Biochemistry : Fundamental Macromolecules and Concepts

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #3 : Lipids

An individual's lipid profile shows low levels of LDL in the blood. What can you conclude about this patient? 

Possible Answers:

There will be increased inflammation in the walls of arteries

There is an increased level of a free 4-membered ring structure in the blood

There is an increased level of prostaglandins in the blood

The patient is at risk for a heart attack

Correct answer:

There is an increased level of a free 4-membered ring structure in the blood

Explanation:

LDL, or low-density lipoprotein, is a lipid transporter that transports lipids in the blood. LDL transports several kinds of lipids such as triglycerides and phospholipids; however, the main lipid transported by LDL is cholesterol. Recall that cholesterol is a 4-membered ring structure with a hydroxyl group attached to one of the rings. A decrease in LDL will decrease the amount of cholesterol carried by LDL which will, subsequently, increase the amount of free cholesterol found in blood; therefore, decreasing LDL will increase the amount of cholesterol found in the blood.

Prostaglandins are lipid molecules derived from arachidonic acid. Recall that arachidonic acid is synthesized from an essential fatty acid called omega-6 fatty acid; therefore, prostaglandins don’t depend on cholesterol and won’t be affected by decreased LDL levels. Heart attacks can often result from atherosclerosis, or thickening and clogging of artery walls due to build up of white blood cells (WBCs). The WBCs typically accumulate in arterial walls due to increased LDL levels; therefore, a decrease in LDL levels will decrease the risk of heart attack. Since lowered LDL levels decrease the amount of WBCs in arterial walls, there will be decreased inflammation. Recall that inflammation is caused by cytokine factors released by WBCs such as macrophages and granulocytes.

Example Question #4 : Lipids

Which of the following is true regarding saturated and unsaturated fatty acids? 

Possible Answers:

Saturated fatty acids can participate in geometric isomerism

A 15-carbon saturated fatty acid has more oxygen atoms than a 15-carbon unsaturated fatty acid

A 15-carbon saturated fatty acid has the same number of hydrogen atoms as a 15-carbon unsaturated fatty acid

A 15-carbon saturated fatty acid has a greater molecular weight than a 15-carbon unsaturated fatty acid

Correct answer:

A 15-carbon saturated fatty acid has a greater molecular weight than a 15-carbon unsaturated fatty acid

Explanation:

Fatty acids are long hydrocarbon chains that contain a carboxylic acid moiety on one end. Saturated fatty acids have no double bonds, whereas unsaturated fatty acids have one or more double bonds. Adding a double bond to a fatty acid will eliminate two hydrogen atoms. Knowing this information, we can deduce that a 15-carbon unsaturated fatty acid with one double bond will have two less hydrogen atoms than a saturated fatty acid of the same length. The molecular formula for the 15-carbon unsaturated fatty acid with one double bond will be , whereas the molecular formula for its saturated counterpart will be . Because of its two additional hydrogen atoms, the saturated fatty acid will have a greater molecular weight.

Geometric isomerism, or cis/trans isomerism, is characterized by the relative positions of functional groups around a double bond. A molecule is ‘cis’ if two identical functional groups are on the same side of the double bond. A molecule is ‘trans’ if the functional groups are on opposite sides. Only unsaturated fatty acids contain double bonds; therefore, only unsaturated fatty acids can participate in geometric isomerism.

Example Question #2 : Lipids

Img 20150717 192426

What is the name of the molecule shown above?

Possible Answers:

Triglyceride

Phosphatidylethanolamine

Phosphatidylserine

Phosphaditic acid

Phosphatidylcholine

Correct answer:

Phosphatidylserine

Explanation:

A triglyceride has three fatty acids; this molecule has two (represented by the R chains). Phosphaditic acid is the simplest of the diacyl-glycerophospholipids; its phosphate group is bonded to only to the glycerol, and nothing else, which is not the case here. Among the three other choices, all of them accurately describe this molecule as a diacylglyceride phospholipid (hence the prefix phosphatidyl-); the phosphate is attached to serine as a head group, not a choline, nor ethanolamine, .

Example Question #1 : Lipids

Which of the following statements concerning lipids are correct?

Possible Answers:

Proteins and carbohydrates embedded in the bilayer impart transverse symmetry to the membrane

An increase in the number of double bonds increases the melting point of a triacylglyceride

Most lipid double bonds are conjugated

Lipids of the bilayer matrix most commonly move by transverse diffusion

Cholesterol is the most common steroid produced in animals

Correct answer:

Cholesterol is the most common steroid produced in animals

Explanation:

Incorrect answers are corrected below:

Most lipid double bonds are conjugated nonconjugated (typically three carbon atoms apart).

Lipids of the bilayer matrix most commonly move by transverse lateral diffusion (on same side of the membrane).

Proteins and carbohydrates embedded in the bilayer impart transverse symmetry asymmetry to the membrane.

An increase in the number of double bonds increases decreases the melting point of a triacylglyceride. 

Example Question #3 : Lipids

In terms of energy storage, __________ store the most energy per unit weight of any molecule in the human body.

Possible Answers:

proteins

carbohydrates

lipids

amino acids

nucleic acids

Correct answer:

lipids

Explanation:

Lipids are capable of storing the most energy per unit weight of any molecule. The complete oxidation of a fatty acid yields , compared to about  for carbohydrates and proteins. 

Example Question #1 : Lipids

Which of the lipids listed below is usually the most abundant phospholipid present in a cell's membrane?

Possible Answers:

Triglycerides

Phosphatidylserine

Phosphatidylinositol

Cholesterol

Phosphatidylcholine

Correct answer:

Phosphatidylcholine

Explanation:

Triglycerides and cholesterol are not phospholipids, so they can be eliminated from the answer choices. Phosphatidylinositol and phosphatidylserine are present in cellular membranes, but they are typically less abundant than phosphatidylcholine, which is the correct answer. 

Example Question #5 : Lipids

Which of the following is not a correct function of cholesterol?

Possible Answers:

A precursor to bile salts

Helps maintain rigidity of blood vessels

All of these are normal functions of cholesterol

A precursor to steroid hormones, such as vitamin D

A component of animal cell membranes

Correct answer:

Helps maintain rigidity of blood vessels

Explanation:

To answer this question, let's go through each of the answer choices to see what cholesterol's functions are.

It turns out that cholesterol is an important component of animal cell membranes. It helps to maintain both the structural integrity of the membrane, as well as its fluidity.

Cholesterol also serves as a precursor for all steroid hormones. In fact, if you look through all the steroid hormones, you'll find that they all have the characteristic 4-ring structure that cholesterol has.

Cholesterol also functions as a precursor for the production of bile salts in the liver. These bile salts are subsequently stored in the gallbladder and, when needed, released into the duodenum of the small intestine to aid in the digestion of lipids.

Cholesterol can deposit into the inner lining of blood vessels, however this is not a normal function of cholesterol. Rather, this is a pathological process that leads to a condition called atherosclerosis. This, in turn, can lead to the hardening of blood vessels, as well as contribute to the formation of blood clots that can impede the flow of blood in that vessel. These clots can also become dislodged and travel throughout the circulatory system, where it can become trapped in other blood vessels. This is a dangerous situation, because it can potentially lead to heart attack or stroke.

Example Question #2 : Lipids

The electric potential gradient of an ion across a plasma membrane __________.

Possible Answers:

increases with temperature

decreases with temperature

is independent of the concentration gradient

is around  in an animal cell at rest

increases as the ion's charge increases

Correct answer:

increases with temperature

Explanation:

For animal cells at rest, the potential difference across plasma membranes is usually somewhere between  and . It is given by the Nernst equation:

Where  and  are constant,  is the ion's charge,  and  are the outside and inside concentrations respectively, and  is the temperature. Therefore, the potential difference decreases as the ion's charge increases, and is not independent of the concentration gradient. It increases -- it does not decrease -- with temperature.

Example Question #11 : Lipids

You have a membrane bilayer composed of phospholipids with saturated fatty acid tails. What can you change to increase the fluidity of this membrane without changing temperature?

Possible Answers:

Remove the phosphate groups

Add membrane proteins

Modify the lipids with oligosaccharides

None of these

Make the fatty acids tails unsaturated by adding cis-double bonds

Correct answer:

Make the fatty acids tails unsaturated by adding cis-double bonds

Explanation:

Lipids with saturated tails are able to pack into very close proximity and form a relatively stable and solid bilayer because the fatty acids tails are straight and do not take up a lot of space. Adding cis-double bonds to these tails puts kinks in the them that make it harder for lipids to pack in close proximity. By unsaturating the tails of lipids in a bilayer membrane in this way, you can make that membrane more fluid.

Example Question #12 : Lipids

What does "amphipathic" mean?

Possible Answers:

Having both hydrophilic and hydrophobic regions

Having both positive and negative charges

Having two stereoisomers

Having both acid and base properties

Correct answer:

Having both hydrophilic and hydrophobic regions

Explanation:

An "amphipathic" molecule is one that has both hydrophilic and hydrophobic regions. A molecule that can act as both an acid and a base is amphoteric. Stereoisomers are molecules with the same molecular formula, but a different arrangement of atoms in space - an example is D-glucose and L-glucose.

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