AP Biology : Microorganisms and Viruses

Study concepts, example questions & explanations for AP Biology

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Example Questions

Example Question #11 : Understanding Bacteria

Which of the following eukaryotic cellular processes does not occur in bacterial cells?

Possible Answers:

Replication

Mitosis

Translation

Transcription

Correct answer:

Mitosis

Explanation:

Similar to eukaryotes, bacterial cells are in accordance with the central dogma of molecular biology. The flow of genetic information involves replication, transcription, and translation. Bacterial cells have to replicate their genetic material (replication), undergo transcription to convert DNA to mRNA, and go through translation to produce proteins from the mRNA.

Bacterial cells do not undergo mitosis or meiosis. Cell division can occur through other modes, such as binary fission. Since bacteria lack nuclei and other complex structures, their processes for cellular division are much more abbreviated and do not follow the same steps as eukaryotic mitosis.

Example Question #11 : Microorganisms And Viruses

Which of the following is true regarding gram-positive and gram-negative bacteria?

Possible Answers:

Gram-positive bacteria have half as many cell membranes as gram-negative bacteria

Gram-negative bacteria have a thicker cell wall

Gram-negative bacteria appear purple when gram stained

Only gram-positive bacteria contain peptidoglycan cell walls

Correct answer:

Gram-positive bacteria have half as many cell membranes as gram-negative bacteria

Explanation:

First, remember that all bacterial cell walls contain peptidoglycan. The difference between gram-positive and gram-negative bacterial cell walls rises from the composition of peptidoglycan. Gram-negative bacteria appear pink when observed through a microscope, not purple. Also, gram-negative bacteria have thinner cell walls than their counterparts.

Gram-positive bacteria have half as many cell membranes as gram-negative bacteria. This is true because gram-negative bacteria contain an extra cell membrane outside their cell wall. Gram-negative bacteria have two cell membranes, whereas gram-positive bacteria only have one.

Example Question #11 : Understanding Bacteria

What happens to a bacterial cell if you place it in a hypotonic solution?

Possible Answers:

It swells because the cytosol contains more solute

It shrivels because the cytosol contains less solute

It shrivels because the cytosol contains more solute

It swells because the cytosol contains less solute

Correct answer:

It swells because the cytosol contains more solute

Explanation:

If you place a cell in a hypotonic solution it means that the solution contains less solute (and more water) than the cell’s cytosol. Recall that osmosis causes water to go from a region of high water content (few solutes) to a region of low water content (more solutes).

In this case, the water will go from the solution to the inside of the cell, causing the cell to swell.

Example Question #11 : Microorganisms And Viruses

Staphylococcus aureus is a bacterium that causes skin infections and respiratory disease. What shape is this bacterium?

Possible Answers:

Helical

Rod

Irregular

Round

Correct answer:

Round

Explanation:

The name staphylococcus aureus contains the term "coccus," which indicates that the bacteria is round-shaped.

Rod shaped bacteria are classified as bacilli and helical bacteria are classified as spirilla.

Example Question #13 : Understanding Bacteria

One mode of bacterial reproduction involves the formation of a bridge between two adjacent bacterial cells. This bridge is used to transport genetic material from one cell to the other. This mode of reproduction is called __________.

Possible Answers:

conjugation

transformation

translation

transduction

Correct answer:

conjugation

Explanation:

Formation of a bridge between two cells occurs during conjugation. The bridge is called a sex pilus, which is used to carry genetic material from one bacterial cell to the other.

Transduction is another mode of reproduction, but involves transmission of genetic material through a vector. Vectors are generally host cells or virions that carry parts of the bacterial genome known as plasmids.

The third type of bacterial reproduction is called transformation. In transformation a bacterial cell engulfs and incorporates genetic material from its surroundings.

Translation is not a type of bacterial reproduction; it is the process by which proteins are made from mRNA.

Example Question #12 : Understanding Bacteria

What compound is present in both gram-positive and gram-negative bacteria?

Possible Answers:

Outer membrane

Teichoic acids

Lipopolysaccharides

Peptidoglycan

Correct answer:

Peptidoglycan

Explanation:

Only gram-negative bacteria have an outer membrane. This outer membrane contains lipopolysaccharides. Gram-positive bacteria have only one membrane.

Teichoic acids are present only in gram-positive bacteria. They contribute to the thickness of the peptidoglycan layer.

Both types of bacteria have peptidoglycan. Gram-positive bacteria have a thick layer, due to the presence of teichoic acids. This allows them to retain the gram stain and appear purple. Gram-negative bacteria have a thin peptidoglycan layer, causing them to appear pink under gram staining.

Example Question #12 : Microorganisms And Viruses

A researcher performs gram-staining on two different bacterial cells. Using a microscope, he observes that cell A is pink whereas cell B is purple. Based on this information, what can he conclude about the two bacterial cells?

Possible Answers:

Cell B is gram-negative and has a thicker cell wall

Cell B is gram-positive and has a thicker cell wall

Cell A is gram-negative and has a thicker cell wall

Cell A is gram-positive and has a thicker cell wall

Cell A is gram-negative, but no conclusion can be made about its cell wall

Correct answer:

Cell B is gram-positive and has a thicker cell wall

Explanation:

Recall that pink stained cells are gram-negative, whereas purple stained cells are gram-positive. Based on color, we can conclude that cell A is gram-negative (pink) and cell B is gram-positive (purple). One of the characteristics of gram-positive cells is that they have a thick cell wall. The thick peptidoglycan cell wall stops the gram stain from leaking out, resulting in a darker color.

Since cell B is purple, we can conclude that it is gram-positive and has a thick cell wall.

Example Question #17 : Microorganisms And Viruses

An opportunistic pathogen that is an acidophile would cause the biggest concern if entering the body through which natural barrier?

Possible Answers:

The genito-urinary system

Mucous membranes

The digestive system

The organism would be equally dangerous regardless of the portal of entry

The respiratory system

Correct answer:

The digestive system

Explanation:

The genito-urinary, respiratory, and digestive systems, as well as skin and mucous membranes, are natural physical barriers against infection.

Opportunistic microorganisms are normal fauna that are usually harmless; however, given the opportunity of a breach of any of our natural barriers, these organisms can invade vulnerable systems and cause infection, thus becoming pathogenic.

A pathogen that is an acidophile prefers highly acidic environments and therefore could survive the stomach's 2.0 pH - a lethal barrier to most organisms. The effectiveness of other natural barriers, however, would not be different because the organism is an acidophile.

Example Question #18 : Microorganisms And Viruses

Which is not a difference between prokaryotes and eukaryotes?

Possible Answers:

Prokaryotes have a circular chromosome

Eukaryotes have multiple replication origins

Eukaryotes have membrane-bound organelles

Prokaryotes do not have telomeres

None of these

Correct answer:

None of these

Explanation:

All of these answers are true differences between prokaryotes and eukaryotes. Prokaryotes' DNA is double-stranded, but circular instead of linear, as in eukaryotes. This facilitates the expedient replication of multiple genes.  Eukaryotes have membrane-bound organelles, but prokaryotes do not. The more complex a cell is, the greater number of reactions and processes that need to be separated. Eukaryotes also have multiple replication origins most likely due to the length and distribution of their genes. Eukaryotes also do not have operons (present in prokaryotes) that are analogous to "gene packets," which are transcribed simultaneously. Since prokaryotic DNA is circular they have no "ends". Thus there are no need for telomeres which cover the ends of eukaryotic chromosomes. This loss of important genetic information at the end of transcription due to the antiparallel nature of DNA.

Example Question #13 : Understanding Bacteria

Which description does not apply to bacteria?

Possible Answers:

Cellular organelles that are membrane-bound

DNA that is double-stranded

Ribosomes that synthesize polypeptides

A plasma membrane made up of lipids and proteins

Ability to undergo glycolysis

Correct answer:

Cellular organelles that are membrane-bound

Explanation:

Bacteria are a type of prokaryote, which do not have any membrane-bound organelles, but do have circular, doubled-stranded DNA, ribosomes, and an outer membrane made of proteins and lipids. Since glycolysis does not require any organelles, it occurs in they cytoplasm of both prokaryotes and eukaryotes.

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