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Genetics of Prokaryotes (2B) Practice Test
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Q1
In a study of transformation, a naturally competent bacterial strain (Strain X) was incubated with purified DNA from Strain Y carrying a point mutation in gyrA that confers ciprofloxacin resistance (CipR). Cultures were treated with DNase I either during the incubation or after 30 minutes, then plated on ciprofloxacin. CipR colonies were recovered only when DNase I was added after 30 minutes. Which statement is most consistent with the bacterial behavior described?
In a study of transformation, a naturally competent bacterial strain (Strain X) was incubated with purified DNA from Strain Y carrying a point mutation in gyrA that confers ciprofloxacin resistance (CipR). Cultures were treated with DNase I either during the incubation or after 30 minutes, then plated on ciprofloxacin. CipR colonies were recovered only when DNase I was added after 30 minutes. Which statement is most consistent with the bacterial behavior described?