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Example Questions
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
Which of these molecules is not a product of the citric acid cycle?
NADH
Pyruvate
Flavin mononucleotide (FMN)
CO2
Ubiquinol (QH2)
Flavin mononucleotide (FMN)
Flavin mononucleotide (FMN) is not produced by the citric acid cycle. This flavin coenzyme is a reactant, but not a product, since FMN will get reduced to FMNH2.
The rest of the answer choices are products of the citric acid cycle (otherwise known as the Krebs cycle).
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
What is pyruvate converted to before it enters the citric acid cycle?
Acetyl-CoA
Glucose
Acetate
Acetyl-CoA
Pyruvate is the end product of glycolysis. After glycolysis, the three-carbon molecule pyruvate is converted into the two-carbon molecule acetyl-coenzyme A (acetyl-CoA). This is carried out by a combination of three enzymes collectively known as the pyruvate dehydrogenase complex. The conversion of pyruvate to acetyl-CoA also produces one molecule of . Acetyl-CoA has one less carbon than pyruvate. The third carbon from pyruvate is lost as carbon dioxide () during the conversion of pyruvate to acetyl-CoA. Recall that since glucose is a six-carbon molecule, two molecules of pyruvate (three carbons each) are formed via glycolysis.
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
The first reaction of the citric acid cycle is an aldol condensation involving which two molecules?
and pyruvate
and acetyl-CoA
Oxaloacetate and malate
Acetyl-CoA and oxaloacetate
Acetyl-CoA and oxaloacetate
Pyruvate is produced in the last step of glycolysis, then, it is converted to the two-carbon molecule acetyl-coenzyme A (acetyl-CoA). This is carried out by a combination of three enzymes collectively known as the pyruvate dehydrogenase complex. The conversion of pyruvate to acetyl-CoA produces one . Acetyl-CoA has one less carbon than pyruvate. The third carbon of pyruvate is lost as carbon dioxide () during the conversion of pyruvate to acetyl-CoA. The citric acid cycle begins when the four-carbon molecule, oxaloacetate combines with acetyl-CoA (a two carbon molecule) via an aldol condensation, yielding the six-carbon molecule citrate.
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
At what step is produced during the citric acid cycle?
During conversion of citric acid into isocitrate by acontinase
During conversion of succinate into fumarate by succinate dehydrogenase
During conversion of oxaloacetate and acetyl-CoA to form citric acid by citrate synthase
During conversion of succinate into fumarate by succinate dehydrogenase
During conversion of succinate into fumarate by succinate dehydrogenase
During the conversion of succinate into fumarate by succinate dehydrogenase, a single molecule of is reduced to as it accepts the hydrogens from succinate. then feeds its electrons into the electron transport chain in the inner mitochondrial membrane.
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
In the Krebs cycle, which step involves the formation of GTP?
Succinate fumarate
Isocitrate alpha-ketoglutarate
Succinyl-CoA succinate
Alpha-ketoglutarate succinyl-CoA
None of these, GTP is not a product of Krebs cycle
Succinyl-CoA succinate
Succinyl-CoA synthetase performs substrate level phosphorylation at this step in the Krebs cycle, such that .
Example Question #2 : Reactants And Products Of The Citric Acid Cycle
From a single molecule of glucose, two molecules of pyruvate are formed which can be converted into two acetyl-CoA molecules to enter the citric acid cycle.
Starting from a single molecule of glucose, how many molecules of NADH are formed in a single turn of the citric acid cycle including the conversion of pyruvate into acetyl-CoA? Do not include NADH formed during glycolysis, which is not part of the citric acid cycle.
The conversion of pyruvate to acetyl-CoA produces one molecule of NADH, but remember that each glucose yields two pyruvates, so the total NADH from this first step is two. Within the citric acid cycle, there are three steps in which NADH is a byproduct, but again we must remember that each step occurs to two molecules, therefore three NADH byproducts for two molecules yields six NADH in the cycle proper. Therefore, the total NADH produced in one turn of the citric acid cycle is eight NADH.
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
Which of the following molecules are initial reactants of the citric acid cycle?
None of these are initial reactants of citric acid cycle
Pyruvate
NADH
None of these are initial reactants of citric acid cycle
Citric acid cycle inputs are derived from glycolysis outputs. Glycolysis produces pyruvate molecules, , and ATP. The pyruvate molecules undergo reactions that convert the three carbon pyruvate to a two carbon acetyl CoA and an one carbon carbon dioxide. The acetyl-CoA molecules are then used as the initial inputs for the citric acid cycle, as they are combined with oxaloacetate. Note that pyruvate itself does not enter the citric acid cycle. and are electron carriers that are produced in the citric acid cycle and are used in electron transport chain to generate ATP.
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
A glucose molecule entering the cell can indirectly produce __________ and __________ in the citric acid cycle.
one . . . three
two . . . six
six . . . two
three . . . one
six . . . two
A glucose (six carbons) molecule enters glycolysis and produces two three carbon molecules (pyruvate). Each pyruvate is broken down into a two carbon acetyl-CoA molecule that enters the citric acid cycle. Each acetyl-CoA molecule produces three and one in the citric acid cycle. This means that two acetyl-CoA (derived from one glucose molecule) produces six and two molecules in the citric acid cycle.
Example Question #4 : Reactants And Products Of The Citric Acid Cycle
Which of the following is true regarding the citric acid cycle?
Most of the intermediates of this cycle have four more carbons than acetyl-CoA
More than one of these are true
The citric acid cycle produces GTP
The citric acid cycle produces carbon dioxide
More than one of these are true
Citric acid cycle involves a series of reactions that are involved in the production of the necessary molecules for electron transport chain. The cycle starts with a two carbon molecule (acetyl-CoA) binding to a four carbon molecule (oxaloacetate). This creates a six carbon molecule (citrate) that can go through a series of reactions. Most of these reactions involve a six carbon molecule. As mentioned, acetyl-CoA has two carbons; therefore, most of the intermediates in this cycle have six carbons, or four more carbons than acetyl-CoA. One turn of citric acid cycle produces , , (carbon dioxide) and one GTP molecule(s).
Example Question #1 : Reactants And Products Of The Citric Acid Cycle
Which of the following molecules involved in the citric acid cycle initiates the cycle by combining with acetyl-CoA?
Malate
Citrate
Oxaloacetate
Fumarate
Succinate
Oxaloacetate
The first step in the citric acid cycle is for acetyl-CoA to react with oxaloacetate. This forms citrate, which then continues through the cycle, ultimately reforming the oxaloacetate molecule to redo the cycle.
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