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Example Questions
Example Question #11 : Properties Of Nucleic Acids
Which nucleic acid carries information coding for an amino acid sequence out from the nucleus towards a ribosome in the cytoplasm?
rRNA
xDNA
mRNA
mDNA
tRNA
mRNA
All of these are actual nucleic acids, except xDNA, which does not exist. Therefore, we can eliminate this answer right away. mtDNA, or mitochondrial DNA, is a small portion of a person's DNA that is housed within the mitochondria. It is significant in that it is inherited solely from the mother and is thought to have evolved separately from "normal" DNA; however, it isn't involved with amino acids at all, so it too can be eliminated as a choice.
RNA (ribonucleic acid) is heavily involved in protein synthesis (the creation of new proteins, which are just long chains of amino acids). The first part of the process is transcription, in which the double-stranded DNA (deoxyribonucleic acid) is "unzipped" by the enzyme helicase. Another enzyme, RNA polymerase, "reads" this single strand and produces a chain of nucleotides exactly opposite to the DNA's nucleotides, and this chain, in its mature form, is called mRNA (messenger RNA). Living up to its name, mRNA travels outside the nucleus, where DNA is housed, and out into the rest of the cell, to the ribosome.
At this point, translation begins. Every trio of nucleotides on the mRNA, called a codon, represents a specific amino acid. Another type of RNA, tRNA (transfer RNA) can "translate" the codon into the appropriate amino acid, since it carries a three-nucleotide-long anticodon on one segment, which can form a bond with the corresponding codon on the mRNA, and an amino acid on the other segment. In this way tRNA brings amino acids to the growing protein chain. Another involved nucleic acid is rRNA (ribosomal RNA) which makes up a large part of the ribosome itself and is responsible for helping to properly attach each amino acid that the tRNA brings to the amino acid sequence being built.
Based on the question, which asks you to identify the nucleic acid that helps carry the protein "message" from the nucleus to the ribosome, the correct answer is mRNA.
Example Question #11 : Properties Of Nucleic Acids
Which of the following codons serves as the initiation site when a mRNA transcription begins to be translated?
CUG
UAG
UAA
AUG
UGA
AUG
In DNA transcription and translation, the start codon is the first codon, among those transcribed into the mRNA sequence, that is translated during protein synthesis. In humans, this is always AUG, which codes for the amino acid methionine.
Of the other answer choices, three function as a stop codon: UAA, UGA, and UAG. The other choice, CUG, codes for the amino acid leucine.
A mnemonic for remembering that AUG is the start codon is to make the connection that the school year typically starts in August.
Example Question #12 : Properties Of Nucleic Acids
After sequencing the genome of a newly discovered virus (virus A), you find that it is composed of 18% Adenine bases, consists of one double stranded loop, and contains deoxyribose sugar.
What percentage of the genome is composed of Guanine bases?
64%
32%
36%
18%
32%
According to Chargoff's rules, we know that the ratios of Adenine:Thymine and Guanine: Cytosine will be approximately 1:1. Once you know that the percentage of Adenine is 18%, you can multiply by two to find the total percentage of Thymine and Adenine (36%), then, you can subtract that number from 100% to find the percentage of Cytosine and Guanine - 64% (because the total percentage has to equal 100%), finally you can divide it in half to get the percentage of Guanine bases (32%).
Example Question #13 : Properties Of Nucleic Acids
After sequencing the genome of a newly discovered virus (virus A), you find that it is composed of 18% Adenine bases, consists of one double stranded loop, and contains deoxyribose sugar.
What percentage of the genome is composed of Uracil bases?
18%
36%
0%
64%
0%
Because of the presence of Deoxyribose sugar, you know that the genome is composed of DNA. Only RNA contains Uracil bases, so the answer is 0%.
Example Question #14 : Properties Of Nucleic Acids
What is the type of sugar found in all nucleic acids?
pentose
deoxyribose
hexose
ribose
pentose
Every nucleic acid is made up of 3 parts — a pentose (5 membered ring) sugar, a phosphate group and a nitrogenous base.
A hexose sugar has a six membered ring. Ribose and deoxyribose are types of sugars found in nucleic acids, but are only found in RNA and DNA respectively.
Example Question #15 : Properties Of Nucleic Acids
Which is not a difference between RNA and DNA?
RNA contains Uracil and DNA contains Thymine
DNA is often maintained in its B-form and RNA is usually in it's A-form
RNA has a hydroxyl group in the 2' position in its pentose sugar and DNA does not
DNA is always single stranded and RNA is always double stranded
DNA is always single stranded and RNA is always double stranded
While DNA is usually found double stranded and RNA is often found single stranded, RNA can also be found in double stranded forms, notably in viruses.
Example Question #16 : Properties Of Nucleic Acids
Which of the following is a purine?
Adenine
Thymine
Cytocine
Uracil
Adenine
Adenine and Guanine are purines and consist of two connected nitrogenous rings. Cytosine, Uracil, And Thymine are pyrimidines and consist of one nitrogenous rings.
One can remember this by remembering that stones had to be CUT in order to build the PYRAMIDs.
Example Question #18 : Properties Of Nucleic Acids
What property of nucleic acids allows for them to be detected by UV absorbance?
The pentose sugars contain pi bonds.
The nitrogenous bases are aromatic.
The phosphate group is high energy.
The phosphodiester bond contains pi bonds
The nitrogenous bases are aromatic.
The aromatic nitrogenous bases absorb UV radiation, which allow nucleic acids to be detected by UV absorbance.
Example Question #17 : Properties Of Nucleic Acids
What of the following is true about nucleotides and nucleosides?
Nucleosides only have a phosphate group attached to the sugar.
Nucleotides only have a phosphate group attached to the sugar.
There is no difference.
Nucleotides have a phosphate group and a nitrogenous base attached to the sugar.
Nucleotides have a phosphate group and a nitrogenous base attached to the sugar.
Nucleosides have only a nitrogenous base attached to the sugar. Nucleotides have a phosphate group and a nitrogenous base attached to the sugar. An easy way to remember this is that nucleoSides have a single group attached to the sugar, and nucleoTides have two groups attached to the sugar.
Example Question #18 : Properties Of Nucleic Acids
After sequencing the genome of a newly discovered virus (virus A), you find that it is composed of 18% Adenine bases, consists of one double stranded loop, and contains deoxyribose sugar.
Later on, your lab finds a new virus (Virus B), analyzes its genome, and finds that the new virus' genome is the same length but composed of 25% Adenine bases. Which viral genome will have the higher melting temperature?
both have the same melting temperature
Virus B's genome
There is not enough information to answer the question.
Virus A's genome
Virus A's genome
Using Chargoff's rules, one can calculate that Virus A has 64% C-G pairs in its genome and Virus B has 50% C-G base pairs in its genome. Since C-G pairs have an additional hydrogen bond, they are more stable than A-G pairs, so more energy will be required to separate the strands and "melt" the DNA. Since the two genomes are the same length, the stacking effects will be the same, so the deciding factor will be base pair composition, and Virus A's genome will have a higher melting temperature.
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