AP Biology : Understanding Bacteria

Study concepts, example questions & explanations for AP Biology

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Example Questions

Example Question #21 : Understanding Bacteria

After a plasmid incorporates outside genes into its own genome, the plasmid may be cloned in which of the following ways?

Possible Answers:

Putting its genome into a bacterium that would begin to replicate the new genetic information

Putting its genome into a virus that would begin to replicate the new genetic information

Undergoing mitosis

Re-programing its DNA to replicate itself

Treating it with a restriction enzyme

Correct answer:

Putting its genome into a bacterium that would begin to replicate the new genetic information

Explanation:

A plasmid replicates itself by being introduced to a bacterium and inserting its genetic information. The bacterium then undergoes its replication process with the genetic information that was injected by the plasmid and in this way the plasmid is cloned. Bacteria do not undergo mitosis, rather, they undergo a process called binary fission to produce identical daughter cells.

Example Question #22 : Understanding Bacteria

Bacteria are susceptible to antibiotics for several reasons. Pharmaceutical companies research new antibiotics continually due to antibiotic resistance.

Characteristics of a good antibiotic include which of the following?

Possible Answers:

Targeting a biological function different than the host, uptake by the bacteria, ease of administration to patient, harsh side effects on the host

Targeting a biological function similar to that of the host, uptake by the bacteria, ease of administration to patient, little to no side effects to the host

Targeting a biological function different than the host, the type of bacteria, ease of administration to patient, little to no side effects to the host

Targeting a biological function different than the host, uptake by the bacteria, the patient's blood type, little to no side effects to the host

Targeting a biological function different than the host, uptake by the bacteria, ease of administration to patient, little to no side effects to the host

Correct answer:

Targeting a biological function different than the host, uptake by the bacteria, ease of administration to patient, little to no side effects to the host

Explanation:

A good antibiotic should target a biological system or function of the bacteria that is different than the host; an example being peptidoglycan in the cell wall of any bacteria. The antibiotic should be broad spectrum, or in other words have the ability to treat several types of bacterial infections regardless of the type of bacteria. The antibiotic should be able to be taken orally or intravenously for ease of administration. In addition most antibiotics are a dry form that can be stored at room temperature or above in order to make it ease to administer in underdeveloped countries or areas of poverty. The side effects should be negligible to the condition. Side effects are in direct proportion to the damage the chemical is doing to the host.  If side effects are to harsh, other complications to the host, including death, could result.

Example Question #22 : Understanding Bacteria

Antibiotics should be used for which of the following pathogens?

Possible Answers:

Candida albicans

West nile virus

All of these

Klebsiella pneumoniae

Common cold

Correct answer:

Klebsiella pneumoniae

Explanation:

Antibiotics should be used to treat bacterial infections only. West Nile Virus is a virus and C. albicans is a fungus. The common cold is caused by an infection by a virus, most often rhinovirus and human coronavirus. K. pneumonia on the other hand, causes pneumonia, and a person will suffer from deep cough and congestion for months or until treated.

Example Question #22 : Microorganisms And Viruses

Which of these is not a characteristic of prokaryotic organisms?

Possible Answers:

Nucleus

Lipid bilayer

Cell wall

Ribosomes

DNA and RNA

Correct answer:

Nucleus

Explanation:

A membrane-bound nucleus is not present in prokaryotic organisms. All other structures can be found in prokaryotes.

Example Question #21 : Microorganisms And Viruses

Antibiotic susceptibility tests were developed by disc method.

What is the zone of inhibition in this method?

Possible Answers:

The growth media under the antibiotic disc

The number of bacteria per milliliter of broth culture spread onto the plate

The area of clearing of the bacteria surrounding the disc

The total area of the petri dish

The periphery where the bacteria is growing

Correct answer:

The area of clearing of the bacteria surrounding the disc

Explanation:

The zone of inhibition is the zone around the disc that the antibiotic killed the bacteria.

Example Question #21 : Understanding Bacteria

A student notices that she can lyse plant cells under a microscope using an enzyme she found in class. She performs some additional experiments and determines that the enzyme is breaking down the cell wall of these cells causing them to lyse. She thinks that this same enzyme could be used to lyse bacterial cells. Will this enzyme work on bacteria? Why or why not?

Possible Answers:

No, because bacteria don't have cell walls

No, because the plant cell wall and bacterial cell what are made up of different molecules

No, because the bacteria are too small for this enzyme to affect it

Yes, because bacteria have cells walls that are made up of the same molecules as plant cell walls

No, because bacteria have cell walls that are made up of chitin

Correct answer:

No, because the plant cell wall and bacterial cell what are made up of different molecules

Explanation:

Bacteria have a cell wall made up of peptidoglycan, while plant cells have a cell wall made up of cellulose. The enzyme used by this student is cleaving the cellulose in the plant cell wall, causing these cells to lyse. This enzyme would not be able to cleave peptidoglycan because the molecules are structurally and chemically different.

Example Question #23 : Microorganisms And Viruses

The lack of a nucleus in bacterial cells allows what two cellular processes to occur in tandem?

Possible Answers:

Transcription and translation

Glycolysis and the Krebs cycle

ATP synthesis and translation

Transcription and protein folding

Translation and protein export

Correct answer:

Transcription and translation

Explanation:

Since DNA is not separated from the cytosol in bacterial cells, nascent messenger RNAs that are being transcribed by RNA polymerase can be bound by ribosomes and translation can be initiated before the entire mRNA is even transcribed. All other processes listed may occur simultaneously in prokaryotic and/or eukaryotic cells.

Example Question #24 : Microorganisms And Viruses

Bacteria normally reproduce asexually by binary fission. However bacteria can directly transfer DNA between each other in a form of sexual reproduction. What is this process called?

Possible Answers:

Crossing over

Meiosis

Transformation

Transduction

Conjugation

Correct answer:

Conjugation

Explanation:

There are three processes in which bacteria can acquire new novel genes/alleles for genes. Transformation happens when bacteria uptake naked DNA from the environment and assemble it into their genome. Transduction happens when a phage transfer DNA from one bacteria to another. Transduction is the direct transfer of DNA from a donor bacteria to a recipient bacteria and is often referred to as "bacterial sex."

Example Question #25 : Microorganisms And Viruses

In eukaryotic cells, the electron transport proteins are located in the inner mitochondrial membrane. Where are they located in bacterial cells?

Possible Answers:

In the cell wall

In the nuclear membrane

In the plasma membrane

In the periplasm

In the cytosol

Correct answer:

In the plasma membrane

Explanation:

Since bacterial cells lack mitochondria, the proteins that make up the electron transport chain reside in the plasma membrane and pump protons from the cytosol into the periplasm. There is no nuclear membrane in bacterial cells since bacteria lack nuclei.

Example Question #26 : Microorganisms And Viruses

In aerobic bacteria, in what part of the cell is the electron transport chain located?

Possible Answers:

The cell wall

The cytosol 

The plasma membrane

The mitochondrial inner membrane

The nucleoid

Correct answer:

The plasma membrane

Explanation:

In the mitochondria of eukaryotic cells, the electron transport chain is located in the inner membrane. Mitochondria are believed to be descended from bacteria that were long ago engulfed by other prokaryotic cells, producing the first eukaryotic cells. The outer membrane of mitochondria can be thought of being derived from the vesicle that was used to engulf the bacterial cell, and the inner membrane can be thought of being derived from the plasma membrane of the original bacterium.

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