Biochemistry : Catabolic Pathways and Metabolism

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #1 : Other Lipid Catabolism Concepts

A deficiency of an enzyme in lipid metabolism leads to high levels of triglycerides in the blood. What is the name of the deficient enzyme most likely involved?

Possible Answers:

Acetyl-CoA carboxylase

3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMG-CoA synthase)

Fatty acid synthase

3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase)

Lipoprotein lipase

Correct answer:

Lipoprotein lipase

Explanation:

Lipoprotein lipase is responsible for degrading triglycerides into two fatty acids and a monoacylglycerol molecule. It is attached to the endothelial lumen of the blood vessel. It removes triglycerides from very-low density lipoproteins and chylomicrons in the blood. HMG-CoA reductase and synthase are important enzymes in de novo cholesterol synthesis, but do not cause hypertriglyceridemia (high levels of triglycerides in the blood). Acetyl-CoA carboxylase and fatty acid synthase are part of lipid anabolism, form fatty acids and do not cause hypertriglyceridemia.

Example Question #2 : Other Lipid Catabolism Concepts

How do high-density lipoproteins (HDL) remove cholesterol from the periphery?

I. HDL transfer cholesterol to liver cells through the scavenger receptor SR-B1

II. HDL transfer cholesterol to intermediate-density lipoproteins using the cholesterol transfer protein

III. HDL removes cholesterol accumulating in blood vessels

IV. HDL is taken up by macrophages in atherosclerotic plaques

Possible Answers:

I, II, and III

II and IV

I and II

I and IV

II and III

Correct answer:

I, II, and III

Explanation:

Cholesterol and lipids are carried in the blood by lipoproteins. HDL are lipoproteins that remove cholesterol accumulated in blood vessels and take it to the liver for excretion in the bile or further processing by steroidogenic tissues. HDL delivers cholesterol to liver cells through the scavenger receptor SR-B1. HDL can also transfer cholesterol to intermediate-density lipoproteins (IDL) using the cholesterol transfer protein. Incorporation of LDL (low-density lipoproteins), not HDL by macrophages leads to formation of fatty streaks in atherosclerotic plaques. This does not remove cholesterol from periphery, but rather contributes to it. 

Example Question #41 : Lipid Catabolism

What happens when the acetyl-CoA produced from beta-oxidation can not enter into the Krebs Cycle due to a lack of oxaloacetate from starvation?

Possible Answers:

The acetyl-CoA molecules combine to form acetoacetyl-CoA which is used as energy

The acetyl-CoA molecules form ketone bodies

The acetyl-CoA molecules combine to form 3-hydroxy-3-methyl-glutaryl-CoA which is used as energy

The acetyl-CoA molecules go through the gluconeogenesis pathway to form more glucose

The acetyl-CoA molecules combine with another Krebs cycle intermediate to continue with oxidative respiration

Correct answer:

The acetyl-CoA molecules form ketone bodies

Explanation:

When oxaloacetate is low in the body as a result of starvation, acetyl-CoA can no longer combine with it to continue through the Krebs cycle. Oxaloacetate can go through gluconeogenesis to form more glucose, however acetyl-CoA does not. Instead, the acetyl-CoA molecules go through a series of steps (acetoacetyl-CoA and 3-hydroxy-3-methyl-glutaryl-CoA are intermediate molecules in these steps) to form ketone bodies.

Example Question #42 : Lipid Catabolism

Which of the following statements is true about the role of apolipoprotein B (ApoB)100 in lipid metabolism?

I. ApoB 100 is synthesized by the liver.

II. ApoB 100 is a component of very low density, intermediate density and low density lipoproteins circulating in the blood.

III. ApoB 100 is a ligand for the LDL (low density lipoprotein) receptor in cells requiring intake of cholesterol.

IV. ApoB 100 is encoded by the same gene that produces ApoB 48.

Possible Answers:

I and II

I, II, III, and IV

I and IV

I, II, and III

II, III, and IV

Correct answer:

I, II, III, and IV

Explanation:

Apoliporoteins carry lipids in the blood as lipids are insoluble. ApoB100 is a protein found on different types of lipoproteins circulating in the body. ApoB 48 is another apolipoprotein that is present on chylomicrons. Both ApoB 100 and ApoB 48 are encoded by the ApoB gene, but ApoB 48 is shorter than ApoB 100 and is produced in the intestine.

Example Question #1 : Carbohydrate Metabolism

In photosynthesis, what is the purpose of absorbing light into chloroplasts?

Possible Answers:

The energy from the absorbed light generates NADPH from the electrons of water molecules, ultimately leading to the creation of carbon dioxide

The energy from the absorbed light moves the electrons from NADPH to water molecules, ultimately setting up a proton gradient

The energy from the absorbed light generates NADPH from the electrons of water molecules, ultimately setting up a proton gradient

The energy from the absorbed light causes the generation of NADH from the electron of water molecules, ultimately setting up a proton gradient

The energy from the absorbed light causes all of the electrons in the chloroplast to jump to higher energy levels

Correct answer:

The energy from the absorbed light generates NADPH from the electrons of water molecules, ultimately setting up a proton gradient

Explanation:

In photosynthesis, light is absorbed in order to move electrons from water molecules to NADPH.  The reduction of  to NADPH is accompanied by the movement of protons across a membrane which sets up a gradient similar to that of oxidative phosphorylation.  The protons eventually run through ATP synthase and ATP is formed.

Example Question #76 : Catabolic Pathways And Metabolism

Photosynthesis consists of a light phase and a dark phase. The light phase precedes the dark phase and supplies it with __________.

Possible Answers:

Correct answer:

Explanation:

During photosynthesis, the light phase is responsible for creating  which the dark phase then consumes as a part of its process. In addition to  is used in the dark phase as a high energy substrate to work properly.

Example Question #1 : Carbohydrate Metabolism

In photosynthesis, if photosystem II absorbs 12 photons, how many molecules of  would be produced?

Possible Answers:

Correct answer:

Explanation:

Absorbing four photons by photosystem II creates one oxygen molecule, so absorbing 12 would produce 3 molecules of .

Example Question #78 : Catabolic Pathways And Metabolism

During photosynthesis there is both a light phase and a dark phase. If the light phase were to continue unabated, but the dark phase came to a halt, which of the following is most likely to occur?

Possible Answers:

Increased sensitivity of the chloroplast to incoming light

A proportional decrease in  

An increase in the production of carbohydrates 

Decreased sensitivity of the chloroplast to incoming light

Decrease in the level of 

Correct answer:

Decrease in the level of 

Explanation:

During photosynthesis, the dark phase follows the light phase. The light phase produces  which, along with , is fed into the dark phase where it is consumed (becomes  and ). If the light phase continues working, but the dark phase does not, the  and  created during the light phase will not be consumed. Thus, there will be a decrease in the level of  and  (the correct answer). The relative proportion of  will actually increase.

Carbohydrate production is a result of the dark phase working properly, so their levels would decrease in this instance. The sensitivity of the chloroplast to light would not change.

Example Question #1 : Calvin Cycle Carbohydrate Intermediates

What happens during stage 1 of the Calvin cycle?

Possible Answers:

Fructose-6-phosphate becomes ribulose 5-phosphate

Carbon dioxide and ribulose 1,5-bisphosphate react to form 3-phosphoglycerate

ATP reacts with ribulose 5-phosphate to become ribulose 1,5-bisphosphate

2 ATP molecules react with 3-phosphoglycerate to form 1,3-bisphosphoglycerate

NADPH and 1,3 bisphosphoglycerate react to form glyceraldehyde-3-phosphate

Correct answer:

Carbon dioxide and ribulose 1,5-bisphosphate react to form 3-phosphoglycerate

Explanation:

All of the answer choices are steps in the Calvin cycle, but the only one that describes the first stage - fixation - is  and ribulose 1,5-bisphosphate reacting to form 3-phosphoglycerate.

Example Question #1 : Photosynthesis

What are the three stages of the Calvin cycle?

Possible Answers:

Carboxylation, reduction, and regeneration

Fixation, reduction, and regeneration

Fixation, carboxylation, and regeneration 

Reduction, oxidation, and regeneration

Fixation, oxidation, and regeneration

Correct answer:

Fixation, reduction, and regeneration

Explanation:

The stages of the Calvin cycle in the order that they occur are fixation, reduction, and then regeneration.  While carboxylation does occur as a part of the first stage, it is not what defines that stage.

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