Glucokinase specifically phosphorylates the six-carbon sugar glucose. It is involved in glycolysis, but only in hepatocytes; hexokinase is the main enzyme that phosphorylates glucose during the first reaction of glycolysis. Rather, glucokinase's main role is to phosphorylate glucose to glucose-1-phosphate during the process of glycogen synthesis. The other kinases involved in glycolysis is phosphofructokinase. Fructose and mannose are not phosphorylated by glucokinase. Also, note that hexokinase has a higher affinity for glucose than does glucokinase.

Phosphofructokinase catalyzes the third step in glycolysis transforming fructose 6-phosphate to fructose-1,6-bisphosphate.  It is an irreversible step, and it is one of the major regulatory points of glycolysis.  One way in which it controls the flow of glycolysis is that when there is a high level of ATP, PFK is inhibited.  This is because the ultimate goal of glycolysis is to make ATP.  Thus, if there is already a high level of ATP, glycolysis should slow down.

Hexokinase catalyzes the first step of glycolysis, and this step requires the input of an ATP molecule. This step is unfavorable, but the steps catalyzed by the rest of the enzymes listed as answer choices are favorable.

In the fourth step of glycolysis, the six-carbon molecule fructose-1,6-bisphosphate is cleaved into two separate three-carbon molecules: dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. This is catalyzed by the enzyme, aldolase.

Triose phosphate isomerase is responsible for converting dihydroxyacetone phosphate into glyceraldehyde-3-phosphate. It is glyceraldehyde-3-phosphate that continues on through glycolysis to ultimately form a pyruvate molecule. Therefore, if there is no triose phosphate isomerase, the dihydroxyacetone will be unable to continue through glycolysis. The normal net yield of 2 ATP will be halved, the production of NADH will be halved, and only 1 pyruvate molecule will be created. Glycolysis will still be able to function, and the energy investment phase will be unaffected.

Phosphofructokinase-2 converts fructose-6-phosphate to fructose-2,6-bisphosphate. The product, fructose-2,6-bisphosphate activates phosphofructokinase-1, the rate limiting step in glycolysis. Phosphofructokinase-2 is regulated by insulin (activated) and glucagon (inhibited).

Glucose-6-phosphate (G6P) is the first molecule of the pentose phosphate pathway where it is acted upon by glucose-6-phosphate dehydrogenase. G6P is the result of the hexokinase (first) reaction in glycolysis. What is key here is that the tissue in question is muscle. Because muscle cells lack the glucose-6-phosphatase necessary to produce free glucose from G6P, they cannot be said to perform gluconeogenesis. They do, however, perform glycogenesis through conversion of G6P to glucose-1-phosphate followed by conversion to uridine diphosphateglucose for addition to a growing molecule of glycogen. 

Sucrose is the linking of glucose and fructose. Recall from the glycolytic pathway that fructose is further downstream than glucose, and therefore would allow for faster production of ATP.  

First, we must realize that the first committed step is the first irreversible reaction of glycolysis that is unique to glycolysis (cannot lead to another process, such as the pentose phosphate pathway). This is the third step, in which fructose-6-phosphate is converted to fructose-1,6-bisphosphate (the correct answer).  

Glucose is the beginning reactant of glycolysis, and pyruvate is the final product. Glucose-6-phosphate is the product of the first step of glycolysis overall, but not of the committed step.

Conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate is mediated by phosphoglycerate kinase. Conversion of phosphoenolpyruvate to pyruvate is mediated by pyruvate. In both these reactions adenosine diphosphate (ADP) is converted to ATP via substrate level phosphorylation. Conversion of 2-phosphoglycerate to phosphoenolpyruvate, mediated by enolase, does not produce ATP.