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Example Questions
Example Question #911 : Biochemistry
Which of the following metabolic processes directly requires oxygen?
Pyruvate dehydrogenase complex
Glycolysis
Citric acid cycle
Tricarboxylic acid cycle
Electron transport system
Electron transport system
The electron transport system is the only metabolic process listed that directly requires molecular oxygen. Oxygen is the final electron acceptor (it is one of the most electronegative atoms in our bodies) in the electron transport chain. This is the same as saying that oxygen has the highest reduction potential, and is capable of receiving electons. If oxygen is not present to accept the electron from the final enzyme complex in the inner mitochondrial membrane, then electron transport will be inhibited and thus no ATP will be produced via chemiosmosis.
Note that the Krebs cycle, citric acid cycle, and tricarboxylic acid cycle (TCA cycle) all refer to the same process, and do not directly require oxygen (oxygen is neither a reactant nor a product in any of the steps). However, oxygen is indirectly required, as there is no point to this cycle without subsequent oxidative phosphorylation. Thus in the absence of oxygen, of the choices shown, only glycolysis will proceed uninhibited.
Example Question #45 : Electron Transport And Oxidative Phosphorylation
Which phase of cellular respiration accounts for the highest production of energy?
Krebs Cycle
Glycolysis
Electron transport chain
Fermentation
Electron transport chain
The electron transport chain generates the most ATP out of all three major phases of cellular respiration. Glycolysis produces a net of 2 ATP per molecule of glucose. In the Krebs cycle, there is one GTP (which is an ATP equivalent) generate in the conversion of succinyl-CoA to succinate. However, the majority of the ATP produced during cellular respiration occurs at the electron transport chain by the reduction of coenzymes NADH and . This subsequently results in the generation of the proton motive force which ATP synthase uses to generate ATP from one unit of ADP and one unit of inorganic phosphate.
Example Question #1 : Other Oxidatative Phosphorylation Concepts
Where does oxidative phosphorylation take place in a prokaryote?
Endoplasmic reticulum
Cell membrane
Nucleus
Mitochondria
Cell wall
Cell membrane
In a eukaryote, oxidative phosphorylation occurs in the mitochondria because this is where the cell is able to set up a proton gradient. However, prokaryotes do not have mitochondria - they have no membrane-bound organelles at all. Therefore, the proton gradient that drives ATP synthesis in oxidative phosphorylation is created across the cell membrane.
Example Question #45 : Electron Transport And Oxidative Phosphorylation
If an uncoupler allows for excess buildup of protons inside of the mitochondrial matrix, which of the following processes will be inhibited?
Electron transport chain
Glycolysis
Krebs cycle
None of these will be inhibited
Pyruvate dehydrogenase complex
None of these will be inhibited
With the excess buildup of protons in the matrix, the only thing that will be inhibited is the generation of ATP by ATP synthase. The other processes in cellular respiration focus more on creation of high energy electron carriers, and therefore will continue as normal.
Example Question #46 : Electron Transport And Oxidative Phosphorylation
In oxidative phosphorylation, electrons are transferred from NADH and FADH2 to electron acceptors. This is one example of an oxidative process. Which of the following processes within another biochemical process could be considered oxidation?
Removing hydrogens
Removing oxygen
Adding electrons
Adding hydrogens
None of these choices are oxidation
Removing hydrogens
Predictably, a gain in oxygen is known as oxidation, while a loss of oxygen is reduction. Hydrogen follows the opposite pattern as oxidation: removing hydrogen is oxidation, while gaining hydrogen is reduction. Therefore, the correct answer is that removing hydrogens is considered oxidation.
In order to differentiate between oxidation and reduction in terms of electron transfer, it is helpful to remember the phrase "LEO the tiger says GER". A loss of electrons is oxidation, while a gain of electrons is reduction.
Example Question #46 : Electron Transport And Oxidative Phosphorylation
What is the major role of oxidative phosphorylation in cellular respiration?
To oxidize glucose to pyruvate.
To produce from the high energy electron carriers and .
To produce the high energy electron carriers and .
None of these choices are correct.
To produce through substrate level phosphorylation.
To produce from the high energy electron carriers and .
During oxidative phosphorylation, is created from the previously created and . All of the other choices describe other parts of cellular respiration. In glycolysis, glucose is oxidized to pyruvate. In both glycolysis and the Krebs cycle, substrate level phosphorylation occurs. Likewise, and are produced during glycolysis and the Krebs cycle, but not during oxidative phosphorylation, where these high energy electrons are passed down a series of membrane-bound enzymes to oxygen meanwhile protons are pumped into the intermembrane space of the mitochondria.
Example Question #1 : Other Oxidatative Phosphorylation Concepts
Which of the following steps represents a correct source of carbon dioxide during aerobic respiration?
Glycolysis
Citric acid cycle
Oxidative phosphorylation
All of these steps are sources of carbon dioxide
Fermentation
Citric acid cycle
To answer this question, it's important to have familiarity with the process of aerobic respiration.
In the first major pathway, glycolysis is split into two molecules of pyruvate through a series of reactions. Along the way, high-energy electron carriers are produced, along with ATP.
In the next major step, pyruvate is transferred into mitochondria, where it is decarboxylated into acetyl-CoA, with a concomitant production of NADH and carbon dioxide. Hence, this is a step that produces carbon dioxide. However, it is not found in the answer choices.
The third major component of aerobic respiration is the citric acid cycle. Here, the acetyl-CoA from the previous step is completely ripped apart to provide a great deal of energy. This huge amount of energy that is liberated is because the two carbon atoms that make up the acetyl group of acetyl-CoA become completely oxidized into two molecules of carbon dioxide. In terms of the energy liberated from the cycle, ATP along with a good deal of high-energy electron carriers are produced. This component of aerobic respiration is indeed a source of carbon dioxide.
Fermentation is an anaerobic pathway and is thus not the correct answer. Depending on the organism, carbon dioxide may or may not be produced.
Finally, aerobic respiration culminates in oxidative phosphorylation. Here, all of the high energy carriers from the previous steps are fed into the electron transport chain, resulting in the production of a great amount of ATP, the main energy currency of cells. In this final major step, it is oxygen gas that is produced, not carbon dioxide.
Example Question #51 : Electron Transport And Oxidative Phosphorylation
Which of the following correctly matches the phase of cellular respiration with its location in the cell?
Pyruvate dehydrogenase complex . . . mitochondrial matrix
Electron transport chain . . . cytoplasm
Electron transport chain . . . mitochondrial matrix
Krebs cycle . . . intermembrane space
Glycolysis . . . mitochondrial matrix
Pyruvate dehydrogenase complex . . . mitochondrial matrix
Glycolysis occurs in the cytoplasm. Pyruvate dehydrogenase complex occurs in the mitochondrial matrix. Krebs cycle occurs in the mitochondrial matrix. Electron transport chain protein complexes are embedded in the mitochondrial inner membrane.
Example Question #1 : Citric Acid Cycle
Which enzyme catalyzes the conversion of citrate to isocitrate?
Aldolase
Citrate isomerase
Aconitase
Citrate synthase
Phosphate
Aconitase
Aconitase is the enzyme that catalyzes the conversion of citrate to isocitrate. This essential enzyme is vital in energy production, as it acts like an iron regulatory protein. The conversion of citrate to isocitrate is important since it is needed to react with isocitrate dehydrogenase.
Example Question #2 : Citric Acid Cycle
What is the name of the enzyme that incorporate Acetyl-CoA into the citric acid cycle?
Pyruvate dehydrogenase
Pyruvate carboxylase
Citrate synthase
Acetyl-CoA carboxylase
Thiolase
Citrate synthase
Citrate synthase is the first enzyme of the citric acid cycle. Its role is to condense acetyl-CoA onto oxaloacetate in order to generate citrate.
Acetyl-CoA carboxylase is an enzyme that attaches a carboxyl group to acetyl-CoA in order to generate malonyl-CoA, which plays a role in fatty acid synthesis by contributing two carbons at a time to the growing hydrocarbon chain. Moreover, malonyl-CoA also serves a regulatory role in the breakdown and synthesis of fatty acids. Since it is a major precursor to the synthesis of fatty acids, high levels of it inhibit the breakdown of fatty acids by preventing fatty acids from entering the mitochondria, where they are broken down via beta-oxidation. Thus, malonyl-CoA allows fatty acids to be synethesized without simultaneously being degraded.
Thiolase is an enzyme that condenses two molecules of acetyl-CoA into acetoacetyl-CoA. This molecule is an important intermediate in two important pathways. One is the production of ketone bodies, while the other is the mevalonate pathway, which is an important series of reactions that synthesizes many compounds, such as cholesterol.
Pyruvate dehydrogenase is an enzyme complex that converts pyruvate into acetyl-CoA, thus linking glycolysis with the citric acid cycle.
Pyruvate carboxylase is an enzyme that adds a carboxyl group to pyruvate in order to generate oxaloacetate. This reaction can be used either to generate oxaloacetate for use in the kreb's cycle, or in the gluconeogenesis pathway to synthesize glucose from a variety of substrates.
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