Vaccinations have become a controversial topic in the United States. Currently the US Food and Drug Administration (FDA) regulates all vaccines. The federal government does not mandate vaccinations for any individual; however, all states require vaccinations for children entering public school. There are several types of vaccines—live attenuated vaccines, inactivated vaccines, subunit vaccines, toxoid vaccines, and conjugate vaccines, just to name a few. All of these vaccines have the shared purpose of exposing the host body to antigens of a specific disease. When the body receives the antigens, the immune system is activated, remembering the antigens. The next time the individual is exposed to the disease, the body will remember the antigen and have a better probability of not getting infected. Two scientists below discuss their belief on vaccines.

Scientist 1

Vaccines have saved many lives. The risks of not being vaccinated far outweigh the risks of adverse vaccine reactions. Reports linking autism to vaccines have been evaluated by the CDC, which states there is no scientific link between autism and vaccines. The second leading cancer killer in women is cervical cancer. The HPV vaccine protects against the two most common strains causing cancer. This is an example of a vaccine that does much more good than bad. Vaccines also reduce the amount of money spent on healthcare, because the preventative cost of a vaccine is much cheaper than the cost of treating an infected person. The only time a vaccine should not be administered is if the chance of the individual coming into contact with the disease is so rare it is not worth the potential of adverse reactions.

 Scientist 2

Many vaccines nowadays are extraneous. Vaccines for diseases like whooping cough and scarlet fever were once necessary but now outdated. Modern updates on hygiene, waste management, and water filtration have resulted in significantly decreased chances of infection. In addition, diseases like rotavirus have an infection period of a few days, and the main symptom is dehydration. Modern medicine can easily treat severe dehydration, and the risk of rotavirus infection is very slim; therefore, the results of infection are far milder than the results of an adverse reaction. Vaccines for children can cause extremely dangerous adverse reactions. This includes anaphylactic shock, paralysis, and death. While scientists have not been able to conclusively prove this, many believe that these reactions are related to the age of the host and the lack of a developed immune systemor neural network. Vaccines suppress the immune system, which can lead to autoimmune disorders. In addition, vaccines can congest the lymphatic system with proteins molecules from the vaccines; therefore, I would recommend requirements for vaccination to take place at a later stage in a child’s development.

What statement would Scientist 2, but not Scientist 1, agree with? 

In the 1980’s, an epidemic of bovine spongiform encephalopathy, or mad cow disease, swept through cattle herds in the United Kingdom. Scientists and veterinarians were troubled and had a difficult time managing the disease because it spread from one animal to another, and behaved differently than other diseases in the past. 

When infectious material from affected animals was treated with high levels of radiation, for example, the material remained infectious. All known bacteria or viruses that carry disease would have been killed by such a treatment. Additionally, some animals developed the disease without first being exposed to sick animals. Perhaps most frustratingly, among those animals that are exposed before becoming sick, it can take many years after exposure for illness to appear.

There quickly emerged two distinct explanations for the disease. 

Scientist 1:

Mad cow disease is unlike any disease we have handled before. It is increasingly clear that the best explanation for the disease’s dynamics involve proteins, called the protein-only hypothesis. These protein molecules are likely causative of the disease, and they lack any DNA or RNA. It is damage to these DNA or RNA molecules that kills bacteria or viruses when exposed to high levels of radiation. The most important observations that made scientists consider a unique, protein-only model for this disease involved its resistance to radiation. Remarkably, this would be the first example of an infectious agent copying itself without DNA or RNA to mediate the process.

Moreover, some animals develop the disease spontaneously, without physically being infected by another animal. This suggests that internal disorder among protein molecules is a potential route to developing disease, and may be accelerated by exposure to other sick animals.

In fact, this is consistent with the proposed mechanism. It is likely that proteins fold incorrectly, and then influence proteins around them to take on this errant conformation. Some proteins may fold incorrectly by chance, which explains spontaneous disease development. It also explains the long course of disease, as it takes many years for enough proteins to fold incorrectly and result in observable disease.

Scientist 2:

The suggestion that mad cow disease is caused exclusively by protein, in the absence of DNA or RNA, is such a dramatic departure from accepted biological processes that it warrants careful scrutiny. Additionally, other more conventional explanations should be thoroughly investigated before coming to such a conclusion.

Some scientists have shown that very small particles resembling viruses are visible in infectious material under powerful microscopes. Additionally, these viruses are consistent in size and shape with known, highly radiation-resistant viruses called polyomaviruses. It takes much higher-than-typical doses of radiation to cause enough DNA damage to inactivate these viruses.

The observation that mad cow disease occurs spontaneously in some animals is also explained by the viral explanation. Many viruses exist in animals and humans for years, undetected and not causing any observable disease. Sickness or stress can make these viruses reactivate, offering the illusion of spontaneous illness. All of these observations are consistent with the viral hypothesis.

Assume that Scientist 2's hypothesis is correct. Which of the following would be most likely?

In the 1980’s, an epidemic of bovine spongiform encephalopathy, or mad cow disease, swept through cattle herds in the United Kingdom. Scientists and veterinarians were troubled and had a difficult time managing the disease because it spread from one animal to another, and behaved differently than other diseases in the past. 

When infectious material from affected animals was treated with high levels of radiation, for example, the material remained infectious. All known bacteria or viruses that carry disease would have been killed by such a treatment. Additionally, some animals developed the disease without first being exposed to sick animals. Perhaps most frustratingly, among those animals that are exposed before becoming sick, it can take many years after exposure for illness to appear.

There quickly emerged two distinct explanations for the disease. 

Scientist 1:

Mad cow disease is unlike any disease we have handled before. It is increasingly clear that the best explanation for the disease’s dynamics involve proteins, called the protein-only hypothesis. These protein molecules are likely causative of the disease, and they lack any DNA or RNA. It is damage to these DNA or RNA molecules that kills bacteria or viruses when exposed to high levels of radiation. The most important observations that made scientists consider a unique, protein-only model for this disease involved its resistance to radiation. Remarkably, this would be the first example of an infectious agent copying itself without DNA or RNA to mediate the process.

Moreover, some animals develop the disease spontaneously, without physically being infected by another animal. This suggests that internal disorder among protein molecules is a potential route to developing disease, and may be accelerated by exposure to other sick animals.

In fact, this is consistent with the proposed mechanism. It is likely that proteins fold incorrectly, and then influence proteins around them to take on this errant conformation. Some proteins may fold incorrectly by chance, which explains spontaneous disease development. It also explains the long course of disease, as it takes many years for enough proteins to fold incorrectly and result in observable disease.

Scientist 2:

The suggestion that mad cow disease is caused exclusively by protein, in the absence of DNA or RNA, is such a dramatic departure from accepted biological processes that it warrants careful scrutiny. Additionally, other more conventional explanations should be thoroughly investigated before coming to such a conclusion.

Some scientists have shown that very small particles resembling viruses are visible in infectious material under powerful microscopes. Additionally, these viruses are consistent in size and shape with known, highly radiation-resistant viruses called polyomaviruses. It takes much higher-than-typical doses of radiation to cause enough DNA damage to inactivate these viruses.

The observation that mad cow disease occurs spontaneously in some animals is also explained by the viral explanation. Many viruses exist in animals and humans for years, undetected and not causing any observable disease. Sickness or stress can make these viruses reactivate, offering the illusion of spontaneous illness. All of these observations are consistent with the viral hypothesis.

With which of the following statements would both Scientist 1 and Scientist 2 most likley agree?

The significant increase in atmospheric carbon dioxide since pre-industrial levels can be seen in the world’s oceans which absorb the CO₂ and in turn undergo changes in chemistry. The consequences of increased CO₂ include acidification of seawater and a decrease in carbonate ion (CO₃^2-) concentration.

Changes in seawater chemistry affect marine organisms. The early life stages of invertebrates, such as squid, may be particularly vulnerable to changes in carbon dioxide levels. Acting as both predator and prey, squid are a significant component of marine ecosystems.   For example, fish and sea birds, such as tuna and albatross, are dependent on squid as a source of prey. Furthermore, the fishing industry is impacted by the health of squid populations. California fisheries produce the majority of market squid.

In order to determine how increased levels of carbon dioxide affect the development of squid, eggs were hatched in two different conditions: normal (380 µatm) and elevated (2100 µatm) levels of CO₂. The time to hatch and the size of the larval mantle (the anatomical feature that includes the body wall and fins) were measured and recorded. Two trials were conducted for each carbon dioxide concentration.

Which of the following can be concluded from the passage?

A student is choosing an enzyme to use in order to complete a chemical reaction. Based on the diagram of enzyme kinetics, which enzyme should the student use?

A student is choosing an enzyme to use in order to complete a chemical reaction. Based on the diagram of enzyme kinetics, which enzyme is more efficient?

The rate of a reversible chemical reaction depends on many factors, including concentrations of the reactants and products, temperature, and presence of enzymes called catalysts. In the forward reaction, two reactants combine to form one product. However, in a reverse reaction, the product is broken down into the two reactants.

In order for a forward reaction to occur, the reactants moving around in the test tube must physically interact with each other. The more often reactants interact with each other, the more produce is formed in the same amount of time. The speed at which reactants combine into products (the rate of the reaction) can be calculated by dividing the amount of a chemical produced in a reaction (often measured in moles) by the time it takes to produce that amount.

In order to determine the effects of reactant and product concentration, temperature, and presence of catalysts on the rate of a reaction, a scientist studied the following reaction:

The scientist varied the conditions of the experiment and measured the rate of the reaction. The results are outlined in Table 1. The units of concentration are moles per liter.

If the moles of acid convertase were doubled, how would the rate of reaction change?

The rate of a reversible chemical reaction depends on many factors, including concentrations of the reactants and products, temperature, and presence of enzymes called catalysts. In the forward reaction, two reactants combine to form one product. However, in a reverse reaction, the product is broken down into the two reactants.

In order for a forward reaction to occur, the reactants moving around in the test tube must physically interact with each other. The more often reactants interact with each other, the more product is formed in the same amount of time. The speed at which reactants combine into products (the rate of the reaction) can be calculated by dividing the amount of a chemical produced in a reaction (often measured in moles) by the time it takes to produce that amount.

In order to determine the effects of reactant and product concentration, temperature, and presence of catalysts on the rate of a reaction, a scientist studied the following reaction:

The scientist varied the conditions of the experiment and measured the rate of the reaction. The results are outlined in Table 1. The units of concentration are moles per liter.

What is a possible unit of a rate of reaction?

Both gases and liquids are considered to be fluids that have individual molecules that move around with kinetic and potential energy. Kinetic energy, defined as the energy related to motion, takes three forms: translational energy that occurs as a molecule moves from position A to position B, rotational energy that occurs as a molecule spins around an imaginary axis at its center of mass, and vibrational energy that occurs as individual atoms in a molecular bond move towards and away from each other. Usually, molecules possess varying combinations of kinetic energy forms. In contrast, potential energy is defined as stored energy that could be released to become kinetic energy. The total energy of a molecule is fixed, meaning that a molecule has some combination of kinetic and potential energies.

Varying amount of kinetic and potential energies define how molecules in a fluid interact with each other. For example, when the kinetic energy of a molecule is high (greater than 1000J), it can no longer interact with neighboring molecules strongly enough to remain a liquid. However, if the potential energies are too high (greater than 1000 J), molecules cannot escape a liquid to become a gas. If the kinetic energy is high and the potential energy is low, molecules tend to become a gas and can be modeled by an equation known as the Ideal Gas Law:

Where P is the pressure of a gas, V is the volume, n is the number of moles of a gas, R is a constant, and T is temperature in degrees Kelvin.

The Ideal Gas Law perfectly applies to particles with no mass, no intermolecular interactions, and no true volume. However, real molecules do not adhere perfectly to the Ideal Gas Law.

At a constant pressure and volume, the relationship between the number of moles and temperature may best be modeled as: 

Both gases and liquids are considered to be fluids that have individual molecules that move around with kinetic and potential energy. Kinetic energy, defined as the energy related to motion, takes three forms: translational energy that occurs as a molecule moves from position A to position B, rotational energy that occurs as a molecule spins around an imaginary axis at its center of mass, and vibrational energy that occurs as individual atoms in a molecular bond move towards and away from each other. Usually, molecules possess varying combinations of kinetic energy forms. In contrast, potential energy is defined as stored energy that could be released to become kinetic energy. The total energy of a molecule is fixed, meaning that a molecule has some combination of kinetic and potential energies.

Varying amount of kinetic and potential energies define how molecules in a fluid interact with each other. For example, when the kinetic energy of a molecule is high (greater than 1000J), it can no longer interact with neighboring molecules strongly enough to remain a liquid. However, if the potential energies are too high (greater than 1000 J), molecules cannot escape a liquid to become a gas. If the kinetic energy is high and the potential energy is low, molecules tend to become a gas and can be modeled by an equation known as the Ideal Gas Law:

Where P is the pressure of a gas, V is the volume, n is the number of moles of a gas, R is a constant, and T is temperature in degrees Kelvin.

The Ideal Gas Law perfectly applies to particles with no mass, no intermolecular interactions, and no true volume. However, real molecules do not adhere perfectly to the Ideal Gas Law.

At a constant pressure, the relationship between volume and temperature may best be shown as: