AP Biology : Understand steps of transcription

Study concepts, example questions & explanations for AP Biology

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Example Questions

Example Question #11 : Understand Steps Of Transcription

Which of the following is the central dogma of biology?

Possible Answers:

Correct answer:

Explanation:

The genetic information in a cell is stored in its DNA. Through transcription, the cell is able to create RNA from DNA. This RNA is called mRNA. The mRNA is then able to create protein through translation. Thus, the flow of information is from DNA to RNA to protein. 

Example Question #11 : Central Dogma

What type of chromatin would be found in telomeres and centromeres?

Possible Answers:

Both heterochromatin and euchromatin

Heterochromatin

Either heterochromatin or euchromatin, depending on the cell type

Euchromatin

Neither heterochromatin nor euchromatin

Correct answer:

Heterochromatin

Explanation:

Telomeres and centromeres are composed of heterochromatin. In contrast to euchromatin, heterochromatin's genes are generally in an inactive state. This is because the genetic material is highly condensed. Often, heterochromatin is thought of as "junk DNA". Since telomeres are slowly being degraded during DNA replication, the cell does not want to have active or important genes in this area. Same goes for centromeres, where there is the possibility of chromatids not separating evenly across the centromere in anaphase. 

Example Question #11 : Central Dogma

If a DNA template (the strand actively being transcribed) begins with AAGGCTCGGAA, what will the nascent RNA template begin with?

Possible Answers:

AAGGCTCGGAA

TTCCGAGCCTT

UUCCGAGCCUU

AUGUGUTTCUG

CCTTAGATTCC

Correct answer:

UUCCGAGCCUU

Explanation:

Simply create a complementary strand of RNA with matching base pairs, but substitute uracil (U) for thymine (T), since RNA does not use thymine base pairs.

The answer choice starting with AUG might seem appropriate since it is the start codon, however, realize that the rest of the base pairs do not match up to the DNA template. Further, RNA templates do not begin right at the start codon; there are promotoer and enhancer regions of DNA that are transcribed well before the first exon is transcribed with its AUG start codon.

Example Question #12 : Central Dogma

Eukaryotic DNA is packed in a chromatin structure, making it hard for DNA to be transcribed. What proteins are associated with the packing of DNA? 

Possible Answers:

Actin

Polymerases

Histones

Myosin

Collagen

Correct answer:

Histones

Explanation:

Histones are associated with DNA packed in chromatin. Acetylation of these histones allows for DNA transcription. Other proteins mentioned play no part in DNA chromatin structure.

Polymerases are active during DNA repair and transcription. Collagen is a fibrous protein associated with the extracellular matrix. Actin and myosin are myofilaments active in muscle contraction.

Example Question #11 : Understand Steps Of Transcription

In transcription, the promoter is __________.

Possible Answers:

a protein that increases the transcription levels for a given gene

a sequence of DNA that designates the starting point for RNA polymerase

an RNA sequence that signals the start of translation

a protein that guides RNA polymerase to the starting point

Correct answer:

a sequence of DNA that designates the starting point for RNA polymerase

Explanation:

Transcription requires a DNA sequence that signals the RNA polymerase where to begin transcribing a given gene. The promoter is the DNA sequence that allows the RNA polymerase to bind to the right spot on the DNA and begin transcription. This prevents transcription of partial proteins or protein fragments that would be non-functional and possibly harmful to the cell.

Example Question #12 : Understand Steps Of Transcription

Where does transcription take place in eukaryotic cells?

Possible Answers:

The cytoplasm

The rough endoplasmic reticulum

The nucleus

Ribosomes

Correct answer:

The nucleus

Explanation:

Transcription is the process of synthesizing RNA from a DNA template. In eukaryotic cells, chromosomal DNA is contained within the nucleus. Transcription requires access to this DNA, and therefore must occur in the nucleus. Resulting RNA molecules are then shuttled out of the nucleus to be used in other processes.

Example Question #13 : Understand Steps Of Transcription

Where does transcription occur in a cell?

Possible Answers:

Nucleolus

Rough endoplasmic reticulum

Mitochondria

Lysosome

Nucleus

Correct answer:

Nucleus

Explanation:

The DNA gets transcribed into RNA inside the nucleus. This is where DNA is housed; DNA never leaves the nucleus (except during mitosis, during which the nuclear envelope will briefly disappear so that two cells can be formed). After DNA gets transcribed into RNA, the RNA is modified and eventually transported out of the nucleus as mRNA, which is now ready for translation.

Translation occurs on ribosomes, which can either be bound to the rough endoplasmic reticulum or free-floating in the cytoplasm. The nucleolus is a structure within the nucleus where ribosomal RNA (rRNA) is produced and ribosomal subunits are assembled. Mitochondria are essential for cellular respiration and ATP synthesis. Lysosomes are responsible for digesting wastes and defective proteins.

Example Question #13 : Understand Steps Of Transcription

Which of the following is directly produced during gene transcription by RNA polymerase?

Possible Answers:

miRNA

rRNA

htRNA

mRNA

Correct answer:

htRNA

Explanation:

When RNA polymerase binds to a template strand of DNA, it recruits complementary ribonucleotides to form a strand of RNA. This strand of RNA, however, is incomplete and must undergo post-transcriptional modification to become a mature mRNA product. The initial RNA transcript is known as heteronuclear RNA, or htRNA.

Introns are removed for the htRNA and a 5'cap and poly-A tail are added to convert it to mRNA.

Example Question #14 : Understand Steps Of Transcription

Which of the following is not an example of post-transcriptional modification of a primary strand of mRNA?

Possible Answers:

All of these are post-transcriptional modifications of mRNA

Removal of introns

Addition of the 5' cap

Addition of the poly-A tail

Insertion of exons

Correct answer:

Insertion of exons

Explanation:

Following DNA transcription, the resulting RNA molecule must be modified before leaving the nucleus. Proteins in the nucleus add a 5' cap to the 5' end of the RNA strand, and a poly-A tail to the 3' end. These additions help prevent degradation of the transcript by any hydrolytic enzymes in the cytosol. Protein complexes called spliceosomes interact with the transcript to remove segments of non-coding RNA called introns. The remaining transcript following the excision of introns is composed only of coding segments of RNA, known as exons.

Though introns are removed during post-transcriptional modification, exons are not inserted. Rather, they are simply the remaining RNA sequences after the introns have been spliced out.

Example Question #14 : Understand Steps Of Transcription

Which of the following is the term that best describes a DNA strand that is used as a template for transcription? 

Possible Answers:

Non-coding strand

Sense strand

Non-template strand

Coding strand

Correct answer:

Non-coding strand

Explanation:

RNA polymerase binds and transcribes the “non-coding strand." It acts as a template that is used to generate a RNA transcript that is complementary to the DNA strand that was transcribed. The term “coding strand” refers to the DNA strand with the identical sequence to the newly synthesized RNA. The coding strand can also be called the “non-template strand” or the “sense strand.” 

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