Regulation of Gene Transcription - AP Biology
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What does the sigma factor do in bacterial transcription?
What does the sigma factor do in bacterial transcription?
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Guides RNA polymerase to the promoter. Helps RNA polymerase recognize specific promoters.
Guides RNA polymerase to the promoter. Helps RNA polymerase recognize specific promoters.
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What is the purpose of alternative splicing?
What is the purpose of alternative splicing?
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Generates different mRNA variants from the same gene. Increases protein diversity from a single gene.
Generates different mRNA variants from the same gene. Increases protein diversity from a single gene.
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What is the function of a silencer in gene regulation?
What is the function of a silencer in gene regulation?
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Decreases transcription by binding repressor proteins. Regulatory elements that reduce gene expression.
Decreases transcription by binding repressor proteins. Regulatory elements that reduce gene expression.
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Identify the effect of histone deacetylation on transcription.
Identify the effect of histone deacetylation on transcription.
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Decreases transcription by tightening DNA-histone interaction. Compacts chromatin, reducing gene accessibility.
Decreases transcription by tightening DNA-histone interaction. Compacts chromatin, reducing gene accessibility.
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Define an operon.
Define an operon.
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A cluster of genes regulated together. Found in prokaryotes for coordinated gene control.
A cluster of genes regulated together. Found in prokaryotes for coordinated gene control.
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What is the function of the 5' cap in mRNA?
What is the function of the 5' cap in mRNA?
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Protects mRNA from degradation and aids ribosome binding. Stabilizes mRNA and facilitates translation initiation.
Protects mRNA from degradation and aids ribosome binding. Stabilizes mRNA and facilitates translation initiation.
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Identify the role of histone acetylation in gene transcription.
Identify the role of histone acetylation in gene transcription.
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Increases transcription by loosening DNA-histone interaction. Opens chromatin structure for gene accessibility.
Increases transcription by loosening DNA-histone interaction. Opens chromatin structure for gene accessibility.
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Identify the protein that unwinds DNA for transcription initiation.
Identify the protein that unwinds DNA for transcription initiation.
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Helicase. Separates DNA strands for RNA polymerase access.
Helicase. Separates DNA strands for RNA polymerase access.
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Which chemical modification of DNA is associated with repression of transcription?
Which chemical modification of DNA is associated with repression of transcription?
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DNA methylation. Silences genes by adding methyl groups to cytosine.
DNA methylation. Silences genes by adding methyl groups to cytosine.
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What is the main purpose of transcriptional activators?
What is the main purpose of transcriptional activators?
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Increase transcription by binding to enhancers. Promote gene expression by recruiting RNA polymerase.
Increase transcription by binding to enhancers. Promote gene expression by recruiting RNA polymerase.
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What is the primary role of the mediator complex in transcription?
What is the primary role of the mediator complex in transcription?
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Facilitates interaction between transcription factors and RNA polymerase. Bridges regulatory proteins with transcription machinery.
Facilitates interaction between transcription factors and RNA polymerase. Bridges regulatory proteins with transcription machinery.
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Which type of RNA is synthesized from DNA during transcription?
Which type of RNA is synthesized from DNA during transcription?
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mRNA, tRNA, and rRNA. All RNA types produced during transcription.
mRNA, tRNA, and rRNA. All RNA types produced during transcription.
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What is the role of a promoter region in gene transcription?
What is the role of a promoter region in gene transcription?
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Initiates transcription by binding RNA polymerase. Acts as the binding site where transcription begins.
Initiates transcription by binding RNA polymerase. Acts as the binding site where transcription begins.
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What is the function of RNA polymerase III in eukaryotes?
What is the function of RNA polymerase III in eukaryotes?
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Transcribes tRNA and some small RNAs. Synthesizes transfer RNA and regulatory RNAs.
Transcribes tRNA and some small RNAs. Synthesizes transfer RNA and regulatory RNAs.
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Which process involves the removal of introns from pre-mRNA?
Which process involves the removal of introns from pre-mRNA?
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RNA splicing. Removes non-coding sequences from pre-mRNA.
RNA splicing. Removes non-coding sequences from pre-mRNA.
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Which process can silence genes via RNA-induced silencing complex (RISC)?
Which process can silence genes via RNA-induced silencing complex (RISC)?
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RNA interference. Post-transcriptional gene silencing mechanism.
RNA interference. Post-transcriptional gene silencing mechanism.
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Which molecule is a transcription factor that regulates gene expression?
Which molecule is a transcription factor that regulates gene expression?
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Proteins that bind to DNA sequences. Regulatory proteins that control gene expression levels.
Proteins that bind to DNA sequences. Regulatory proteins that control gene expression levels.
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What is the function of the TATA box in eukaryotic promoters?
What is the function of the TATA box in eukaryotic promoters?
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Indicates where transcription starts by binding transcription factors. Core promoter element crucial for transcription initiation.
Indicates where transcription starts by binding transcription factors. Core promoter element crucial for transcription initiation.
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What is the function of an enhancer in transcription regulation?
What is the function of an enhancer in transcription regulation?
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Increases transcription levels by interacting with activators. DNA sequences that boost gene expression remotely.
Increases transcription levels by interacting with activators. DNA sequences that boost gene expression remotely.
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Which RNA polymerase is responsible for rRNA synthesis in eukaryotes?
Which RNA polymerase is responsible for rRNA synthesis in eukaryotes?
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RNA polymerase I. Produces ribosomal RNA in the nucleolus.
RNA polymerase I. Produces ribosomal RNA in the nucleolus.
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What is the consequence of a mutation in the TATA box?
What is the consequence of a mutation in the TATA box?
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Altered gene expression due to faulty transcription initiation. Disrupts normal transcription factor binding.
Altered gene expression due to faulty transcription initiation. Disrupts normal transcription factor binding.
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What is the role of an operator in an operon system?
What is the role of an operator in an operon system?
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Regulates transcription by interacting with repressors. DNA sequence that controls repressor binding.
Regulates transcription by interacting with repressors. DNA sequence that controls repressor binding.
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Identify the process where small RNAs regulate gene expression post-transcriptionally.
Identify the process where small RNAs regulate gene expression post-transcriptionally.
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RNA interference. Uses small RNAs to silence target genes.
RNA interference. Uses small RNAs to silence target genes.
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Which molecule can prevent transcription in an operon by binding to the operator?
Which molecule can prevent transcription in an operon by binding to the operator?
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Repressor. Blocks transcription when bound to operator.
Repressor. Blocks transcription when bound to operator.
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Identify the role of general transcription factors in eukaryotes.
Identify the role of general transcription factors in eukaryotes.
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Assist in forming the transcription initiation complex. Required for RNA polymerase binding and function.
Assist in forming the transcription initiation complex. Required for RNA polymerase binding and function.
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Which RNA polymerase transcribes mRNA in eukaryotes?
Which RNA polymerase transcribes mRNA in eukaryotes?
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RNA polymerase II. Specifically synthesizes messenger RNA transcripts.
RNA polymerase II. Specifically synthesizes messenger RNA transcripts.
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What does the sigma factor do in bacterial transcription?
What does the sigma factor do in bacterial transcription?
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Guides RNA polymerase to the promoter. Helps RNA polymerase recognize specific promoters.
Guides RNA polymerase to the promoter. Helps RNA polymerase recognize specific promoters.
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Which molecule acts as an inducer in the lac operon?
Which molecule acts as an inducer in the lac operon?
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Allolactose. Derived from lactose, activates the lac operon.
Allolactose. Derived from lactose, activates the lac operon.
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Identify the region where transcription terminates in prokaryotes.
Identify the region where transcription terminates in prokaryotes.
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Terminator sequence. Signals the end of transcription in bacteria.
Terminator sequence. Signals the end of transcription in bacteria.
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Which element is essential for transcription initiation in eukaryotes?
Which element is essential for transcription initiation in eukaryotes?
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Promoter. Required regulatory sequence for gene expression.
Promoter. Required regulatory sequence for gene expression.
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