The enzyme helicase unzips the two strands of the double helix. Once unzipped, single stranded binding (SSB) proteins stabilize the newly single strands. The enzyme DNA gyrase ensure the double stranded areas beyond the replication fork do not supercoil onto one another. After stabilization of the replication fork, an enzyme complex known as DNA polymerase III commences the addition of nucleotides to the new strand. Proteins such as the beta clamp and clamp loader assist in keeping DNA polymerase III in its place on the strand of DNA, The enzyme primase adds sequences of RNA primers to the DNA strand to begin replication. DNA Polymerase III cannot begin replication without this primer. DNA ligase reinforces the bonding between the Okazaki fragments and the DNA nucleotides that replace the RNA primer.

DNA synthesis always occurs in the 5' to 3' direction, so one new strand is synthesized continuously towards the replication fork, producing the leading strand. The other strand, known as the lagging strand, forms away from the replication fork in small fragments.

DNA replication occurs both continuously and discontinuously at the same time. Nucleotides can only be added to a new strand of DNA on the 3' end, so the process has to start with the 5' end. As DNA continues to be split apart, the leading strand (growing in the direction towards the replication fork) can continuously add new nucleotides. However, for the lagging strand, the 5' to 3' direction is away from the replication fork, so new nucleotides are added in small chunks called Okazaki fragments as the DNA strand continues to separate.

The enzyme helicase opens the double helix of DNA at points called replication forks.

The unwinding of the double helix of DNA is caused by an enzyme called helicase, which breaks the hydrogen bonds holding the complementary base pairs together, creating two template strands of DNA ready to begin the next step of replication. The place where this enzyme 'unzips' the DNA is called the replication fork.

DNA polymerase is responsible for joining nucleotide subunits to form the new strand of DNA during replication. In contrast, RNA polymerase will join nucleotides to form strands of RNA during transcription. Amylase is found in saliva and catalyzes the breakdown of starch and carbohydrates. Synthases are a class of enzyme that act as catalysts for joining two molecules. Helicase uncoils double-stranded DNA, allowing the formation of the replication fork. 

Replication of chloroplast cells in plants and mitochondrial DNA in advanced cells occurs independently of the cell cycle, follwing instead the process known as D-Loop Replication.

The conversion of DNA into RNA is known as transcription. A DNA template is read to produce a complementary RNA strand.

The conversion of RNA to protein is described by translation, and is completed with the help of mRNA. You cannot transition from DNA straight to protein without these intermediary steps. tRNA is used to carrying amino acids to a growing polypeptide, and rRNA is used to build ribosomes, but these processes are not considered parts of transcription.

DNA stands for "deoxyribonucleic acid." The backbone of DNA is comprised of alternating sugar and phosphate units, in which the sugar is deoxyribose. The backbone of RNA is also comprised of sugar and phosphate units, but uses the sugar ribose.

A DNA molecule has two primary structural domains: the DNA backbone and the DNA bases. Recall that all DNA molecules are made from nucleotides. One nucleotide of a DNA molecule consists of a phosphate group, a pentose (five-carbon) sugar called deoxyribose, and a nitrogenous base (adenine, thymine, guanine, cytosine). Several of these nucleotide monomers are joined together by phosphodiester bonds to create a DNA molecule.

The backbone of a DNA molecule consists of the phosphate groups and the deoxyribose sugars, whereas the base region of the DNA molecule consists of the nitrogenous bases; therefore, the backbone of DNA is made up of phosphate groups and pentose sugars. Adenine is part of the base region of the molecule. DNA does not contain any hexose (six-carbon) sugars.

A DNA molecule is made up of multiple nucleotides that are connected by phosphodiester bonds. A nucleotide consists of a phosphate group, a pentose sugar (deoxyribose in DNA), and a nitrogenous base. The phosphodiester bond occurs between the phosphate group of one nucleotide and the hydroxyl group of the adjacent nucleotide. Recall that the phosphate group is always attached to the 5' carbon on the pentose sugar. There are multiple hydroxyl groups in a pentose sugar, but the hydroxyl group involved in the phosphodiester bond is attached to the 3' carbon; therefore, the phosphodiester bond occurs between a 5' phosphate group and a 3' hydroxyl group. To cut DNA molecules, you need to break these phosphodiester bonds, which is accomplished by the enzyme phosphodiesterase.

Peptide bonds are found in proteins. They are bonds that join adjacent amino acids together and are involved in the formation of a polypeptide (protein) chain. Peptidase proteins are used to break these bonds, effectively cutting proteins, not DNA.

In the nucleus, DNA is always found as a double-stranded molecule. This means that one DNA molecule consists of two DNA strands. Each strand is made up of a DNA backbone (the phosphate groups and the pentose sugars) and the bases.

In a DNA molecule, the two strands are organized in such a way that the DNA backbone of one strand runs in the 5'-to-3' direction, whereas the DNA backbone of the other strand runs in the 3'-to-5' direction; therefore, the two strands are antiparallel to each other.

Recall that 5' and 3' refer to the carbons on the pentose sugar. A phosphate group is found on the 5' carbon of the sugar and a hydroxyl group is found on the 3'carbon of the sugar. This means that the 5' end of each strand is always characterized by the phosphate group, and the 3' end is always characterized by the hydroxyl group; therefore, both strands will have a phosphate group at their 5' end.