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Example Questions
Example Question #3 : Properties Of Nucleic Acids
In DNA, cytosine always pairs with __________, and adenine always pairs with __________.
thymine . . . thymine
guanine . . . thymine
thymine . . . guanine
uracil . . . thymine
guanine . . . thymine
Austrian chemist Erwin Chargaff discovered that the amount of cytosine was equal to the amount of guanine in the cell, and the amount of adenine was equal to the amount of thymine in the cell. This discovery was important in determining that cytosine always pairs with guanine and that adenine always pairs with thymine.
Example Question #4 : Properties Of Nucleic Acids
__________ is found in RNA but not in DNA.
Uracil
Adenine
Cytosine
Thymine
Uracil
In RNA, uracil pairs with adenine and cytosine pairs with guanine, whereas in DNA, thymine pairs with adenine and cytosine pairs with guanine. Uracil is unique to RNA and thymine is unique to DNA.
Example Question #2 : Properties Of Nucleic Acids
Which set of base pairs would have the higher boiling point?
GCAATTTTT
CATCAT
AATTGCAT
GGCCATCG
GGCCATCG
G-C is held together by three hydrogen bonds, while A-T is held together by two hydrogen bonds. Having more hydrogen bonds means that boiling points would be higher, so the set of base pairs with the most C-G bonds will have the highest boiling point.
Example Question #1 : Properties Of Nucleic Acids
If a double-stranded DNA fragment was analyzed and found to have 270 adenine bases and 430 guanine bases, how many total bases would necessarily be present in the DNA fragment?
1400
700
2800
1080
1720
1400
Since adenine pairs with thymine in DNA, this would mean there are also 270 thymine bases. Similarly, cytosine and guanine pair up as well. This would mean there are also 430 cytosine bases. Adding all those together would give you 1400 bases total.
Example Question #4 : Properties Of Nucleic Acids
Of which of the following is a nucleoside comprised?
Base and phosphate group
Sugar and phosphate group
Base only
Base and sugar
Sugar, base, and phosphate group
Base and sugar
A nucleotide comprises of a purine/pyrimidine base, sugar, and a phosphate group. This is easy remember by focusing on the "t" in the terms "nucleotide" and "phosphate."
A nucleoside comprises only a purine/pyrimidine base and sugar. There is no "t" in "nucleoside" and hence, no phosphate.
Example Question #5 : Properties Of Nucleic Acids
Which nucleic acid brings amino acids to the site of protein synthesis, adding to the growing amino acid chain?
mRNA
tRNA
xDNA
mtDNA
rRNA
tRNA
All of these are actual nucleic acids, except xDNA, which does not exist. Therefore, we can eliminate this answer right away. mtDNA, or mitochondrial DNA, is a small portion of a person's DNA that is housed within the mitochondria. It is significant in that it is inherited solely from the mother and is thought to have evolved separately from "normal" DNA. However, it isn't involved with amino acids at all, so it too can be eliminated as a choice.
RNA (ribonucleic acid) is heavily involved in protein synthesis (the creation of new proteins, which are just long chains of amino acids). The first part of the process is transcription, in which the double-stranded DNA (deoxyribonucleic acid) is "unzipped" by the enzyme helicase. Another enzyme, RNA polymerase, "reads" this single strand and produces a chain of nucleotides exactly opposite to the DNA's nucleotides, and this chain, in its mature form, is called mRNA (messenger RNA). Living up to its name, mRNA travels outside the nucleus, where DNA is housed, and out into the rest of the cell, to the ribosome.
At this point, translation begins. Every trio of nucleotides on the mRNA, called a codon, represents a specific amino acid. Another type of RNA, tRNA (transfer RNA) can "translate" the codon into the appropriate amino acid, since it carries a three-nucleotide-long anticodon on one segment, which can form a bond with the corresponding codon on the mRNA, and an amino acid on the other segment. In this way tRNA brings amino acids to the growing protein chain. Another involved nucleic acid is rRNA (ribosomal RNA) which makes up a large part of the ribosome itself and is responsible for helping to properly attach each amino acid that the tRNA brings to the amino acid sequence being built.
Based on the question, which asks about the molecule that retrieves amino acids so that they can be added to the protein being built, the correct answer is tRNA.
Example Question #11 : Properties Of Nucleic Acids
Which nucleic acid carries information coding for an amino acid sequence out from the nucleus towards a ribosome in the cytoplasm?
rRNA
xDNA
mRNA
mDNA
tRNA
mRNA
All of these are actual nucleic acids, except xDNA, which does not exist. Therefore, we can eliminate this answer right away. mtDNA, or mitochondrial DNA, is a small portion of a person's DNA that is housed within the mitochondria. It is significant in that it is inherited solely from the mother and is thought to have evolved separately from "normal" DNA; however, it isn't involved with amino acids at all, so it too can be eliminated as a choice.
RNA (ribonucleic acid) is heavily involved in protein synthesis (the creation of new proteins, which are just long chains of amino acids). The first part of the process is transcription, in which the double-stranded DNA (deoxyribonucleic acid) is "unzipped" by the enzyme helicase. Another enzyme, RNA polymerase, "reads" this single strand and produces a chain of nucleotides exactly opposite to the DNA's nucleotides, and this chain, in its mature form, is called mRNA (messenger RNA). Living up to its name, mRNA travels outside the nucleus, where DNA is housed, and out into the rest of the cell, to the ribosome.
At this point, translation begins. Every trio of nucleotides on the mRNA, called a codon, represents a specific amino acid. Another type of RNA, tRNA (transfer RNA) can "translate" the codon into the appropriate amino acid, since it carries a three-nucleotide-long anticodon on one segment, which can form a bond with the corresponding codon on the mRNA, and an amino acid on the other segment. In this way tRNA brings amino acids to the growing protein chain. Another involved nucleic acid is rRNA (ribosomal RNA) which makes up a large part of the ribosome itself and is responsible for helping to properly attach each amino acid that the tRNA brings to the amino acid sequence being built.
Based on the question, which asks you to identify the nucleic acid that helps carry the protein "message" from the nucleus to the ribosome, the correct answer is mRNA.
Example Question #11 : Properties Of Nucleic Acids
Which of the following codons serves as the initiation site when a mRNA transcription begins to be translated?
AUG
UAG
UAA
CUG
UGA
AUG
In DNA transcription and translation, the start codon is the first codon, among those transcribed into the mRNA sequence, that is translated during protein synthesis. In humans, this is always AUG, which codes for the amino acid methionine.
Of the other answer choices, three function as a stop codon: UAA, UGA, and UAG. The other choice, CUG, codes for the amino acid leucine.
A mnemonic for remembering that AUG is the start codon is to make the connection that the school year typically starts in August.
Example Question #12 : Properties Of Nucleic Acids
After sequencing the genome of a newly discovered virus (virus A), you find that it is composed of 18% Adenine bases, consists of one double stranded loop, and contains deoxyribose sugar.
What percentage of the genome is composed of Guanine bases?
64%
36%
18%
32%
32%
According to Chargoff's rules, we know that the ratios of Adenine:Thymine and Guanine: Cytosine will be approximately 1:1. Once you know that the percentage of Adenine is 18%, you can multiply by two to find the total percentage of Thymine and Adenine (36%), then, you can subtract that number from 100% to find the percentage of Cytosine and Guanine - 64% (because the total percentage has to equal 100%), finally you can divide it in half to get the percentage of Guanine bases (32%).
Example Question #13 : Properties Of Nucleic Acids
After sequencing the genome of a newly discovered virus (virus A), you find that it is composed of 18% Adenine bases, consists of one double stranded loop, and contains deoxyribose sugar.
What percentage of the genome is composed of Uracil bases?
36%
0%
64%
18%
0%
Because of the presence of Deoxyribose sugar, you know that the genome is composed of DNA. Only RNA contains Uracil bases, so the answer is 0%.