Biochemistry : Biochemistry

Study concepts, example questions & explanations for Biochemistry

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Example Questions

Example Question #1 : Light Reactions

Photosynthesis consists of a light phase and a dark phase. The light phase precedes the dark phase and supplies it with __________.

Possible Answers:

Correct answer:

Explanation:

During photosynthesis, the light phase is responsible for creating  which the dark phase then consumes as a part of its process. In addition to  is used in the dark phase as a high energy substrate to work properly.

Example Question #2 : Carbohydrate Metabolism

In photosynthesis, if photosystem II absorbs 12 photons, how many molecules of  would be produced?

Possible Answers:

Correct answer:

Explanation:

Absorbing four photons by photosystem II creates one oxygen molecule, so absorbing 12 would produce 3 molecules of .

Example Question #2 : Light Reactions

During photosynthesis there is both a light phase and a dark phase. If the light phase were to continue unabated, but the dark phase came to a halt, which of the following is most likely to occur?

Possible Answers:

A proportional decrease in  

Decrease in the level of 

An increase in the production of carbohydrates 

Decreased sensitivity of the chloroplast to incoming light

Increased sensitivity of the chloroplast to incoming light

Correct answer:

Decrease in the level of 

Explanation:

During photosynthesis, the dark phase follows the light phase. The light phase produces  which, along with , is fed into the dark phase where it is consumed (becomes  and ). If the light phase continues working, but the dark phase does not, the  and  created during the light phase will not be consumed. Thus, there will be a decrease in the level of  and  (the correct answer). The relative proportion of  will actually increase.

Carbohydrate production is a result of the dark phase working properly, so their levels would decrease in this instance. The sensitivity of the chloroplast to light would not change.

Example Question #1 : Calvin Cycle

What happens during stage 1 of the Calvin cycle?

Possible Answers:

ATP reacts with ribulose 5-phosphate to become ribulose 1,5-bisphosphate

NADPH and 1,3 bisphosphoglycerate react to form glyceraldehyde-3-phosphate

Carbon dioxide and ribulose 1,5-bisphosphate react to form 3-phosphoglycerate

Fructose-6-phosphate becomes ribulose 5-phosphate

2 ATP molecules react with 3-phosphoglycerate to form 1,3-bisphosphoglycerate

Correct answer:

Carbon dioxide and ribulose 1,5-bisphosphate react to form 3-phosphoglycerate

Explanation:

All of the answer choices are steps in the Calvin cycle, but the only one that describes the first stage - fixation - is  and ribulose 1,5-bisphosphate reacting to form 3-phosphoglycerate.

Example Question #1 : Carbohydrate Metabolism

What are the three stages of the Calvin cycle?

Possible Answers:

Fixation, carboxylation, and regeneration 

Reduction, oxidation, and regeneration

Fixation, oxidation, and regeneration

Carboxylation, reduction, and regeneration

Fixation, reduction, and regeneration

Correct answer:

Fixation, reduction, and regeneration

Explanation:

The stages of the Calvin cycle in the order that they occur are fixation, reduction, and then regeneration.  While carboxylation does occur as a part of the first stage, it is not what defines that stage.

Example Question #2 : Carbohydrate Metabolism

Which of the following is not true of the Calvin cycle?

Possible Answers:

NADPH and ATP are required to initiate the Calvin cycle

All of these are true of the Calvin cycle

The Calvin cycle does not need light to function

The Calvin cycle occurs in the stroma of a chloroplast

The first step of the Calvin cycle is carbon fixation

Correct answer:

The Calvin cycle does not need light to function

Explanation:

Despite the Calvin cycle's other name (light independent reactions), light is indirectly required for this process to function. All other choices are true of the Calvin cycle.

Example Question #801 : Biochemistry

Which of these enzymes catalyzes the first reaction in glycolysis?

Possible Answers:

Triose phosphate isomerase

Pyruvate kinase

Aldolase

Hexokinase

Correct answer:

Hexokinase

Explanation:

The first step in glycolysis is the conversion of glucose to glucose-6-phosphate through the consumption on one ATP molecule. Glucose is reacted upon by the enzyme hexokinase to carry out this step. Kinases are a group of enzymes that add phosphate groups by removing them from an ATP. All of these other enzymes catalyze subsequent reactions in glycolysis.

Example Question #1 : Glycolysis Enzymes

Dihydroxyacetone is converted to glyceraldehyde-3-phosphate by what category of enzyme?

Possible Answers:

Dehydrogenase

Isomerase

Enolase

Kinase

Correct answer:

Isomerase

Explanation:

Dihydroxyacetone phosphate (DHAP) is converted to glyceradehyde-3-phosphate (G3P) by the enzyme triose phosphate isomerase. As the name suggests, this enzyme catalyzes the isomerization of a three-carbon sugar into another three-carbon sugar. Since the molecular formulas of DHAP and G3P are the same, we know that they are isomers of each other.

The balance between DHAP and G3P is extremely important in regulating overall cell metabolism. DHAP is a precursor to triglycerides, and is used in their synthesis, while G3P is an intermediate in glycolysis, an ATP-producing process. In order to favor the conversion of DHAP into G3P, and not the opposite, the cell must keep G3P levels low (Le Chatelier's Principle). Consider the following equilibrium: . This should make sense: if there is lots of ATP around in the cell, there is no need for glycolysis to proceed. Thus the equilibrium will be pushed to the left, increasing the concentration of DHAP in the cell. In humans, DHAP is converted into triglycerides, which get stored as fat. One way to shift this equilibrium to the right is to "create" an ATP need. This can be done by exercising. Exercise utilizes ATP and will thus pull the equilibrium to the right, removing DHAP (which was destined to be converted into fat) and facilitates its conversion into G3P to proceed with cellular respiration. 

Example Question #801 : Biochemistry

The enzyme pyruvate kinase is responsible for catalyzing the conversion of phosphoenolpyruvate into __________.

Possible Answers:

glucose

glucose-6-phosphate

dihydroxyacetone

pyruvate

Correct answer:

pyruvate

Explanation:

The tenth and final reaction of glycolysis involves the conversion of phosphoenolpyruvate (PEP) into pyruvate. This step is catalyzed by the enzyme pyruvate kinase. This kinase is going to remove a phosphate group from PEP and put it on ADP to yield ATP. Pyruvate, a three-carbon molecule, is the end product of glycolysis. It can be sent to the pyruvate dehydrogenase complex to be turned into acetyl-CoA, which enters the Krebs cycle. Alternatively, it can be reduced into lactate and/or ethanol (depending on the organism) to regenerate  for glycolysis via anaerobic respiration.

Example Question #1 : Glycolysis Enzymes

The enzyme enolase catalyzes the ninth reaction of glycolysis. What is the product of this reaction?

Possible Answers:

Phosphoenolpyruvate (PEP)

glyceraldehyde-3-phosphate (G3P)

Dihydroxyacetone phosphate

Pyruvate

Correct answer:

Phosphoenolpyruvate (PEP)

Explanation:

The ninth reaction involves the conversion of 2-phosphoglycerate into phosphoenolpyruvate. The enzyme enolase, which produces a double bond by removing the hydroxyl group on 2-phosphoglycerate catalyzes this reaction. Note that the resulting molecule is an enol (double bond -ene, and alcohol - ol).

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