid space to the stroma by passing through ATP synthase, fueling the synthesis of ATP
Excitation of photosystem II splits water in the thylakoid space into hydrogen and oxygen. The hydrogen then passes through ATP synthase to move down its concentration gradient and into the stroma. Excitation of photosystem I passes electrons to NADP+ reductase to convert NADP+ to NADPH. Regeneration of NADPH is necessary for the Calvin cycle.
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