"The Cell Cycle" by Joseph Ritchie (2014)

The process by which cells divide and multiply is known as the cell cycle. This cycle consists of two main phases: interphase and mitosis. Each phase consists of a series of clearly defined and observable steps. At the conclusion of the cycle, each parent cell produces two genetically identical daughter cells that may also replicate by proceeding through the cell cycle.

Roughly ninety percent of the cell cycle is spent in interphase. Interphase is comprised of three main steps: the first gap phase, the synthesis phase (also called "S phase"), and the second gap phase. The initial gap phase is a period of cellular preparation in which the cell increases in size and readies itself for DNA synthesis. In the synthesis phase, or S phase, DNA replication occurs, so that when the cell divides, each daughter cell will have the DNA necessary to function properly. In the second gap phase, the cell grows in size and prepares for cellular division in the mitotic phase. At the end of each gap phase, the cell has to pass a regulatory checkpoint to ensure that nothing is going wrong. If anything has gone wrong, the checkpoints stop the cell from proceeding through the cell cycle any further.

The next part of the cell cycle is mitosis. Mitosis is a form of cell division and is broken down into five distinct phases. During prophase, the genetic material contained in the cell’s chromatin condenses into distinct chromosomes. Prometaphase is marked by the breakdown of the cell’s nuclear envelope and the formation of centrosomes at the poles of the cell. During metaphase, the cell’s chromosomes are moved to the center of the cell. A checkpoint ensures that the chromosomes are properly aligned on the center and halts the cell cycle if any errors have occurred. In anaphase, chromosomes break apart at their center, or centromere, and sister chromatids move to opposite ends of the cell. Lastly, telophase and cytokinesis occur as nuclear membranes form to physically divide the cell into two new daughter cells. Chromosomes also unwind into loose chromatin during this part of mitosis. Cytokinesis is defined as the division of the each cell’s cytoplasm and organelles. At the conclusion of the cell cycle, two genetically identical daughter cells have formed.

The cell cycle operates by a series of checkpoints and external cues. This system of checks enables the cell to enter a state of dormancy known as the gap zero phase when conditions or other factors inhibit the cell cycle. Conversely, unregulated and uncontrolled cellular division can occur under certain circumstances. A cell in a state of uncontrolled division is known to be cancerous. Lastly, cells have the ability to mediate their own death by way of apoptosis if certain genetic or physical abnormalities exist. The cell cycle is a complex process that enables cells to replicate and proliferate under a stringent set of checks and balances that produce healthy and viable daughter cells that are each able to perform the process in the future.

Cytokinesis is best defined as which of the following?

Adapted from Volume Four of The Natural History of Animals: The Animal Life of the World in Its Various Aspects and Relations by James Richard Ainsworth Davis (1903)

The examples of protective resemblance so far quoted are mostly permanent adaptations to one particular sort of surrounding. There are, however, numerous animals which possess the power of adjusting their color more or less rapidly so as to harmonize with a changing environment.

Some of the best known of these cases are found among those mammals and birds that inhabit countries more or less covered with snow during a part of the year. A good instance is afforded by the Irish or variable hare, which is chiefly found in Ireland and Scotland. In summer, this looks very much like an ordinary hare, though rather grayer in tint and smaller in size, but in winter it becomes white with the exception of the black tips to the ears. Investigations that have been made on the closely allied American hare seem to show that the phenomenon is due to the growth of new hairs of white hue. 

The common stoat is subject to similar color change in the northern parts of its range. In summer it is of a bright reddish brown color with the exception of the under parts, which are yellowish white, and the end of the tail, which is black. But in winter, the entire coat, save only the tip of the tail, becomes white, and in that condition the animal is known as an ermine. A similar example is afforded by the weasel. The seasonal change in the vegetarian Irish hare is purely of protective character, but in such an actively carnivorous creature as a stoat or weasel, it is aggressive as well, rendering the animal inconspicuous to its prey.

Which of the following terms is closest in meaning to the underlined word “inconspicuous”?

Adapted from “Feathers of Sea Birds and Wild Fowl for Bedding” from The Utility of Birds by Edward Forbush (ed. 1922)

In the colder countries of the world, the feathers and down of waterfowl have been in great demand for centuries as filling for beds and pillows. Such feathers are perfect non-conductors of heat, and beds, pillows, or coverlets filled with them represent the acme of comfort and durability. The early settlers of New England saved for such purposes the feathers and down from the thousands of wild-fowl which they killed, but as the population increased in numbers, the quantity thus furnished was insufficient, and the people sought a larger supply in the vast colonies of ducks and geese along the Labrador coast. 

The manner in which the feathers and down were obtained, unlike the method practiced in Iceland, did not tend to conserve and protect the source of supply. In Iceland, the people have continued to receive for many years a considerable income by collecting eider down, but there they do not “kill the goose that lays the golden eggs.” Ducks line their nests with down plucked from their own breasts and that of the eider is particularly valuable for bedding. In Iceland, these birds are so carefully protected that they have become as tame and unsuspicious as domestic fowls In North America. Where they are constantly hunted they often conceal their nests in the midst of weeds or bushes, but in Iceland, they make their nests and deposit their eggs in holes dug for them in the sod. A supply of the ducks is maintained so that the people derive from them an annual income.

In North America, quite a different policy was pursued. The demand for feathers became so great in the New England colonies about the middle of the eighteenth century that vessels were fitted out there for the coast of Labrador for the express purpose of securing the feathers and down of wild fowl. Eider down having become valuable and these ducks being in the habit of congregating by thousands on barren islands of the Labrador coast, the birds became the victims of the ships’ crews. As the ducks molt all their primary feathers at once in July or August and are then quite incapable of flight and the young birds are unable to fly until well grown, the hunters were able to surround the helpless birds, drive them together, and kill them with clubs. Otis says that millions of wildfowl were thus destroyed and that in a few years their haunts were so broken up by this wholesale slaughter and their numbers were so diminished that feather voyages became unprofitable and were given up. 

This practice, followed by the almost continual egging, clubbing, shooting, etc. by Labrador fishermen, may have been a chief factor in the extinction of the Labrador duck, that species of supposed restricted breeding range. No doubt had the eider duck been restricted in its breeding range to the islands of Labrador, it also would have been exterminated long ago.

Based on the context in which it is used in the first paragraph, what is the meaning of the underlined word “acme”?

Adapted from “Feathers of Sea Birds and Wild Fowl for Bedding” from The Utility of Birds by Edward Forbush (ed. 1922)

In the colder countries of the world, the feathers and down of waterfowl have been in great demand for centuries as filling for beds and pillows. Such feathers are perfect non-conductors of heat, and beds, pillows, or coverlets filled with them represent the acme of comfort and durability. The early settlers of New England saved for such purposes the feathers and down from the thousands of wild-fowl which they killed, but as the population increased in numbers, the quantity thus furnished was insufficient, and the people sought a larger supply in the vast colonies of ducks and geese along the Labrador coast. 

The manner in which the feathers and down were obtained, unlike the method practiced in Iceland, did not tend to conserve and protect the source of supply. In Iceland, the people have continued to receive for many years a considerable income by collecting eider down, but there they do not “kill the goose that lays the golden eggs.” Ducks line their nests with down plucked from their own breasts and that of the eider is particularly valuable for bedding. In Iceland, these birds are so carefully protected that they have become as tame and unsuspicious as domestic fowls In North America. Where they are constantly hunted they often conceal their nests in the midst of weeds or bushes, but in Iceland, they make their nests and deposit their eggs in holes dug for them in the sod. A supply of the ducks is maintained so that the people derive from them an annual income.

In North America, quite a different policy was pursued. The demand for feathers became so great in the New England colonies about the middle of the eighteenth century that vessels were fitted out there for the coast of Labrador for the express purpose of securing the feathers and down of wild fowl. Eider down having become valuable and these ducks being in the habit of congregating by thousands on barren islands of the Labrador coast, the birds became the victims of the ships’ crews. As the ducks molt all their primary feathers at once in July or August and are then quite incapable of flight and the young birds are unable to fly until well grown, the hunters were able to surround the helpless birds, drive them together, and kill them with clubs. Otis says that millions of wildfowl were thus destroyed and that in a few years their haunts were so broken up by this wholesale slaughter and their numbers were so diminished that feather voyages became unprofitable and were given up. 

This practice, followed by the almost continual egging, clubbing, shooting, etc. by Labrador fishermen, may have been a chief factor in the extinction of the Labrador duck, that species of supposed restricted breeding range. No doubt had the eider duck been restricted in its breeding range to the islands of Labrador, it also would have been exterminated long ago.

Based on the context in which it is used, what is the most likely meaning of the underlined word “egging” in the passage’s last paragraph?

Adapted from "Taking a Second Look: An Analysis of Genetic Markers in Species Relatedness" by Joseph Ritchie (2014)

Phylogenetics is the study of genetic composition in various species and is used by evolutionary biologists to investigate similarities in the molecular sequences of proteins in varying organisms. The amino acid sequences that build proteins are used to construct mathematical matrices that aid in determining evolutionary ties through the investigation of percentage similarities. The study of these matrices helps to expose evolutionary relationships between species that may not have the same overt characteristics.

Species adapt and evolve based on the pressures that exist in their environment. Climate, food source, and habitat availability are only a few factors that act on species adaptation. These stressors can alter the physical characteristics of organisms. This divergence in evolution has made it difficult to determine the interrelatedness of organisms by analyzing their physical characteristics alone.

For instance, looking only at physical characteristics, the ghost bat resembles a pigeon more than a spider monkey; however, phylogenetics has found that the amino acid sequences that construct the beta hemoglobin molecules of bats are twenty percent more similar to those of mammalian primates than those of birds. This helps reject the assumption that common physical characteristics between species are all that is needed to determine relatedness. 

The differences produced by divergent evolution observed in the forest-dwelling, arboreal spider monkey and the nocturnal, airborne ghost bat can be reconciled through homology. Homologous characteristics are anatomical traits that are similar in two or more different species. For instance, the bone structure of a spider monkey’s wrist and fingers greatly resembles that of a bat’s wing or even a whale’s fin. These similarities are reinforced by phylogenetic evidence that supports the idea that physically dissimilar species can be evolutionarily related through anatomical and genetic similarities.

The underlined word "divergent" in paragraph four most nearly means which of the following?

Adapted from "Taking a Second Look: An Analysis of Genetic Markers in Species Relatedness" by Joseph Ritchie (2014)

Phylogenetics is the study of genetic composition in various species and is used by evolutionary biologists to investigate similarities in the molecular sequences of proteins in varying organisms. The amino acid sequences that build proteins are used to construct mathematical matrices that aid in determining evolutionary ties through the investigation of percentage similarities. The study of these matrices helps to expose evolutionary relationships between species that may not have the same overt characteristics.

Species adapt and evolve based on the pressures that exist in their environment. Climate, food source, and habitat availability are only a few factors that act on species adaptation. These stressors can alter the physical characteristics of organisms. This divergence in evolution has made it difficult to determine the interrelatedness of organisms by analyzing their physical characteristics alone.

For instance, looking only at physical characteristics, the ghost bat resembles a pigeon more than a spider monkey; however, phylogenetics has found that the amino acid sequences that construct the beta hemoglobin molecules of bats are twenty percent more similar to those of mammalian primates than those of birds. This helps reject the assumption that common physical characteristics between species are all that is needed to determine relatedness. 

The differences produced by divergent evolution observed in the forest-dwelling, arboreal spider monkey and the nocturnal, airborne ghost bat can be reconciled through homology. Homologous characteristics are anatomical traits that are similar in two or more different species. For instance, the bone structure of a spider monkey’s wrist and fingers greatly resembles that of a bat’s wing or even a whale’s fin. These similarities are reinforced by phylogenetic evidence that supports the idea that physically dissimilar species can be evolutionarily related through anatomical and genetic similarities.

The underlined word "overt" in paragraph one most nearly means which of the following?

Adapted from Ice-Caves of France and Switzerland by George Forrest Browne (1865)

This account states that the cave is in the county of Thorn, among the lowest spurs of the Carpathians. The entrance, which faces the north, and is exposed to the cold winds from the snowy part of the Carpathian range, is eighteen fathoms high and nine broad; and the cave spreads out laterally, and descends to a point fifty fathoms below the entrance, where it is twenty-six fathoms in breadth, and of irregular height. Beyond this no one had at that time penetrated, on account of the unsafe footing, although many distant echoes were returned by the farther recesses of the cave; indeed, to get even so far as this, much step-cutting was necessary.

When the external frost of winter comes on, the account proceeds, the effect in the cave is the same as if fires had been lighted there: the ice melts, and swarms of flies and bats and hares take refuge in the interior from the severity of the winter. As soon as spring arrives, the warmth of winter disappears from the interior, water exudes from the roof and is converted into ice, while the more abundant supplies which pour down on to the sandy floor are speedily frozen there. In the dog-days, the frost is so intense that a small icicle becomes in one day a huge mass of ice; but a cool day promptly brings a thaw, and the cave is looked upon as a barometer, not merely feeling, but also presaging, the changes of weather. The people of the neighborhood, when employed in field-work, arrange their labour so that the mid-day meal may be taken near the cave, when they either ice the water they have brought with them, or drink the melted ice, which they consider very good for the stomach. It had been calculated that six hundred weekly carts would not be sufficient to keep the cavern free from ice. The ground above the cave is peculiarly rich in grass.

In explanation of these phenomena, Bell threw out the following suggestions, which need no comment. The earth being of itself cold and damp, the external heat of the atmosphere, by partially penetrating into the ground, drives in this native cold to the inner parts of the earth, and makes the cold there more dense. On the other hand, when the external air is cold, it draws forth towards the surface the heat there may be in the inner part of the earth, and thus makes caverns warm. In support and illustration of this view, he states that in the hotter parts of Hungary, when the people wish to cool their wine, they dig a hole two feet deep, and place in it the flagon of wine, and, after filling up the hole again, light a blazing fire upon the surface, which cools the wine as if the flagon had been laid in ice. He also suggests that possibly the cold winds from the Carpathians bring with them imperceptible particles of snow, which reach the water of the cave, and convert it into ice. Further, the rocks of the Carpathians abound in salts, niter, alum, etc., which may, perhaps, mingle with such snowy particles, and produce the ordinary effect of the snow and salt in the artificial production of ice.

What is the main idea of the underlined sentence in the second paragraph?

Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)

As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.

As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.

Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.

Which of the following sentences best summarizes the start of the second paragraph?

Adapted from A Practical Treatise on the Hive and Honey-Bee by Lorenzo Lorraine Langstroth (1857 ed.)

Of all the numerous enemies of the honey-bee, the Bee-Moth (Tinea mellonella), in climates of hot summers, is by far the most to be dreaded. So widespread and fatal have been its ravages in this country that thousands have abandoned the cultivation of bees in despair, and in districts which once produced abundant supplies of the purest honey, bee-keeping has gradually dwindled down into a very insignificant pursuit. Contrivances almost without number have been devised to defend the bees against this invidious foe, but still it continues its desolating inroads, almost unchecked, laughing as it were to scorn at all the so-called "moth-proof" hives, and turning many of the ingenious fixtures designed to entrap or exclude it into actual aids and comforts in its nefarious designs.

I should feel but little confidence in being able to reinstate bee-keeping in our country into a certain and profitable pursuit if I could not show the apiarian in what way he can safely bid defiance to the pestiferous assaults of this, his most implacable enemy. I have patiently studied its habits for years, and I am at length able to announce a system of management founded upon the peculiar construction of my hives, which will enable the careful bee-keeper to protect his colonies against the monster. The bee-moth infects our apiaries, just as weeds take possession of a fertile soil. Before explaining the means upon which I rely to circumvent the moth, I will first give a brief description of its habits.

Swammerdam, towards the close of the seventeenth century, gave a very accurate description of this insect, which was then called by the very expressive name of the "bee-wolf." He has furnished good drawings of it, in all its changes, from the worm to the perfect moth, together with the peculiar webs or galleries that it constructs and from which the name of Tinea galleria or “gallery moth” has been given to it by some entomologists. He failed, however, to discriminate between the male and female, which, because they differ so much in size and appearance, he supposed to be two different species of the wax-moth. It seems to have been a great pest in his time, and even Virgil speaks of the "dirum tineæ genus," the dreadful offspring of the moth; that is the worm.

This destroyer usually makes its appearance about the hives in April or May, the time of its coming depending upon the warmth of the climate or the forwardness of the season. It is seldom seen on the wing (unless startled from its lurking place about the hive) until towards dark, and is evidently chiefly nocturnal in its habits. In dark cloudy days, however, I have noticed it on the wing long before sunset, and if several such days follow in succession, the female, oppressed with the urgent necessity of laying her eggs, may be seen endeavoring to gain admission to the hives. The female is much larger than the male, and "her color is deeper and more inclining to a darkish gray, with small spots or blackish streaks on the interior edge of her upper wings." The color of the male inclines more to a light gray; they might easily be mistaken for different species of moths. These insects are surprisingly agile, both on foot and on the wing. The motions of a bee are very slow in comparison. "They are," says Reaumur, "the most nimble-footed creatures that I know." "If the approach to the apiary be observed of a moonlight evening, the moths will be found flying or running round the hives, watching an opportunity to enter, whilst the bees that have to guard the entrances against their intrusion will be seen acting as vigilant sentinels, performing continual rounds near this important post, extending their antenna to the utmost, and moving them to the right and left alternately. Woe to the unfortunate moth that comes within their reach!" "It is curious," says Huber, "to observe how artfully the moth knows how to profit, to the disadvantage of the bees, which require much light for seeing objects; and the precautions taken by the latter in reconnoitering and expelling so dangerous an enemy."

Which of these most accurately restates the meaning of “The bee-moth infects our apiaries, just as weeds take possession of a fertile soil," a line found in the second paragraph?

Adapted from “Darwin’s Predecessors” by J. Arthur Thomson in Evolution in Modern Thought (1917 ed.)

In seeking to discover Darwin's relation to his predecessors, it is useful to distinguish the various services which he rendered to the theory of organic evolution.

As everyone knows, the general idea of the doctrine of descent is that the plants and animals of the present day are the lineal descendants of ancestors on the whole somewhat simpler, that these again are descended from yet simpler forms, and so on backwards towards the literal "Protozoa" and "Protophyta" about which we unfortunately know nothing. Now no one supposes that Darwin originated this idea, which in rudiment at least is as old as Aristotle. What Darwin did was to make it current intellectual coin. He gave it a form that commended itself to the scientific and public intelligence of the day, and he won widespread conviction by showing with consummate skill that it was an effective formula to work with, a key which no lock refused. In a scholarly, critical, and preeminently fair-minded way, admitting difficulties and removing them, foreseeing objections and forestalling them, he showed that the doctrine of descent supplied a modal interpretation of how our present-day fauna and flora have come to be.

In the second place, Darwin applied the evolution-idea to particular problems, such as the descent of man, and showed what a powerful tool it is, introducing order into masses of uncorrelated facts, interpreting enigmas both of structure and function, both bodily and mental, and, best of all, stimulating and guiding further investigation. But here again it cannot be claimed that Darwin was original. The problem of the descent or ascent of man, and other particular cases of evolution, had attracted not a few naturalists before Darwin's day, though no one [except Herbert Spencer in the psychological domain (1855)] had come near him in precision and thoroughness of inquiry.

In the third place, Darwin contributed largely to a knowledge of the factors in the evolution-process, especially by his analysis of what occurs in the case of domestic animals and cultivated plants, and by his elaboration of the theory of natural selection, which Alfred Russel Wallace independently stated at the same time, and of which there had been a few previous suggestions of a more or less vague description. It was here that Darwin's originality was greatest, for he revealed to naturalists the many different forms—often very subtle—which natural selection takes, and with the insight of a disciplined scientific imagination he realized what a mighty engine of progress it has been and is.

What is meant by the underlined expression, “current intellectual coin”?