Biochemistry : Biochemistry

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #1 : Glycosidic Linkages

Glycogen is not a single chain of glucose units, but many chains branching off of one another. Why is the branching of glycogen important?

Possible Answers:

All of these are reasons why glycogen branching is important

Branching makes glycogen more compact

Branching increases the rate of glycogen synthesis

Branching increases the rate of glycogen degradation

Branching increases glycogen solubility

Correct answer:

All of these are reasons why glycogen branching is important

Explanation:

Because glycogen is so heavily branched, it is able to pack more glucose units together in a small space, thus it is more compact and has a greater solubility. Moreover, the branching allows for glycogen enzymes to act more efficiently on the chains of glucose, and so both degradation and synthesis have increased rates.

Example Question #1 : Glycosidic Linkages

You discover that your patient is lactose intolerant, having a mutation that does not allow them to produce an enzyme that cleaves the disaccharide lactose. If they had the lactase enzyme, which glycosidic bond would it cleave?

Possible Answers:

Glucose-alpha 1,2-fructose

Galactose-alpha-1,4-glucose

Glucose-beta 1,4-glucose 

Glucose-alpha-1,4-glucose

Galactose-beta-1,4-glucose 

Correct answer:

Galactose-beta-1,4-glucose 

Explanation:

lactose is made up of galactose and glucose and is bound via a beta 1,4 glycosidic bond. 

the enzyme lactase cleaves this bond to break down the sugar lactose. Maltose is glucose- alpha 1,4- glucose, and sucrose is glucose- alpha, 1,2- fructose. 

Example Question #2 : Carbohydrate Structures And Functions

What is an aldotriose?

Possible Answers:

A monosaccharide that contains three aldehydes and one carbon

A disaccharide that contains three aldehydes and one carbon

A monosaccharide that contains both an aldehyde and three carbons

A disaccharide that contains an aldehyde and three carbons

Correct answer:

A monosaccharide that contains both an aldehyde and three carbons

Explanation:

Aldotrioses are monosaccharides that contain both an aldehyde (an aldose) and three carbons (a triose). Knowing the definition of the word, and the breakdown of parts of the word, can help you recognize the molecule. The simplest aldotriose is glyceraldehyde.

A related concept involves ketotrioses, which are monosaccharides that contain both a ketone (a ketose) and three carbonds (a triose). Dihydroxyacetone is an example of a ketotriose.

Example Question #1 : Epimers, Chirality, And The Anomeric Carbon

Mirror-image stereoisomers are called __________.

Possible Answers:

enantiomers

epimers

anomers

diastereomers

Correct answer:

enantiomers

Explanation:

Enantiomers are chiral molecules that are non-superimposable mirror images of each other. Diastereomers result when two or more stereoisomers of a compound have different configurations at one or more (but not all) of the equivalent stereocenters and are not mirror images of each other. An epimer is one of two stereoisomers that differ in configuration at only one stereocenter. An anomer is a type of epimer; it is one of two stereoisomers of a cyclic sugar that differs only in its configuration at the hemiacetal or acetal carbon (the anomeric carbon).

Example Question #2 : Carbohydrate Structures And Functions

Why is it that reducing sugars can be metabolized in humans, but non-reducing sugars cannot?

Possible Answers:

Because reducing sugars can bind to the proteins needed for metabolism, whereas non-reducing sugars cannot

Because only reducing sugars can traverse the cell membrane in order to enter cells where they can be metabolized, whereas non-reducing sugars cannot

Because reducing sugars can open their cyclic structure into the straight chain form, whereas non-reducing sugars cannot

Because humans lack the enzyme that degrades beta glycosidic linkages

Correct answer:

Because reducing sugars can open their cyclic structure into the straight chain form, whereas non-reducing sugars cannot

Explanation:

When it comes to metabolizing sugars, only reducing sugars are able to undergo breakdown. This is because reducing sugars are able to be converted from their closed chain form into their open chain form. It is only in the open chain form that sugars such as glucose can be metabolized.

Only reducing sugars can be converted into their open chain form. The reason for this is that the anomeric carbon for these sugars is not occupied. In their ring form, such sugars exist as hemiacetals that can readily and reversibly undergo chain opening. Additionally, some hemiketals can be converted into their open chain form, but they need to be able to tautomerize into their aldose form first.

Non-reducing sugars have their anomeric carbon tied up in a bond, and thus are locked in an acetal or ketal form. Consequently, they cannot convert into their open chain form, meaning that they cannot be metabolized.

Example Question #1 : Carbohydrate Structures And Functions

Which of the following statements about carbohydrates is true?

Possible Answers:

Amylose makes up the major component of starch by mass

Glucose is a sugar with six hydroxyl groups and an aldehyde

Sucrose is a reducing sugar

None of the other statements is true

Polysaccharides have glycosidic bonds

Correct answer:

Polysaccharides have glycosidic bonds

Explanation:

Glucose has five (not six) hydroxyl groups. Reducing sugars either have an aldehyde group or can form one through isomerism; sucrose doesn’t fit either description. Although there are more amylose molecules than amylopectin in starch, amylose is a minor component by mass; amylopectin makes up 70-80% of starch by mass. Polysaccharides are indeed joined in the union of two oses, which form glycosidic bonds.

Example Question #1 : Carbohydrate Structures And Functions

What functional groups are present on carbohydrates?

Possible Answers:

Hydroxyl

Amide

Phosphate

Carboxyl

Alcohol

Correct answer:

Carboxyl

Explanation:

Carbohydrate chains contain aldehyde or ketone functional groups, which are types of carboxyl groups. Remember the general formula for a carbohydrate is:  since they are hydrates (water) of carbon.

Example Question #191 : Macromolecule Structures And Functions

Which of the following is not an example of a compound that contains carbohydrate in its structure?

Possible Answers:

Glycogen

Collagen

Peptidoglycan

Cellulose

Chitin

Correct answer:

Collagen

Explanation:

To identify the answer choice that is NOT a polysaccharide, let's go delve into each answer choice a bit.

Cellulose is a polysaccharide and also a very important component of the cell wall of plants. It consists of many glucose sugars bound together via beta (1-4) linkages.

Peptidoglycan is also a polysaccharide and a very important component of the cell wall of bacteria. Its structure consists of alternating N-acetylglucosamine and N-acetylmuramic acid connected via beta (1-4) linkages. Both of these are modified sugar molecules.

Chitin is a polysaccharide that plays an important role in the structure of the exoskeleton of many fungi, arthropods, and insects. Its structure consists of a long chain of N-acetylglucosamine, which is a derivative of the sugar glucose.

Collagen is the only answer choice that does not represent a carbohydrate. Rather, collagen is a protein that plays a very important role in the extracellular matrix of various connective tissues found in animals.

Glycogen is a branched form of glucose that is the storage form in animals. It contains beta (1-4) and beta (1-6) linkages.

Example Question #1 : Structural Carbohydrates

Both cellulose and starch are polymers of glucose; why can't and enzyme that breaks down starch also break down cellulose?

Possible Answers:

Cellulose is composed of D-glucose molecules while starch is composed of L-glucose molecules

Glucose monomers in starch are double bonded together while they are connected by single bonds in cellulose

Cellulose forms a right handed helix, while starch forms a left handed helix

Cellulose is composed of cyclic glucose molecules while starch is composed of linear glucose molecules

Cellulose and starch are made up of different stereoisomers of glucose

Correct answer:

Cellulose and starch are made up of different stereoisomers of glucose

Explanation:

Cyclized monosaccharides can exist in two different stereoisomers that depend on the orientation of the hydroxyl group on the anomeric carbon. If this  group is on the opposite side of the ring as the  group, the sugar as the alpha anomer. The opposite orientation is referred to at the beta anomer. Cellulose is a polymer of beta glucose while starch is a polymer of alpha glucose, and the bonds between these glucose monomers differ depending on their anomer. This makes it so an enzyme can recognize one or the other but not both. Note that D-sugars are biologically relevant. 

Example Question #192 : Macromolecule Structures And Functions

Which of the following is not a glycosaminoglycan?

Possible Answers:

Erythropoietin 

Hyaluronate

Keratan sulfate

Heparin

Dermatan sulfate

Correct answer:

Erythropoietin 

Explanation:

A glycosaminoglycan (GAG) is a long, unbranched polysaccharide that consists of many repeating disaccharide units. Dermatan sulfate, keratan sulfate, hyaluronate, and heparin are all examples of glycosaminoglycans. Erythropoietin, however, is a glycoprotein hormone that stimulates production of red blood cells.

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