As one of the most revered figures in Christianity, the Virgin Mary was a constant theme of medieval religious art. In order to function as a highly visible symbol in a mostly illiterate culture, many different symbols were used to distinguish Mary. Most important was her robe, which was typically a bright blue color, which denoted expensive silks.

Saint Stephen is traditionally considered the first martyr of Christianity, and his death by stoning is told in the biblical book of Acts. Due to the circumstances of his death, he was usually depicted with a stone implanted in his skull. Thanks to his position as "the first martyr," Saint Stephen very commonly depicted in Medieval art.

While Christian iconography and themes feature as the most common subjects in Western art, certain periods were more heavily indebted to Christian tradition. During the period of Gothic art, which was roughly synonymous with the twelfth, thirteenth, and fourteenth centuries in Europe, Christian themes were almost the only ones used. Even in royal portraits, such as the Wilton Diptych from England, featured saints and angels next to the King.

Sandro Botticelli's The Birth of Venus is one of the signature paintings of Early Renaissance art from Italy, and as such deals with many themes prominent in the era. Botticelli chose the ancient goddess of love, appearing nude and fully formed from the ocean on a clam shell, instead of a Christian theme. Additionally, the picture's symbolism and color focus more on Platonic ideals than the Christian symbolism that was so prevalent in late Medieval art.

The Quran, the Islamic holy book, made Muslim artists pursue calligraphy for two different but related reasons. Calligraphy was the easiest mode of transmission for the written Quran in the early years of the religion. Additionally, Quranic prohibitions on creating figurative art made many Muslim artists pursue the geometrical and literary art of calligraphy.

During the Renaissance, with a renewed focus on classical art and scientific understanding, visual arts underwent a significant change. In particular, religious themes dwindled in importance in comparison to classical subjects, and deeper, more varied colors were used to represent more realistic depictions of the human form.

A triptych, from Greek words meaning "three fold," was often placed at the altar of medieval and early modern European churches. The paintings allowed for three separate scenes to be shown in one place, which allowed both the artist to depict three related topics and show the viewer a larger scene than one panel would allow. Triptychs were largely used for religious topics in the middle ages, but have remained a popular style up to the present day.

Among the most important and widely painted images in Medieval art were depictions of the Madonna and Child. These paintings, depicting the Virgin Mary and the infant Jesus, were meant to show the two holiest figures in Medieval Catholicism to the illiterate believers of the church in the middle ages.

Using newly found mathematical principles in their artwork, Renaissance artists were able to create perspective in Western painting for the first time. Vanishing-point perspective, which creates lines of sight that reflect the depth seen in reality, allowed for portraiture, religious subjects, and other works to be seen in new ways. Coupled with new scientific knowledge and an increasing use of dark colors and shadows, Renaissance art created new perceptions of artwork.

Perspective, the ability to place objects and figures in a 2D image that reflects 3D realities, was one of the driving forces of the artistic era known as the Renaissance. The era included painters like Leonardo da Vinci, Botticelli, and Michelangelo, who developed modes of portraiture that revolutionized artistic depictions. With this innovation, most Western art can be divided between pre- and post-Renaissance styles.