AP Biology : Understanding Ribosomes, tRNA, and Anti-codons

Study concepts, example questions & explanations for AP Biology

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Example Questions

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Example Question #31 : Translation

Which of the following choices will most likely be found in the aminoacyl site of the large ribosomal unit?

Possible Answers:

The tRNA molecule with the next amino acid to be added to the polypeptide chain

The amino-acid free tRNA

The growing polypeptide chain

Initiation factors

Correct answer:

The tRNA molecule with the next amino acid to be added to the polypeptide chain

Explanation:

The large ribosomal subunit has three sites that interact with tRNA molecules—the peptidyl “P” site, the aminoacyl “A” site, and the exit “E” site. The P-site holds the tRNA corresponding to the most recently added amino acid, which is attached to the growing polypeptide chain by a peptide bond. The A-site holds the tRNA with the next amino acid to be added to the chain. Finally, the E-site holds the free tRNA without an amino acid that was previously in the P-site.

Example Question #12 : Understanding Ribosomes, T Rna, And Anti Codons

If the codon in the mRNA strand is 5' CAG, what is the anticodon strand sequence?

Possible Answers:

5' GUC

3' GUC

3' GTC

3' CUG

5' GTC

Correct answer:

3' GUC

Explanation:

The mRNA strand is synthesized 5' to 3' and contains the codons. tRNA contains the anticodons needed for the corresponding amino acid, and is paired to the codon 3' to 5'.

Example Question #31 : Translation

The mRNA codon for methionine is 5' AUG 3'. What is the corresponding anticodon found on the methionine tRNA?

Possible Answers:

3' AUG 5'

5' ATG 3'

5' UAG 3'

3' UAC 5'

3' TAC 5'

Correct answer:

3' UAC 5'

Explanation:

The anticodon of any codon will be the RNA nucleotides that complement the codon sequence. In RNA, adenine (A) complements uracil (U) while cytosine (C) complements guanine (G). Hence, for the codon 5' AUG 3', the complements will be 3' UAC 5'. Note that many of the incorrect answers contain thymine (T), a nucleotide not found in RNA.

Example Question #32 : Translation

Which of the following amino acids is responsible for the initiation of the translation of a polypeptide?

Possible Answers:

Asparagine

Methionine

Alanine

Leucine

Valine

Correct answer:

Methionine

Explanation:

The start codon for any strand of RNA begins with the codon that codes for the amino acid methionine. This is the first amino acid in a polypeptide chain. The abbreviation for methionine is: Met.

Example Question #11 : Understanding Ribosomes, T Rna, And Anti Codons

Proteins that will be secreted outside of the plasma membrane of the cell are translated from ribosomes found __________.

Possible Answers:

on the plasma membrane

in the cytosol

in the nucleus

on the rough endoplasmic reticulum

on the smooth endoplasmic reticulum

Correct answer:

on the rough endoplasmic reticulum

Explanation:

When translated from a ribosome on the rough endoplasmic reticulum (RER), the protein is moved through the RER until it is released in a lipid vesicle that can transport the protein to the plasma membrane, where the lipid vesicle fuses with the lipid membrane and the protein is secreted outside the cell. Smooth endoplasmic reticulum does not have ribosomes on it and is used for the production and transport of lipids and in detoxification. No ribosomes are found in the nucleus or directly on the plasma membrane. Ribosomes in the cytosol translate proteins that stay inside the cell.

Example Question #113 : Dna, Rna, And Proteins

In messenger RNA, each codon specifies a particular __________.

Possible Answers:

amino acid

nucleotide

pyrimidine

purine

Correct answer:

amino acid

Explanation:

In messenger RNA, each codon is three nucleotides that codes for a particular amino acid during translation. Purines and pyrimidines are types of nucleotides on DNA and RNA. The genetic code is redundant, but each codon only codes for one amino acid.

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