All SAT Critical Reading Resources
Example Questions
Example Question #12 : Content Of Natural Science Passages
Adapted from “Humming-Birds: As Illustrating the Luxuriance of Tropical Nature” in Tropical Nature, and Other Essays by Alfred Russel Wallace (1878)
The food of hummingbirds has been a matter of much controversy. All the early writers down to Buffon believed that they lived solely on the nectar of flowers, but since that time, every close observer of their habits maintains that they feed largely, and in some cases wholly, on insects. Azara observed them on the La Plata in winter taking insects out of the webs of spiders at a time and place where there were no flowers. Bullock, in Mexico, declares that he saw them catch small butterflies, and that he found many kinds of insects in their stomachs. Waterton made a similar statement. Hundreds and perhaps thousands of specimens have since been dissected by collecting naturalists, and in almost every instance their stomachs have been found full of insects, sometimes, but not generally, mixed with a proportion of honey. Many of them in fact may be seen catching gnats and other small insects just like fly-catchers, sitting on a dead twig over water, darting off for a time in the air, and then returning to the twig. Others come out just at dusk, and remain on the wing, now stationary, now darting about with the greatest rapidity, imitating in a limited space the evolutions of the goatsuckers, and evidently for the same end and purpose. Mr. Gosse also remarks, ” All the hummingbirds have more or less the habit, when in flight, of pausing in the air and throwing the body and tail into rapid and odd contortions. This is most observable in the Polytmus, from the effect that such motions have on the long feathers of the tail. That the object of these quick turns is the capture of insects, I am sure, having watched one thus engaged pretty close to me.”
What evidence does Mr. Gosse have to support the claim that hummingbirds eat insects?
He observed one flailing around in the air and concluded that it was eating insects.
He surmised that they must eat insects because he has never seen one eating flower nectar.
A hummingbird got into his collection of live insects, and soon after, all of his insects were missing.
He examined the contents of a hummingbird’s stomach and found many insects in it.
He read in a reputable scientific journal that they eat insects.
He observed one flailing around in the air and concluded that it was eating insects.
To answer this question, we have to consider the quotation attributed to Mr. Gosse found at the end of the passage:
“Mr. Gosse also remarks, ‘All the hummingbirds have more or less the habit, when in flight, of pausing in the air and throwing the body and tail into rapid and odd contortions. This is most observable in the Polytmus, from the effect that such motions have on the long feathers of the tail. That the object of these quick turns is the capture of insects, I am sure, having watched one thus engaged pretty close to me.’”
He doesn’t mention anything about having a collection of live insects, getting his information from a scientific journal, or dissecting a hummingbird’s stomach, so we can ignore those answer choices. He actively observes a hummingbird and surmises that they eat insects because of that, so the correct answer is “He observed one flailing around in the air and concluded that it was eating insects.”
Example Question #132 : Narrative Science Passages
Adapted from An Introduction to Astronomy by Forest Ray Moulton (1916 ed.)
It is doubtful if any important scientific idea ever sprang suddenly into the mind of a single man. The great intellectual movements in the world have had long periods of preparation, and often many men were groping for the same truth, without exactly seizing it, before it was fully comprehended.
The foundation on which all science rests is the principle that the universe is orderly, and that all phenomena succeed one another in harmony with invariable laws. Consequently, science was impossible until the truth of this principle was perceived, at least as applied to a limited part of nature.
The phenomena of ordinary observation, as, for example, the weather, depend on such a multitude of factors that it was not easy for men in their primitive state to discover that they occur in harmony with fixed laws. This was the age of superstition, when nature was supposed to be controlled by a great number of capricious gods whose favor could be won by childish ceremonies. Enormous experience was required to dispel such errors and to convince men that the universe is one vast organization whose changes take place in conformity with laws which they can in no way alter.
The actual dawn of science was in prehistoric times, probably in the civilizations that flourished in the valleys of the Nile and the Euphrates. In the very earliest records of these people that have come down to modern times it is found that they were acquainted with many astronomical phenomena and had coherent ideas with respect to the motions of the sun, moon, planets, and stars. It is perfectly clear from their writings that it was from their observations of the heavenly bodies that they first obtained the idea that the universe is not a chaos. Day and night were seen to succeed each other regularly, the moon was found to pass through its phases systematically, the seasons followed one another in order, and in fact the more conspicuous celestial phenomena were observed to occur in an orderly sequence. It is to the glory of astronomy that it first led men to the conclusion that law reigns in the universe.
According to this passage, why were astronomical bodies so important to the emergence of science?
They were once believed to be gods, but by showing that they were not such, humanity was able to believe in scientific thought.
Being above the earth's surface, they seem to encompass the whole of the world.
None of the other answers
Their beauty encouraged continuous speculation.
They exhibit a great deal of regularity.
They exhibit a great deal of regularity.
The key sentence for this question is, "It is perfectly clear from their writings that it was from their observations of the heavenly bodies that they first obtained the idea that the universe is not a chaos." The idea is that for prehistoric humanity, the stars and planets likely provided the first example of regularity in our day-to-day experience. Though many of our experiences seem random, the stars do indeed continue in their courses and the sun has its own repeating path. Hence, they began to see that the world had regular patterns—not all is chaos.
Example Question #36 : Identifying And Analyzing Important Details In Natural Science Passages
Adapted from “Darwin’s Predecessors” by J. Arthur Thomson in Evolution in Modern Thought (1917 ed.)
In seeking to discover Darwin's relation to his predecessors, it is useful to distinguish the various services which he rendered to the theory of organic evolution.
As everyone knows, the general idea of the doctrine of descent is that the plants and animals of the present day are the lineal descendants of ancestors on the whole somewhat simpler, that these again are descended from yet simpler forms, and so on backwards towards the literal "Protozoa" and "Protophyta" about which we unfortunately know nothing. Now no one supposes that Darwin originated this idea, which in rudiment at least is as old as Aristotle. What Darwin did was to make it current intellectual coin. He gave it a form that commended itself to the scientific and public intelligence of the day, and he won widespread conviction by showing with consummate skill that it was an effective formula to work with, a key which no lock refused. In a scholarly, critical, and preeminently fair-minded way, admitting difficulties and removing them, foreseeing objections and forestalling them, he showed that the doctrine of descent supplied a modal interpretation of how our present-day fauna and flora have come to be.
In the second place, Darwin applied the evolution-idea to particular problems, such as the descent of man, and showed what a powerful tool it is, introducing order into masses of uncorrelated facts, interpreting enigmas both of structure and function, both bodily and mental, and, best of all, stimulating and guiding further investigation. But here again it cannot be claimed that Darwin was original. The problem of the descent or ascent of man, and other particular cases of evolution, had attracted not a few naturalists before Darwin's day, though no one [except Herbert Spencer in the psychological domain (1855)] had come near him in precision and thoroughness of inquiry.
In the third place, Darwin contributed largely to a knowledge of the factors in the evolution-process, especially by his analysis of what occurs in the case of domestic animals and cultivated plants, and by his elaboration of the theory of natural selection, which Alfred Russel Wallace independently stated at the same time, and of which there had been a few previous suggestions of a more or less vague description. It was here that Darwin's originality was greatest, for he revealed to naturalists the many different forms—often very subtle—which natural selection takes, and with the insight of a disciplined scientific imagination he realized what a mighty engine of progress it has been and is.
Which of the following is a reason for how Darwin’s ideas provided a powerful tool?
They provided the complete history of life on earth.
They helped to reorganize the sciences along historical lines.
None of the other answers
They gave the most details about how the human species differs from apes.
They enabled scientists to see certain groups of data as single, intelligible wholes.
They enabled scientists to see certain groups of data as single, intelligible wholes.
The key phrase in the passage for this question is "introducing order into masses of uncorrelated facts." The idea is that Darwin's theories provide a tool by enabling this sort of process of data gathering. Uncorrelated facts are ones that have no order (no co-relation to each other). The idea here is that Darwin's insights helped to provide context to such data, helping to organize them. This made the insights into a powerful tool for science (because inquiry is difficult where there is no order at all).
Example Question #12 : Content Of Natural Science Passages
Adapted from “Darwin’s Predecessors” by J. Arthur Thomson in Evolution in Modern Thought (1917 ed.)
In seeking to discover Darwin's relation to his predecessors, it is useful to distinguish the various services which he rendered to the theory of organic evolution.
As everyone knows, the general idea of the doctrine of descent is that the plants and animals of the present day are the lineal descendants of ancestors on the whole somewhat simpler, that these again are descended from yet simpler forms, and so on backwards towards the literal "Protozoa" and "Protophyta" about which we unfortunately know nothing. Now no one supposes that Darwin originated this idea, which in rudiment at least is as old as Aristotle. What Darwin did was to make it current intellectual coin. He gave it a form that commended itself to the scientific and public intelligence of the day, and he won widespread conviction by showing with consummate skill that it was an effective formula to work with, a key which no lock refused. In a scholarly, critical, and preeminently fair-minded way, admitting difficulties and removing them, foreseeing objections and forestalling them, he showed that the doctrine of descent supplied a modal interpretation of how our present-day fauna and flora have come to be.
In the second place, Darwin applied the evolution-idea to particular problems, such as the descent of man, and showed what a powerful tool it is, introducing order into masses of uncorrelated facts, interpreting enigmas both of structure and function, both bodily and mental, and, best of all, stimulating and guiding further investigation. But here again it cannot be claimed that Darwin was original. The problem of the descent or ascent of man, and other particular cases of evolution, had attracted not a few naturalists before Darwin's day, though no one [except Herbert Spencer in the psychological domain (1855)] had come near him in precision and thoroughness of inquiry.
In the third place, Darwin contributed largely to a knowledge of the factors in the evolution-process, especially by his analysis of what occurs in the case of domestic animals and cultivated plants, and by his elaboration of the theory of natural selection, which Alfred Russel Wallace independently stated at the same time, and of which there had been a few previous suggestions of a more or less vague description. It was here that Darwin's originality was greatest, for he revealed to naturalists the many different forms—often very subtle—which natural selection takes, and with the insight of a disciplined scientific imagination he realized what a mighty engine of progress it has been and is.
Based on this passage, what was the role of Herbert Spencer in the history of evolutionary doctrine?
None of the other answers
His thought had aspects related to evolution as a theme in certain social sciences.
His thought had no major relation to thought pertaining to evolution.
In many ways, his thought presaged that of Darwin's, predicting several major aspects of Darwin's theories.
He was the only real source from which Darwin drew his thought.
His thought had aspects related to evolution as a theme in certain social sciences.
In the passage, it is said that few naturalists focused on evolution or the descent/ascent of man before Darwin's day. "Naturalists" would be "natural scientists." The parenthetical remark about Spencer makes sure that the reader knows, however, that Spencer did pay attention to it "in the psychological domain." Some argue that he was influential to Darwin, but that is not discussed here. However, we can say that his thought did contain aspects that applied the idea of evolution to certain branches of the social sciences.
Example Question #32 : Identifying And Analyzing Important Details In Natural Science Passages
"Cacti" by Ami Dave (2013)
Cacti are plants suited to the desert, and we must always keep this factor in mind when growing ornamental cacti in our gardens, for it helps us provide cacti with conditions that allow them to survive and thrive. For example, a cactus should never be watered over its body, as it will start to rot. This is because it is covered with a waxy coating which prevents water loss through evaporation. When one waters the cactus over its body, the waxy coating is washed away and the plant begins to rot. The amount of water that one must supply to the cactus is very much dependent upon the season and upon the climate of the place. During the summer season one should water cacti every four days, whereas in the rainy season, once every fifteen days is quite enough.
Cacti need a minimum of two and a half hours of sunlight per day; however, they should not be kept in the sun all day because they may wrinkle when exposed to too much bright sunlight. Unlike other plants, cacti produce carbon dioxide during the day and oxygen during the night, so they are ideal plants to be kept in bedrooms to freshen up the air at night.
If a cactus is to thrive and prosper, the size of the pot in which it is grown needs to be monitored carefully. The pot should always be a little smaller than the plant itself because it is only when the plant has to struggle to survive that it will thrive. If the pot is too spacious and the plant does not need to struggle, chances are that the cactus will die. Similarly, if a cactus shows no signs of growth, stop watering it. Watering should be resumed only when the plant begins to grow again.
The substrata of a cactus pot is ideally composed of pieces of broken bricks at the bottom, followed by a layer of charcoal above the bricks, and then coarse sand and pebbles above the charcoal. Leaf mould is the best manure.
Grafting cacti is very simple. A very small piece of the cactus plant should be stuck with tape to the plant that needs grafting. The smaller the piece, the easier it is to graft. To reproduce cacti, one has to simply cut off a piece of the cactus, allow it to dry for a few days, and then place it over the cacti substrate. It will automatically develop roots.
It is very easy to differentiate between cacti and other plants that look like cacti. All cacti have fine hair at the base of each thorn. The so-called “thorns” are in fact highly modified leaves which prevent loss of water through transpiration. If one ever gets pricked by cacti thorns, one should take tape, place it over the area where the thorns have penetrated the skin, and then peel it off. All of the thorns will get stuck to the tape and will be removed.
The first paragraph provides all of the following information EXCEPT __________.
cacti are best suited to the desert
frequency of watering should vary based on the season
cacti can rot if watered incorrectly
cacti lack an outer coating to protect them from water loss
watering the body of the cactus can be harmful to the plant
cacti lack an outer coating to protect them from water loss
The passage states the opposite of the answer choice "it is covered with a waxy coating that prevents water loss through evaporation." The rest of the answer choices are all correct information, which is provided in the first paragraph.
Example Question #31 : Identifying And Analyzing Important Details In Natural Science Passages
"Cacti" by Ami Dave (2013)
Cacti are plants suited to the desert, and we must always keep this factor in mind when growing ornamental cacti in our gardens, for it helps us provide cacti with conditions that allow them to survive and thrive. For example, a cactus should never be watered over its body, as it will start to rot. This is because it is covered with a waxy coating which prevents water loss through evaporation. When one waters the cactus over its body, the waxy coating is washed away and the plant begins to rot. The amount of water that one must supply to the cactus is very much dependent upon the season and upon the climate of the place. During the summer season one should water cacti every four days, whereas in the rainy season, once every fifteen days is quite enough.
Cacti need a minimum of two and a half hours of sunlight per day; however, they should not be kept in the sun all day because they may wrinkle when exposed to too much bright sunlight. Unlike other plants, cacti produce carbon dioxide during the day and oxygen during the night, so they are ideal plants to be kept in bedrooms to freshen up the air at night.
If a cactus is to thrive and prosper, the size of the pot in which it is grown needs to be monitored carefully. The pot should always be a little smaller than the plant itself because it is only when the plant has to struggle to survive that it will thrive. If the pot is too spacious and the plant does not need to struggle, chances are that the cactus will die. Similarly, if a cactus shows no signs of growth, stop watering it. Watering should be resumed only when the plant begins to grow again.
The substrata of a cactus pot is ideally composed of pieces of broken bricks at the bottom, followed by a layer of charcoal above the bricks, and then coarse sand and pebbles above the charcoal. Leaf mould is the best manure.
Grafting cacti is very simple. A very small piece of the cactus plant should be stuck with tape to the plant that needs grafting. The smaller the piece, the easier it is to graft. To reproduce cacti, one has to simply cut off a piece of the cactus, allow it to dry for a few days, and then place it over the cacti substrate. It will automatically develop roots.
It is very easy to differentiate between cacti and other plants that look like cacti. All cacti have fine hair at the base of each thorn. The so-called “thorns” are in fact highly modified leaves which prevent loss of water through transpiration. If one ever gets pricked by cacti thorns, one should take tape, place it over the area where the thorns have penetrated the skin, and then peel it off. All of the thorns will get stuck to the tape and will be removed.
The passage addresses all of the following EXCEPT __________.
ideal pot size
how to tell cacti apart from other plants
how often to water a cactus
what to do when pricked by a cactus thorn
how water flows through a cactus from the roots
how water flows through a cactus from the roots
The passage addresses that watering the body of the cactus can have harmful effects, and therefore one should water the roots only; however, it does not discuss the biological mechanism behind how the cactus transports water upward to the body (against the force of gravity).
Example Question #21 : Drawing Evidence From Natural Science Passages
Adapted from “Birds in Retreat” in “Animal Defences—Active Defence” in Volume Four of The Natural History of Animals: The Animal Life of the World in Its Various Aspects and Relations by James Richard Ainsworth Davis (1903)
Among the large running birds are forms, like the African ostrich, in which the absence of powers of flight is largely compensated by the specialization of the legs for the purpose of rapid movement on the ground. For straightforward retreat in open country nothing could be more effective; but another kind of adaptation is required in birds like rails, which are deficient in powers of flight, and yet are able to run through thickly-growing vegetation with such rapidity as to commonly elude their enemies. This is rendered possible by the shape of their bodies, which are relatively narrow and flattened from side to side, so as to easily slip between the stems of grasses, rushes, and similar plants. Anyone who has pursued our native land-rail or corn-crake with intent to capture will have noted how extremely difficult it is even to get within sight of a bird of this sort.
Certain birds, unfortunately for themselves, have lost the power of flight without correspondingly increased powers of running, and have paid the penalty of extinction. Such an arrangement, as might be anticipated, was the result of evolution in islands devoid of any predatory ground-animals, and a classic example of it is afforded by the dodo and its allies, birds related to the pigeons. The dodo itself was a large and clumsy-looking species that at one time abounded in the island of Mauritius, which, like oceanic islands generally, possessed no native mammals, while its indigenous reptiles were only represented by lizards. The ubiquitous sailor, however, and the animals (especially swine) which he introduced, brought about the extinction of this helpless bird in less than a century after its first discovery in 1598. Its memory is now only kept green by a few contemporary drawings and descriptions, certain museum remains, and the proverb "as extinct as a dodo.” A similar fate must overtake any organism suddenly exposed to new and unfavorable conditions, if devoid of sufficient plasticity to rapidly accommodate itself to the altered environment.
What kinds of reptiles are indigenous to Mauritius?
Snakes
Snakes and crocodiles
Lizards and snakes too small to eat dodos
Lizards
Lizards, snakes, and crocodiles
Lizards
The passage introduces Mauritius as “the island of Mauritius, which, like oceanic islands generally, possessed no native mammals, while its indigenous reptiles were only represented by lizards.” This tells us that the only reptiles indigenous to Mauritius are lizards, making “lizards” the correct answer.
Example Question #72 : Natural Science Passages
Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)
As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.
As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.
Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.
Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.
The passage states that which of the following is true?
The interference of Thrips causes a great effect on the results of the experiment.
It was impossible to eliminate many of the occurring errors.
Darwin only considered the large but unmeasured amount of pollen he was placing on each flower's stigma to be a potential variable in his experiment after two seasons.
The seeds were allowed to germinate on the top of soil.
Self-sterile plants often become fertilised when placed in close proximity with one another.
Darwin only considered the large but unmeasured amount of pollen he was placing on each flower's stigma to be a potential variable in his experiment after two seasons.
In the final paragraph, Darwin writes, "I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious." So, Darwin only considered the large but unmeasured amount of pollen that he placed on flowers' stigmas to be a potential variable in his experiment "after having acted in this manner during two seasons." If you chose the answer "The seeds were allowed to germinate on the top of soil," then it is important to note that the seeds were germinated on sand, not earth or soil, as it says in the third and fourth paragraphs near the end of each.
Example Question #154 : Sat Critical Reading
Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)
As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.
As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.
Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.
Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.
The last paragraph establishes all of the following EXCEPT __________.
Gartner believed that more pollen was harmful to fertilization without cause.
The under-pollinated flowers yielded more capsules.
For two seasons, the author neglected to give exactly equal amounts of pollen to the flowers.
Overall, the over-pollinated seeds produced sixty five capsules.
Experiments were conducted to verify variables.
Overall, the over-pollinated seeds produced sixty five capsules.
The author states that overall the sixty-four flowers which were given more pollen produced sixty-one capsules, of which four were not used in the calculation of averages due to their contents being of poor quality.
Example Question #32 : Natural Sciences
Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)
As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.
As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.
Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.
Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.
Which of the following statements about the plants is supported by the passage?
The plants were raised by choice in proximity to insects.
Meshes were used during the growing period.
To protect the plants, they were placed in greenhouses.
In each experiment, the plants were all variants of the same species.
The plants were recommended by another scientist.
Meshes were used during the growing period.
During the discussion of Thrips in the first paragraph, the author mentions the use of nets, which can be interpreted as protective meshes, to protect the plants: “several plants of the same species were placed under the same net.” There is no mention in the text of the use of variants.