Passage 1 adapted from "On the Death of Marie Antoinette" by Edmund Burke (1793)

It is now sixteen or seventeen years since I saw the Queen of France, then the Dauphiness, at Versailles; and surely never lighted on this orb, which she hardly seemed to touch, a more delightful vision. I saw her just above the horizon, decorating and cheering the elevated sphere she had just begun to move in, glittering like the morning star full of life and splendor and joy.

Oh, what a revolution! And what a heart must I have, to contemplate without emotion that elevation and that fall! Little did I dream, when she added titles of veneration to those of enthusiastic, distant, respectful love, that she should ever be obliged to carry the sharp antidote against disgrace concealed in that bosom; little did I dream that I should have lived to see such disasters fallen upon her, in a nation of gallant men and of cavaliers! I thought ten thousand swords must have leaped from their scabbards, to avenge even a look that threatened her with insult.

But the age of chivalry is gone; that of sophistry, economists, and calculators has succeeded, and the glory of Europe is extinguished forever. Never, never more, shall we behold that generous loyalty to rank and sex, that proud submission, that dignified obedience, that subordination of the heart, which kept alive, even in servitude itself, the spirit of an exalted freedom! The unsought grace of life, the cheap defense of nations, the nurse of manly sentiment and heroic enterprise is gone. It is gone, that sensibility of principle, that chastity of honor, which felt a stain like a wound, which inspired courage whilst it mitigated ferocity, which ennobled whatever it touched, and under which vice itself lost half its evil, by losing all its grossness.

Passage 2 adapted from Thomas Paine’s The Rights of Man (1791)

The case, therefore, divides itself into two parts; the right which they possessed by delegation, and the right which they set up by assumption. The first is admitted; but with respect to the second, I reply-

There never did, there never will, and there never can, exist a Parliament, or any description of men, or any generation of men, in any country, possessed of the right or the power of binding and controlling posterity to the "end of time," or of commanding for ever how the world shall be governed, or who shall govern it; and therefore all such clauses, acts or declarations by which the makers of them attempt to do what they have neither the right nor the power to do, nor the power to execute, are in themselves null and void. Every age and generation must be as free to act for itself in all cases as the age and generations which preceded it. The vanity and presumption of governing beyond the grave is the most ridiculous and insolent of all tyrannies. Man has no property in man; neither has any generation a property in the generations which are to follow. The Parliament or the people of 1688, or of any other period, had no more right to dispose of the people of the present day, or to bind or to control them in any shape whatever, than the parliament or the people of the present day have to dispose of, bind or control those who are to live a hundred or a thousand years hence. Every generation is, and must be, competent to all the purposes which its occasions require. It is the living, and not the dead, that are to be accommodated. When man ceases to be, his power and his wants cease with him; and having no longer any participation in the concerns of this world, he has no longer any authority in directing who shall be its governors, or how its government shall be organised, or how administered.

I am not contending for nor against any form of government, nor for nor against any party, here or elsewhere. That which a whole nation chooses to do it has a right to do. Mr. Burke says, No. Where, then, does the right exist? I am contending for the rights of the living, and against their being willed away and controlled and contracted for by the manuscript assumed authority of the dead, and Mr. Burke is contending for the authority of the dead over the rights and freedom of the living. There was a time when kings disposed of their crowns by will upon their death-beds, and consigned the people, like beasts of the field, to whatever successor they appointed. This is now so exploded as scarcely to be remembered, and so monstrous as hardly to be believed.

What is the relationship between these two passages?

Passage 1 adapted from "On the Death of Marie Antoinette" by Edmund Burke (1793)

It is now sixteen or seventeen years since I saw the Queen of France, then the Dauphiness, at Versailles; and surely never lighted on this orb, which she hardly seemed to touch, a more delightful vision. I saw her just above the horizon, decorating and cheering the elevated sphere she had just begun to move in, glittering like the morning star full of life and splendor and joy.

Oh, what a revolution! And what a heart must I have, to contemplate without emotion that elevation and that fall! Little did I dream, when she added titles of veneration to those of enthusiastic, distant, respectful love, that she should ever be obliged to carry the sharp antidote against disgrace concealed in that bosom; little did I dream that I should have lived to see such disasters fallen upon her, in a nation of gallant men and of cavaliers! I thought ten thousand swords must have leaped from their scabbards, to avenge even a look that threatened her with insult.

But the age of chivalry is gone; that of sophistry, economists, and calculators has succeeded, and the glory of Europe is extinguished forever. Never, never more, shall we behold that generous loyalty to rank and sex, that proud submission, that dignified obedience, that subordination of the heart, which kept alive, even in servitude itself, the spirit of an exalted freedom! The unsought grace of life, the cheap defense of nations, the nurse of manly sentiment and heroic enterprise is gone. It is gone, that sensibility of principle, that chastity of honor, which felt a stain like a wound, which inspired courage whilst it mitigated ferocity, which ennobled whatever it touched, and under which vice itself lost half its evil, by losing all its grossness.

Passage 2 adapted from Thomas Paine’s The Rights of Man (1791)

The case, therefore, divides itself into two parts; the right which they possessed by delegation, and the right which they set up by assumption. The first is admitted; but with respect to the second, I reply-

There never did, there never will, and there never can, exist a Parliament, or any description of men, or any generation of men, in any country, possessed of the right or the power of binding and controlling posterity to the "end of time," or of commanding for ever how the world shall be governed, or who shall govern it; and therefore all such clauses, acts or declarations by which the makers of them attempt to do what they have neither the right nor the power to do, nor the power to execute, are in themselves null and void. Every age and generation must be as free to act for itself in all cases as the age and generations which preceded it. The vanity and presumption of governing beyond the grave is the most ridiculous and insolent of all tyrannies. Man has no property in man; neither has any generation a property in the generations which are to follow. The Parliament or the people of 1688, or of any other period, had no more right to dispose of the people of the present day, or to bind or to control them in any shape whatever, than the parliament or the people of the present day have to dispose of, bind or control those who are to live a hundred or a thousand years hence. Every generation is, and must be, competent to all the purposes which its occasions require. It is the living, and not the dead, that are to be accommodated. When man ceases to be, his power and his wants cease with him; and having no longer any participation in the concerns of this world, he has no longer any authority in directing who shall be its governors, or how its government shall be organised, or how administered.

I am not contending for nor against any form of government, nor for nor against any party, here or elsewhere. That which a whole nation chooses to do it has a right to do. Mr. Burke says, No. Where, then, does the right exist? I am contending for the rights of the living, and against their being willed away and controlled and contracted for by the manuscript assumed authority of the dead, and Mr. Burke is contending for the authority of the dead over the rights and freedom of the living. There was a time when kings disposed of their crowns by will upon their death-beds, and consigned the people, like beasts of the field, to whatever successor they appointed. This is now so exploded as scarcely to be remembered, and so monstrous as hardly to be believed.

On which statement would the author of these two passages agree?

Passage 1 adapted from "On the Death of Marie Antoinette" by Edmund Burke (1793)

It is now sixteen or seventeen years since I saw the Queen of France, then the Dauphiness, at Versailles; and surely never lighted on this orb, which she hardly seemed to touch, a more delightful vision. I saw her just above the horizon, decorating and cheering the elevated sphere she had just begun to move in, glittering like the morning star full of life and splendor and joy.

Oh, what a revolution! And what a heart must I have, to contemplate without emotion that elevation and that fall! Little did I dream, when she added titles of veneration to those of enthusiastic, distant, respectful love, that she should ever be obliged to carry the sharp antidote against disgrace concealed in that bosom; little did I dream that I should have lived to see such disasters fallen upon her, in a nation of gallant men and of cavaliers! I thought ten thousand swords must have leaped from their scabbards, to avenge even a look that threatened her with insult.

But the age of chivalry is gone; that of sophistry, economists, and calculators has succeeded, and the glory of Europe is extinguished forever. Never, never more, shall we behold that generous loyalty to rank and sex, that proud submission, that dignified obedience, that subordination of the heart, which kept alive, even in servitude itself, the spirit of an exalted freedom! The unsought grace of life, the cheap defense of nations, the nurse of manly sentiment and heroic enterprise is gone. It is gone, that sensibility of principle, that chastity of honor, which felt a stain like a wound, which inspired courage whilst it mitigated ferocity, which ennobled whatever it touched, and under which vice itself lost half its evil, by losing all its grossness.

Passage 2 adapted from Thomas Paine’s The Rights of Man (1791)

The case, therefore, divides itself into two parts; the right which they possessed by delegation, and the right which they set up by assumption. The first is admitted; but with respect to the second, I reply-

There never did, there never will, and there never can, exist a Parliament, or any description of men, or any generation of men, in any country, possessed of the right or the power of binding and controlling posterity to the "end of time," or of commanding for ever how the world shall be governed, or who shall govern it; and therefore all such clauses, acts or declarations by which the makers of them attempt to do what they have neither the right nor the power to do, nor the power to execute, are in themselves null and void. Every age and generation must be as free to act for itself in all cases as the age and generations which preceded it. The vanity and presumption of governing beyond the grave is the most ridiculous and insolent of all tyrannies. Man has no property in man; neither has any generation a property in the generations which are to follow. The Parliament or the people of 1688, or of any other period, had no more right to dispose of the people of the present day, or to bind or to control them in any shape whatever, than the parliament or the people of the present day have to dispose of, bind or control those who are to live a hundred or a thousand years hence. Every generation is, and must be, competent to all the purposes which its occasions require. It is the living, and not the dead, that are to be accommodated. When man ceases to be, his power and his wants cease with him; and having no longer any participation in the concerns of this world, he has no longer any authority in directing who shall be its governors, or how its government shall be organised, or how administered.

I am not contending for nor against any form of government, nor for nor against any party, here or elsewhere. That which a whole nation chooses to do it has a right to do. Mr. Burke says, No. Where, then, does the right exist? I am contending for the rights of the living, and against their being willed away and controlled and contracted for by the manuscript assumed authority of the dead, and Mr. Burke is contending for the authority of the dead over the rights and freedom of the living. There was a time when kings disposed of their crowns by will upon their death-beds, and consigned the people, like beasts of the field, to whatever successor they appointed. This is now so exploded as scarcely to be remembered, and so monstrous as hardly to be believed.

The author of Passage 1 would most likely think that the assertions about the limits of ruling power made in Passage 2 were ___________________.

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

Which of the following conclusions can be drawn from the passage?

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

Which choice provides the best evidence for the answer to the previous question?

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

From the second paragraph, it can be inferred that __________.

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

Which choice provides the best evidence for the answer to the previous question?

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

In the first paragraph, the word crepuscular most closely means __________.

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

The purpose of the second paragraph is most likely to __________.

Individuals of the roughly 2,000 species in the family Lampyridae include those insects capable of producing bioluminescent light through a specific metabolic process. Though commonly referred to as fireflies or lightning bugs, these idiosyncratic creatures are more accurately categorized as winged beetles. Like their amphibian predators, most fireflies are crepuscular and are thus largely reliant on their bioluminescence to attract mates, find food, and warn predators of their potential poisonousness. Fireflies are known not to be desirable prey animals for most predators due to the presence of potentially harmful substances in their blood and bitter taste. During their larval stage, bioluminescence serves as the primary defense mechanism to fend off those predators. The diet of most fireflies includes a mixture of nectar, pollen, fireflies, and other insects. It has been shown that different species of fireflies exhibit unique bioluminescence patterns when attracting mates. For example, males of the species P. pyralis (the state insect of Tennessee) use flashing patterns during courtship to attract potential mates. If a female elects to mate with the male, she will respond by reciprocating with a flash of her own. However, the males must beware, as females of other species such as P. pensylvanica can mimic these patterns to deceive, attract, and eat the males.

The biochemical reaction by which fireflies produce light occurs inside a specialized organ in their lower abdomen. This light-emitting organ utilizes the molecule luciferin, which is responsible for the production of visible light. In the presence of oxygen, magnesium ions, and the energy-rich molecule adenosine triphosphate (ATP), the enzyme luciferase converts luciferin into oxyluciferin, which emits light due to being in an electronically excited state. Upon emitting light, oxyluciferin is recycled and reconverted to luciferin so the process may continue. As with any biochemical process, the rate and capacity for bioluminescence in fireflies is dictated by the concentration of inputs as well as the rate at which byproducts are recycled. Scientists still do not fully understand how fireflies are able to produce bioluminescence with upwards of 80-90% energy efficiency. In comparison, the average incandescent light bulbs and LED lights emit only about 10% and 20% of their total electrical energy input as light, respectively. Since the first law of thermodynamics states that the total energy of the universe is constant and energy can neither be created nor destroyed, heat is the major byproduct in the reactions mentioned above.

To further study the interaction of firefly luciferase with its substrate, a student designs an experiment testing the rate at which the molecules involved are recycled. The student gathers 100 fireflies and separates them randomly into five equal experimental groups. Group A is not given any treatment and each subsequent group of fireflies is administered increasing concentrations of luciferin. Each group of fireflies is then released into separate pitch-black rooms that mimic the fireflies’ natural habitat. These rooms also contain light meters that measure the intensity of light emitted by the group of 20 fireflies as a whole. The results of this experiment are shown in Table 1.

Table 1

With which of the following statements would the author most likely agree?