Biochemistry : Electron Transport and Oxidative Phosphorylation

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #161 : Catabolic Pathways And Metabolism

Which of the following is true regarding the aerobic combustion of glucose to yield water and carbon dioxide?

Possible Answers:

All of these

It requires oxygen

It has a negative Gibbs free energy

It is thermodynamically favorable

Correct answer:

All of these

Explanation:

The combustion of glucose to yield carbon dioxide and water refers to aerobic metabolism (oxidative phosphorylation). This process releases energy, so Gibbs free energy is negative. A negative Gibbs free energy indicates that the products are at a lower energy than the reactants, meaning that the reaction is thermodynamically favorable (spontaneous). Lastly, aerobic metabolism is called so because it requires oxygen. Thus, all of the answers are correct.

Example Question #2 : Electron Transport Chain Energetics

What is the action of the enzyme complexes involved in the electron transport chain?

Possible Answers:

High energy electrons pass through the complexes in order to reduce  and  to  and 

High energy electrons pass through the complexes in order to ultimately turn water molecules into oxygen

High energy electrons pass through them resulting in the pumping of hydrogens across the mitochondrial inner membrane

The complexes directly create ATP as high energy electrons pass through them

High energy electrons pass through the complexes in order to create glucose molecules

Correct answer:

High energy electrons pass through them resulting in the pumping of hydrogens across the mitochondrial inner membrane

Explanation:

The complexes that function as a part of the electron transport chain accept electrons from  and .  When they accept the high energy electrons, they also pump hydrogens across the mitochondrial inner membrane to ultimately be used at the ATP synthase.  Creation of ATP is the end goal, but it is not made directly by the electron transport chain complexes.

Example Question #3 : Electron Transport Chain Energetics

Why is oxygen required for the electron transport chain to function properly?

Possible Answers:

Oxygen is converted to water by the addition of hydrogen atoms which can then accept electrons from the electron transport chain

Oxygen allows the electrons from  and  to enter into the electron transport chain

Oxygen accepts electrons that have run through the electron transport chain 

Oxygen acts as a catalyst for the pumping of hydrogens through the protein complexes in the electron transport chain

Oxygen reattaches hydrogen and electrons to  and  after the electron transport chain is finished 

Correct answer:

Oxygen accepts electrons that have run through the electron transport chain 

Explanation:

Oxygen is required for the electron transport chain to function because it is the final electron acceptor for oxidative respiration. Once the high energy electron carriers,  and , have delivered electrons to the chain, the electrons run through the protein complexes. When they finish moving through all of the complexes, something must be available for them to attach to. Oxygen is the molecule responsible for this. It accepts the electrons and in turn is converted into .

Example Question #1 : Atp Synthase

Which of the following is false about the structure and function of ATP synthase?

Possible Answers:

It is a membrane-bound protein.

The rotor has 10 to 14 subunits.

There are six subunits in its ring.

It synthesizes over 100 ATP molecules per second.

At least 10 protons pass through ATP synthase in order to make a molecule of ATP.

Correct answer:

At least 10 protons pass through ATP synthase in order to make a molecule of ATP.

Explanation:

ATP synthase can indeed produce more than 100 ATP molecules per second, and in the process, it only requires a few -- three or four -- protons, per ATP. These protons pass down a gradient through the membrane. Hence, the protein is membrane-bound. The protons cause the rotor of 10-14 subunits to spin. The protein's head itself has six subunits, three of which have ADP binding and phosphate binding sites.

Example Question #21 : Electron Transport And Oxidative Phosphorylation

What is the basic mechanism by which ATP synthase generates ATP in the mitochondria?

Possible Answers:

The formation of ATP is energetically favorable and does not require a synthase to be formed

ATP synthase uses an electron gradient set up by oxidative phosphorylation to drive ATP synthesis

The high energy electrons from oxidative phosphorylation react with the separate components of ATP and drive them together by using ATP synthase

ATP synthase uses energy from G-protein complexes to phosphorylate ADP to ATP

The proton gradient set up by the electron transport chain causes mechanical rotation of ATP synthase, and this energy is used to form ATP

Correct answer:

The proton gradient set up by the electron transport chain causes mechanical rotation of ATP synthase, and this energy is used to form ATP

Explanation:

The electron transport chain moves high energy electrons through its complexes in order to create a proton gradient across the mitochondrial inner membrane.  The ATP synthase then uses this gradient to pass hydrogen atoms through it.  Because this is a favorable movement, it can be coupled to unfavorable processes such as conversion of ADP to ATP.  

Example Question #23 : Electron Transport And Oxidative Phosphorylation

ATP synthase catalyzes which of the following reactions?

Possible Answers:

Correct answer:

Explanation:

ATP synthase catalyzes the reaction that shows ADP and the phosphate group forming ATP.  The hydrogen in the reactant side is the one involved in the proton gradient, and water is a byproduct of the reaction.

Example Question #24 : Electron Transport And Oxidative Phosphorylation

Which of the following part(s) of ATP synthase is involved in catalysis?

Possible Answers:

Beta subunit

Gamma subunit 

Alpha subunit

Alpha, beta, and gamma subunits

Alpha and beta subunits

Correct answer:

Beta subunit

Explanation:

ATP synthase is located in the inner mitochondrial membrane. It has an F0 portion within the membrane and an F1 portion in the matrix. The F1 portion has a hexameric ring structure and is responsible for the creation of ATP from mechanical energy. The alpha, beta, and gamma subunits are all parts of the F1 portion of ATP synthase, however it is only the alpha and beta subunits that form the ring. Further, the beta subunit is the part of the ring that is considered to be catalytic.

Example Question #21 : Electron Transport And Oxidative Phosphorylation

Below are standard reduction potentials of components in carbohydrate metabolism 

 

 

What is the free energy change for this reaction?

Possible Answers:

Correct answer:

Explanation:

First, let's consider the half reactions involved to determine 

This overall reaction involves the donation of 2 electrons, so

 is defined as . The reaction we drew earlier is shown below:

We can see that  was oxidized and  was reduced. Find .

 is Faraday's constant, and is defined as: 

Solve for 

 

Example Question #22 : Electron Transport And Oxidative Phosphorylation

Which of the following processes involved in cellular respiration has a positive Gibbs Free energy?

Possible Answers:

The pumping of hydrogens from the mitochondrial matrix to the intermembrane space

The final step in glycolysis

The combination of oxaloacetate and acetyl-CoA to form citrate in the Kreb's cycle

The movement of the electrons through the electron transport chain

The movement of hydrogens through ATP synthase

Correct answer:

The pumping of hydrogens from the mitochondrial matrix to the intermembrane space

Explanation:

A positive Gibbs free energy implies that the process in question should be unfavorable under normal conditions.  The only process listed that is unfavorable and requires an input of energy is the pumping of hydrogen ions into the intermembrane space.  This occurs during the electron transport chain.

Example Question #1 : Oxidative Phosphorylation Energetics

In what phase of cellular respiration is not ATP produced?

Possible Answers:

Every phase of cellular respiration produces some ATP

Glycolysis

Pyruvate dehydrogenase complex

Krebs cycle

Electron transport chain

Correct answer:

Pyruvate dehydrogenase complex

Explanation:

The phases of cellular respiration are glycolysis, pyruvate dehydrogenase complex, Krebs cycle, electron transport chain. Glycolysis produces a net total of 2 ATP, the Krebs cycle produces 1 GTP that is converted to ATP in another process, and the electron transport chain is where almost all of the ATP made in cellular respiration is formed. However, during the pyruvate dehydrogenase complex phase of cellular respiration, pyruvate is converted to acetyl-CoA as a preparation for the Krebs cycle, but no ATP is created.

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