MCAT Biology : Microbiology

Study concepts, example questions & explanations for MCAT Biology

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Example Questions

Example Question #41 : Microbiology

Which of the following types of bacterial reproduction is most similar to mitosis?

Possible Answers:

Binary fission

Conjugation

Transformation

Transduction

Correct answer:

Binary fission

Explanation:

Mitosis is a form of asexual reproduction in which genetic recombination does not take place; the daughter cells are identical to the parent cell. Prokaryotes are able to divide asexually using binary fission, producing two exact copies of the parent cell. The mechanisms governing binary fission are quite different from those governing mitosis, but the end result is the same.

The other three options are examples in which prokaryotic genetic recombination takes place. Conjugation involves the transfer of genetic material between two bacteria via a sex pilus. Transduction is the transfer of genetic material to a bacterium via a vector, such as a bacteriophage. Transformation occurs when the bacterium uptakes exogenous DNA from the environment.

Example Question #42 : Microbiology

A microbiologist sees that a certain strain of E. coli grew in population size from 20,000 cells to 160,000 cells in two hours. How long would it take this strain to undergo 15 doubling cycles?

Possible Answers:

20hr

5h

30h

600hr

10hr

Correct answer:

10hr

Explanation:

We must first find out how long a doubling cycle is for this strain.  We can determine the following relationships from the information in the question.

So, in two hours, the bacteria has undergone three doubling cycles from 20000 to 160000. That means the doubling time is one-third of two hours, or one-third of 120 minutes.

 

The doubling time is 40 minutes.

The bacteria will undergo 15 doubling cycles in 600 minutes, or ten hours.

Example Question #1 : Bacteria And Types Of Prokaryotes

All of the following are characteristics of prokaryotic cells except for __________.

Possible Answers:

lack of a nucleus

lack of membrane-bound organelles

they are usually found in protists and fungi

unicellularity

a cell wall

Correct answer:

they are usually found in protists and fungi

Explanation:

Protists, fungi, plants, and animals are eukaryotic, while bacteria are prokaryotic. The rest of the answer choices are characteristic of prokaryotic cells.

Example Question #2 : Bacteria And Types Of Prokaryotes

The presence of which of the following feature would best indicate a eukaryotic cell?

Possible Answers:

Homeostasis

Plasma membrane

Ribosomes

Cilia

Organelles

Correct answer:

Organelles

Explanation:

Organelles are only found in eukaryotic cells which set them apart from prokaryotic cells.

Example Question #2 : Bacteria And Types Of Prokaryotes

Which of the following components of an animal cell would NOT also be observed in a bacterial cell?

Possible Answers:

Nucleus

Ribosomes

DNA

Phospholipid cell membrane

 

Correct answer:

Nucleus

Explanation:

This question is asking about one of the differences between eukaryotes and prokaryotes.  While both eukaryotes and prokaryotes use DNA to encode their genetic information, making "DNA" incorrect, remember that prokaryotes lack membrane-bound organelles.  \This makes both "ribosomes" and "phospholipid cell membrane" incorrect, because neither of them are membrane-bound organelles. We are left with "nucleus," which is the correct answer.

Example Question #2 : Bacteria And Types Of Prokaryotes

Which of the following would not be found in a prokaryotic cell?

Possible Answers:

DNA polymerase

Plasma membrane

Mitochondria

RNA

Ribosomes

Correct answer:

Mitochondria

Explanation:

One of the key differences between prokaryotes and eukaryotes is that prokaryotes have no membrane bound organelles, therefore, prokaryotes would not have mitochondria. Prokaryotes have a plasma membrane for protection, ribosomes for protein production, RNA in the form of mRNA, tRNA, and rRNA, and DNA polymerase to replicate their DNA.

Example Question #3 : Bacteria And Types Of Prokaryotes

A scientist studying a culture of bacteria finds an organism he hadn't seen before. He thinks it might be some kind of single-celled eukaryote. What observed characteristic would support the scientists prediction?

Possible Answers:

The presence of ribosomes

An electron transport chain

The presence of both DNA and RNA

A cell wall

A membrane bound organelle

Correct answer:

A membrane bound organelle

Explanation:

The characteristic that defines a eukaryote is the presence of membrane bound organelles, such as the nucleus, mitochondria, Golgi body, and chloroplasts. Both eukaryotes and prokaryotes have ribosomes, DNA and RNA, and and electron trasnsport chain. Bacteria and plants have cell walls but not all eukaryotes do, so it is not a defining characteristic.

Example Question #4 : Bacteria And Types Of Prokaryotes

Which of the following is not a characteristic of prokaryotes?

Possible Answers:

Presence of mitochondria

Presence of ribosomes

Circular chromosome

Presence of a cell wall

Correct answer:

Presence of mitochondria

Explanation:

Prokaryotes do not have membrane-bound organelles, such as the nucleus or mitochondria. In fact, endosymbiotic theory suggests that mitochondria evolved from symbiotic prokaryotic relationships. Prokaryotes are often associated with all of the other characteristics listed.

Example Question #5 : Bacteria And Types Of Prokaryotes

Which of the following would not help determine whether a cell was eukaryotic or prokaryotic?

Possible Answers:

Circular DNA

Double-stranded DNA

Rough endoplasmic reticulum

Membrane bound organelles

Correct answer:

Double-stranded DNA

Explanation:

Membrane bound organelles are specific only to eukaryotes. Circular DNA is only present in prokaryotes. Rough endoplasmic reticulum is only present in eukaryotes, as all prokaryotic ribosomes are cytoplasmic. Although prokaryotes have circular DNA, both eukaryotes and prokaryotes have double-stranded DNA.

Example Question #6 : Bacteria And Types Of Prokaryotes

Sexually transmitted diseases are a common problem among young people in the United States. One of the more common diseases is caused by the bacterium Neisseria gonorrhoeae, which leads to inflammation and purulent discharge in the male and female reproductive tracts.

The bacterium has a number of systems to evade host defenses. Upon infection, it uses pili to adhere to host epithelium. The bacterium also uses an enzyme, gonococcal sialyltransferase, to transfer a sialyic acid residue to a gonococcal surface lipooligosaccharide (LOS). A depiction of this can be seen in Figure 1. The sialyic acid residue mimics the protective capsule found on other bacterial species.

Once infection is established, Neisseria preferentially infects columnar epithelial cells in the female reproductive tract, and leads to a loss of cilia on these cells. Damage to the reproductive tract can result in pelvic inflammatory disease, which can complicate pregnancies later in the life of the woman.

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What is a key difference between human cells and Neisseria?

Possible Answers:

Human cells have mitochondria, while Neisseria does not

Human cells naturally have sialyc acid on their surface, while Neisseria does not

Human cells produce their own sialyltransferase enzyme, while Neisseria does not

Human cells have ribosomes, while Neisseria does not

Human cells undergo anaerobic metabolism, while Neisseria does not

Correct answer:

Human cells have mitochondria, while Neisseria does not

Explanation:

Prokaryotes like Neisseria do not have mitochondria, while eukaryotic human cells do. In fact, prokaryotes probably gave rise to the first mitochondria via the theory of endosymbiosis.

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