GRE Subject Test: Biochemistry, Cell, and Molecular Biology : Differentiation

Study concepts, example questions & explanations for GRE Subject Test: Biochemistry, Cell, and Molecular Biology

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All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

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Example Questions

Example Question #141 : Cell Biology

During asymmetric cell division, cells divide such that the mitotic spindles are oriented perpendicular to the plane on which the cells reside. What types of cells are the original parent cells and how does this affect cell lineage?

Possible Answers:

Asymmetric cell division begins the differentiation process from "parent" stem cells

Asymmetric cell division is proliferation process "parent" stem cells

Asymmetric cell division begins the de-differentiation process from "parent" differentiated cells

Asymmetric cell division is proliferation from "parent" differentiated cells

Asymmetric cell division begins the differentiation process from "parent" immune cells

Correct answer:

Asymmetric cell division begins the differentiation process from "parent" stem cells

Explanation:

The correct answer is that asymmetric cell division begins the differentiation process from "parent" stem cells. Symmetric cell division generates two identical daughter cells that have mitotic spindles oriented parallel to the plane on which the cells reside. However, cells that begin a differentiation lineage undergo asymmetric cell division. The perpendicularity of the mitotic spindle in cells that eventually differentiate contributes to this process. 

Example Question #1 : Help With Stem Cells

Which is the following answers best describes the key difference between totipotent and pluripotent stem cells?

Possible Answers:

Pluripotent stem cells can only form a very limited subset of tissue types depending on their origin; totipotent stem cells have the potential to become any of the three embryonic germ layers. 

Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism. 

Totipotent and pluripotent stem cells are different names for the same cell type, and thus there is no difference in their potential. 

Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells only have the potential to become blood and bone cell types. 

Pluripotent stem cells have the potential to become an entire organism; totipotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism. 

Correct answer:

Totipotent stem cells have the potential to become an entire organism; pluripotent stem cells can form any of the three embryonic germ layers, but cannot give rise to an entire organism. 

Explanation:

Totipotent stem cells are the stem cells with the greatest potential of all stem cell types. A totipotent stem cell can give rise to any embryonic cell type, and can ultimately form an entire organism. Pluripotent stem cells can become any of the three embryonic germ layers, however, they do not have the capacity to form an entire organism because they cannot give rise to the extraembryonic tissue required for development, such as the placenta. 

Example Question #1 : Help With Growth Factors

Which of the following mutations in the EGF pathway could lead to increased proliferation of cells?

Possible Answers:

Mutation abolishing kinase activity of RAF

Mutation causing constitutively phosphorylated Erk

Mutation blocking EGF binding to EGFR

Mutation introducing hydrophillic domains in the hydrophobic region of EGFR 

Correct answer:

Mutation causing constitutively phosphorylated Erk

Explanation:

The phosphorylation of Erk is the final step of the protein cascade of EGF pathway, and phosphorylated Erk enters the nucleus to increase transcription of genes that induce proliferation. If Erk is constitutively active, it will likely lead to higher proliferation rate.

Preventing EGF from binding to EGFR or disrupting EGFR's ability to enter the membrane would abolish EGF pathway activity and reduce proliferation. Likewise, abolishing kinase activity of RAF would terminate the signal transduction and lead to reduced proliferation.

Example Question #2 : Help With Growth Factors

Production of which of the following cytokines is stimulated when the  concentration decreases in the circulatory system?

Possible Answers:

Interleukin 2

Tumor necrosis factor

Interferon type II

Erythropoietin

Transforming growth factor beta 1

Correct answer:

Erythropoietin

Explanation:

Erythropoietin, released by the kidney, stimulates the production of red blood cells, which becomes necessary if circulating  has decreased. Tumor necrosis factor stimulates systemic inflammation and regulates the immune system. Transforming growth factor beta 1 controls cell growth, proliferation, differentiation and other processes. Interferon type II modulates immune functions. Interleukin 2 also modulates the immune cells.

Example Question #144 : Cell Biology

Which of the following growth factors is primarily produced by the kidney and is essential for the production of red blood cells?

Possible Answers:

Insulin-like growth factor

Angiopoietin

Erythropoietin

Fibroblast growth factor

Myostatin

Correct answer:

Erythropoietin

Explanation:

Erythropoietin is a glycoprotein that is crucial for the production of red blood cells, a process also called "erythropoiesis." Each of the other answers contains a growth factor, but none of these have a primary function in red blood cell production. 

Example Question #141 : Cell Biology

Which of the following cells is not correctly matched to the proper classification of cell (permanent, stable, labile)?

Possible Answers:

Bone marrow - labile

Cardiac muscle - permanent

Neuron - labile

Hepatocytes - stable

Correct answer:

Neuron - labile

Explanation:

Permanent cells remain in G0 and do not divide. They regenerate from stem cells, but cannot undergo division themselves. Neurons, skeletal muscle, cardiac muscle, and red blood cells are permanent cells.

Stable (quiescent) cells enter G1 from G0 when stimulated by introduction of the protein cyclin. Hepatocytes and lymphocytes are examples of stable cells.

Labile cells never go into G0 and divide rapidly with a short G1. Bone marrow, gut epithelium, skin, and hair follicles are examples of labile cells.

Example Question #142 : Cell Biology

Activation of the notch receptor by its ligand, delta, in neighboring cell populations defines patterns of distinct differentiated cell types among cells that have the potential to adopt the same fate. What is this process known as? 

Possible Answers:

Morphallaxis

Invagination 

None of these

Lateral inhibition 

Delamination 

Correct answer:

Lateral inhibition 

Explanation:

The correct answer is lateral inhibition. Also known as lateral specification, is a process that defines distinct cell types. This process is particularly important in vertebrate embryo nervous system development. Notch signaling is important for many developmental processes. Typically, lateral inhibition involves signaling through the notch receptor, which is stimulated by neighbor cells expressing the notch ligand, delta. Given that only neighboring cells can activate this signaling pathway, defined neighborhoods of undergo differentiation to become a distinct cell type. Morphollaxis is a term that describes regeneration of limbs without cell proliferation. Delamination describes mitotically dividing cells that split one cellular layer into two parallel layers. Furthermore, in terms of development, invagination is the initial step of gastrulation that reorganizes the embryo into a multi-layered organism with a body cavity. 

Example Question #143 : Cell Biology

Which term best describes when a cell has two stable equilibrium states, but can only exist in one state at a time? States are often phenotypes. 

Possible Answers:

Lateral inhibition

Bistability

Hysteresis

Differentiation

Quiescence

Correct answer:

Bistability

Explanation:

The correct answer is bistability. Bistability is a phenomenon that is observed in signaling pathways and cell commitment to one cell fate over another cell fate by differentiation, however, either cell fate is equally likely. Lateral inhibition occurs when cells signal to neighboring cells to effect a physiological change. Quiescence is a resting, non-dividing cell state. Hysteresis is the retention of a cell-state due to feedback signaling even in the absence of the initial stimulus. 

All GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

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