There are three tRNA binding sites -- A, P, and E (for Aminoacyl, Peptidyl, and Exit) -- but only one mRNA binding site. The enzyme which bonds amino acids carried by tRNAs at A and P is indeed called peptidyl transferase. tRNA is held at A and P when its anticodon matches the codon of the mRNA to be translated. Because each codon is three codons long, per amino acid, the mRNA is indeed shifted three nucleotides' length through the ribosome, each time an amino acid is added to the growing chain.

Among the amino acids, there are two which only have one codon that code for them: tryptophan (UGG), and methionine. Methionine, is, of course, special among them, because the same codon is also the start codon -- AUG. Aspartic acid, asparagine, tyrosine, and phenylalanine all each have two possible corresponding codons (respectively: GAC/GAU, AAC/AAU, UAC/UAU, and UUC/UUU).

eIF2B is a GEF (guanine nucleotide exchange factor) for eIF1 eIF2. 

eEF1A first binds to the aminoacyl-tRNA and has GTPase activity. eEF1B is a GEF for eEF1A. eEF2 has the elongation role similar to EF-G in prokaryotes. Neither Ran-GTP nor IP3 are elongation factors.

All are part of RNA interference.

siRNA = short interfering RNA

miRNA = micro RNA

pri-miRNA = primary miRNA

piRNA = piwi interacting RNA

Aminoacyl-tRNA synthetases are important enzymes in translation. Their function is to match the specific amino acid to its tRNA. DNA polymerases, RNA polymerase, and DNA helicase are not involved in this process. DNA polymerases are enzymes involved in DNA replication; they create DNA molecules by assembling nucleotides. RNA polymerase produces RNA and has nothing to do with the translation process. Lastly, DNA helicase unwinds DNA during DNA replication, allowing the strands to be copied.

For this question, we're asked to identify an answer choice that contains something that is not needed for transcription and translation.

To begin, let's define these two terms. Transcription is the production of mRNA from DNA. The subsequent coding of a polypeptide from this mRNA is known as translation. During translation, tRNA serves as the carriers of amino acids. In doing so, these tRNA's bring certain amino acids to the ribosome-mRNA complex, depending on the codon sequence of the mRNA. Furthermore, the ribosome itself is composed of rRNA as well as protein. So in total, DNA, mRNA, rRNA, and tRNA are needed for transcription and, subsequently, translation.

But what about miRNA? This type of RNA, together with another class of RNA called siRNA, are both involved in a process called RNA interference. In this process, either miRNA or siRNA acts to inhibit gene expression by inhibiting certain key steps at the level of transcription and translation. Therefore, miRNA is not required for proper transcription and translation to occur because it acts to inhibit these processes.

AUG is the universal start codon. The stop codons are UGA, UAG, and UAA. 

Protein translation begins by recognizing an initiation signal on the mRNA - the codon UAG. The amino acid that coded for by UAG is methionine.

The initiation complex of a ribosome to start translation begins with the tRNA carrying methionine, matching the start codon AUG on mRNA, binding with the 40S and 60S ribosomal subunits, to form an 80S ribosomal subunit with the initiation tRNA in the P site. 

The next tRNA that matches the following codon will then come into the A site to continue translation.